A5083553229- Biosensors and Analytical Detection
- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- RNA regulation and disease
- Nanoplatforms for cancer theranostics
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Adenosine and Purinergic Signaling
- Silk-based biomaterials and applications
- Diabetes and associated disorders
- Calcium signaling and nucleotide metabolism
- Lysosomal Storage Disorders Research
- RNA modifications and cancer
- Chemical Synthesis and Analysis
- HIV Research and Treatment
University of New Mexico
2024-2025
Center for Global Health
2024-2025
University of Ottawa
2021-2024
The University of Texas at El Paso
2017
The spread of COVID-19 has affected billions people across the globe, and diagnosis viral infection still needs improvement. Because high immunogenicity abundant expression during infection, SARS-CoV-2 nucleocapsid (N) protein could be an important diagnostic marker. This study aimed to develop a label-free optical aptasensor fabricated with novel single-stranded DNA aptamer detect N protein. N-binding aptamers selected using asymmetric-emulsion PCR-SELEX their binding affinity...
Abstract INTRODUCTION Pathological accumulation of tau (pTau) contributes to various tauopathies, including Alzheimer's disease (AD), and correlates with cognitive decline. A rapid surge in tau‐targeted approaches via anti‐sense oligonucleotides, active/passive immunotherapies suggests that targeting p‐Tau is a viable strategy against tauopathies. METHOD We describe multi‐species validation our previously described Qß virus‐like particle (VLP)–based vaccine technology phosphorylated on...
Abstract Lysosomal damage induces stress granule (SG) formation. However, the importance of SGs in determining cell fate and precise mechanisms that mediate SG formation response to lysosomal remain unclear. Here, we describe a novel calcium-dependent pathway controlling formation, which promotes survival during damage. Mechanistically, calcium-activated protein ALIX transduces signals by eIF2α phosphorylation after sensing calcium leakage. enhances promoting association between PKR its...
Small membrane-derived extracellular vesicles have been proposed as participating in several cancer diseases, including breast (BC). We performed a phosphoproteomic analysis of cancer-derived small (sEVs) to provide insight into the molecular and cellular regulatory mechanisms important for tumor progression metastasis. examined three cell line models cancer: MCF10A (non-malignant), MCF7 (estrogen progesterone receptor-positive, metastatic), MDA-MB-231 (triple-negative, highly metastatic)....
Breast cancer (BC) is the second most frequently diagnosed and accounts for approximately 25% of new cases in Canadian women. Using biomarkers as a less-invasive BC diagnostic method currently under investigation but not ready practical application clinical settings. During last decade, extracellular vesicles (EVs) have emerged promising source because they contain cancer-derived proteins, RNAs, metabolites. In this study, EV proteins from small EVs (sEVs) medium (mEVs) were isolated...
SUMMARY Lysosomal damage poses a significant threat to cell survival. Our previous work has reported that lysosomal induces stress granule (SG) formation. However, the importance of SG formation in determining fate and precise mechanisms through which triggers remains unclear. Here, we show is initiated via novel calcium-dependent pathway plays protective role promoting survival response damage. Mechanistically, demonstrate during damage, ALIX, calcium-activated protein, transduces signals...
Membrane-derived extracellular vesicles, referred to as microvesicles (MVs), have been proposed participate in several cancer diseases. In this study, MV fractions were isolated by differential ultracentrifugation from a metastatic breast (BC) cell line MDA-MB-231 and non-cancerous MCF10A, then analyzed nano-liquid chromatography coupled tandem mass spectrometry. A total of 1519 proteins identified both lines. The data obtained compared previously small vesicles (sEVs), revealing 1272...
The aim of the research. To examine proteomic profi le breast cancer exosomes. Material and methods. Cell lines used for this study were MDA-MB-231 female epithelial cells (ATCC HTB-26) MCF10A non-tumorigenic tissue cells. MVs isolated using diff erential ultracentrifugation. Samples lysed, reduced, alkylated, digested, analyzed by an Orbitrap Fusion mass spectrometer. MS raw fi les MaxQuant version 1.6.12.0. Peptides searched against human UniProt FASTA database Andromeda search engine,...