Plant extracts have recently received increased attention as alternative sources of antimicrobial agents in the fight against multidrug-resistant bacteria. Non-targeted metabolomics liquid chromatography-quadrupole time-of-flight tandem mass spectrometry, molecular networking, and chemometrics were used to evaluate metabolic profiles red green leaves two Brassica juncea (L.) varieties, var. integrifolia (IR IG) rugosa (RR RG), well establish a relationship between elucidated chemical...

Abstract Alzheimer’s disease (AD) poses a global health challenge, demanding innovative approaches for effective treatments. Clerodendrum infortunatum Linn. (Lamiaceae) is shrub traditionally used as medicinal plant to treat inflammation, skin diseases, and bronchitis. This study aims identify the main bioactive metabolites in C. using LC-QTOF-MS/MS investigate its potential protecting against cognitive decline rats with scopolamine-induced AD disease. Metabolite profiling was performed on...

This study comprehensively characterized the metabolite profiles of six lettuce varieties and established correlation between elucidated their antivirulence effects. A total 195 metabolites were annotated using LC-QTOF-MS/MS metabolomics assisted by molecular networking integrated with chemometrics. Red (red longifolia lolla rosa) demonstrated higher chlorogenic chicoric acids suggesting antioxidant properties. In parallel, amino disaccharides enriched in romaine rationalizing its palatable...

Abstract Introduction This research explores sustainable applications for waste generated from fenugreek ( Trigonella foenum‐graecum ), a plant with both nutritional and medicinal uses. The study specifically targets components as potential sources of nutrients bioactive compounds. Objectives focus is to conduct detailed metabolic profiling waste, assess its anti‐inflammatory properties by studying cyclooxygenase (COX) inhibitory effect, correlate this effect the metabolite fingerprint....

With 28 potential N-glycosylation sites, human carcinoembryonic antigen (CEA) bears an extreme amount of N-linked glycosylation, and approximately 60% its molecular mass can be attributed to carbohydrates. CEA is often overexpressed released by many solid tumors, including colorectal carcinomas. displays impressive heterogeneity variability in sugar content; however, site-specific distribution carbohydrate structures has not been reported so far. The present study investigated samples...

Abstract Background Infections caused by Acinetobacter baumannii are becoming a rising public health problem due to its high degree of acquired and intrinsic resistance mechanisms. Bacterial lipases penetrate damage host tissues, resulting in multiple infections. Because there very few effective inhibitors bacterial lipases, new alternatives for treating A. infections urgently needed. In recent years, Brassica vegetables have received lot attention since their phytochemical compounds been...

Protein profiling of major bovine milk proteins (i.e., whey and casein proteins) is great interest in food science technology. This complex set protein proteoforms may vary with breed, genetics, lactation stage, health, nutritional status the animal. Current routine methods for at intact level are typically based on capillary electrophoresis-ultraviolet, which does not allow confirming unequivocally identity separated proteins. As an alternative, this study, we describe first time a novel...

Quinoa is widely recognized for its exceptional nutritional properties, particularly complete protein content. This study, the first time, investigates effects of processing methods (boiling and extrusion) farming conditions (conventional organic) on proteomic profile. Following a label-free shotgun proteomics approach, total 1796 proteins were identified quantified across all quinoa samples. Regarding processing, both boiling extrusion produced extracts with lower content, number decreasing...

In this work, an untargeted metabolomic approach based on sensitive analysis by on-line solid-phase extraction capillary electrophoresis mass spectrometry (SPE-CE-MS) in combination with multivariate data is proposed as efficient method for the identification of biomarkers Huntington's disease (HD) progression plasma. For purpose, plasma samples from wild-type (wt) and HD (R6/1) mice different ages (8, 12, 30 weeks), were analyzed C18 -SPE-CE-MS order to obtain characteristic electrophoretic...

Quinoa seed proteins are of prime importance in human nutrition and plant breeding for cultivar identification improvement. In this study, from seeds black, red, white quinoa Peru Bolivia (also known as royal) were extracted, digested analyzed by nano-liquid chromatography coupled to Orbitrap tandem mass spectrometry (LC-MS/MS). The raw spectra data processed label-free quantification (LFQ) using MaxQuant/Andromeda against a specific database National Center Biotechnology Information (NCBI)....

Quinoa proteins are attracting global interest for their wide amino acid profile and as a promising source the development of biomedical treatments, including those against immune-mediated diseases. However, information about bioactivity quinoa is scarce. In this study, grain proteome map obtained by label-free mass spectrometry-based shotgun proteomics was investigated identification with potential immunonutritional bioactivities, related to cancer. After carefully examining sequence...

On-line aptamer affinity solid-phase extraction direct mass spectrometry (AA-SPE-MS) is presented for the rapid purification, preconcentration, and characterization of α-synuclein (α-syn), which a protein biomarker related to Parkinson's disease. Valve-free AA-SPE-MS easily implemented using typical SPE microcartridges instrumental set-up necessary on-line capillary electrophoresis-mass (AA-SPE-CE-MS). The essential requirement substituting application separation voltage by pressure 100 mbar...

Protein precipitation and centrifugal filtration are well-established methods for concentrating purifying peptides with a low relative molecular mass (Mr) from human blood plasma before proteomic peptidomic studies using high-performance separation techniques, but there is little information on peptide recoveries. Here, we evaluate acetonitrile followed by range of conditions the analysis Mr in CE–MS SPE coupled online to CE–MS. Three opioid were used as model compounds, that is, dynorphin A...

The analysis by capillary electrophoresis (CE) of low abundant proteins in complex samples, such as biological fluids and food, is especially challenging, due to the poor concentration sensitivity microscale separation techniques sample matrix complexity. In order overcome these major drawbacks, microextraction preparation based on on-line solid-phase extraction (SPE-CE) are regarded an excellent alternative for clean-up analyte preconcentration with minimum handling. this study, we present,...

Transthyretin (TTR) is known to misfold and aggregate, causing different types of amyloidosis. Familial amyloidotic polyneuropathy type I (FAP‐I), which the most common hereditary systemic amyloidosis, associated with a TTR variant that presents single amino acid substitution valine for methionine at position 30 (Met 30). To screen TTR‐related amyloidosis rapidly reliably, we have developed novel procedure based on analysis monomers from homotetrameric protein (∼56 kDa). First, established...