- Genomics, phytochemicals, and oxidative stress
- Phytochemicals and Antioxidant Activities
- Biofuel production and bioconversion
- Genetic Mapping and Diversity in Plants and Animals
- Bioenergy crop production and management
- Crop Yield and Soil Fertility
- Antioxidant Activity and Oxidative Stress
- Plant Gene Expression Analysis
- Insect-Plant Interactions and Control
- Genetics and Plant Breeding
- Insect Pest Control Strategies
- Food composition and properties
- Moringa oleifera research and applications
- Plant Disease Resistance and Genetics
- Free Radicals and Antioxidants
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Wheat and Barley Genetics and Pathology
- Porphyrin and Phthalocyanine Chemistry
- Sugarcane Cultivation and Processing
- Plant Stress Responses and Tolerance
- Plant pathogens and resistance mechanisms
- Listeria monocytogenes in Food Safety
- Viral gastroenteritis research and epidemiology
- Allelopathy and phytotoxic interactions
University of Illinois Urbana-Champaign
2015-2024
Goodwin College
2003-2020
Urbana University
1988-2014
Oregon State University
2014
Energy Biosciences Institute
2013
Illinois Department of Natural Resources
1997-2009
Washington State University
1994-2007
University of Nottingham
2006
Louisiana Department of Natural Resources
2005
University of Wisconsin–Madison
1993-2003
Glucosinolates were evaluated in 5 groups and 65 accessions of Brassica oleracea (50 broccoli, 4 Brussels sprouts, 6 cabbage, 3 cauliflower, 2 kale) grown under uniform cultural conditions. their concentrations varied among the different within each group. The predominant glucosinolates broccoli 4-methylsulfinylbutyl glucosinolate (glucoraphanin), 3-butenyl (gluconapin), 3-indolylmethyl glucosinoate (glucobrassicin). Glucoraphanin concentration ranged from 0.8 μmol g-1 DW EV6-1 to 21.7...
Recent investigations into carotenoid and tocopherol biological activity in mammalian systems indicate that these antioxidants are associated with the prevention of degenerative diseases. Both carotenoids tocopherols can be found corn kernel tissue. A replicated survey 44 sweet dent lines was conducted to determine qualitative quantitative variability lutein, zeaxanthin, β-cryptoxanthin, α-carotene, β-carotene, as well α-, δ-, γ- forms tocopherol. The primary fresh market were lutein γ form...
Cruciferous vegetables contain high levels of vitamins that can act as antioxidants, compounds may protect against several degenerative diseases. The edible portions 50 broccoli and 13 cabbage, kale, cauliflower, Brussels sprouts accessions were assayed to determine variation in α-carotene, β-carotene, α-tocopherol, γ-tocopherol, ascorbate contents within between subspecies Brassica oleracea. Ascorbate content was estimated fresh samples using HPLC. Tissues for carotene tocopherol analysis...
In some cruciferous plants, epithiospecifier protein (ESP) directs myrosinase (EC 3.2.3.1)-catalyzed hydrolysis of alkenyl glucosinolates toward epithionitrile formation. Here, for the first time, we show that ESP activity is negatively correlated with extent formation health-promoting phytochemical sulforaphane in broccoli (Brassica oleracea L. ssp. italica). A 43 kDa and sequence homology to Arabidopsis thaliana was cloned from cv. Packman expressed Escherichia coli. a model system,...
Abstract Miscanthus is a perennial wild grass that of global importance for paper production, roofing, horticultural plantings, and an emerging highly productive temperate biomass crop. We report chromosome-scale assembly the paleotetraploid M. sinensis genome, providing resource links its chromosomes to related diploid Sorghum complex polyploid sugarcanes. The asymmetric distribution transposons across two homoeologous subgenomes proves paleo-allotetraploidy identifies several balanced...
Ten broccoli [ Brassica oleracea L. (Botrytis Group)] accessions were grown in several environments to estimate glucosinolate (GS) variability associated with genotype, environment, and genotype × environment interaction identify differences the stability of GSs florets. Significant genetic identified for aliphatic but not indolyl GSs. The percentage GS attributable individual compounds ranged from 54.2% glucoraphanin 71.0% progoitrin. For total GSs, was only 12%. Both qualitative...
Abstract Background Miscanthus (subtribe Saccharinae, tribe Andropogoneae, family Poaceae) is a genus of temperate perennial C4 grasses whose high biomass production makes it, along with its close relatives sugarcane and sorghum, attractive as biofuel feedstock. The base chromosome number (x = 19) different from that other Saccharinae approximately twice the related Sorghum bicolor 10), suggesting large-scale duplications may have occurred in recent ancestors . Owing to complexity genome...
Increasing consumer demand for natural ingredients in foods and beverages justifies investigations into more economic sources of colorants. In this study, 398 genetically diverse pigmented accessions maize were analyzed using HPLC to characterize the diversity anthocyanin composition concentration germplasm. One hundred sixty-seven identified that could produce anthocyanins kernel pericarp or aleurone classified compositional categories. Anthocyanin content was highest pericarp-pigmented...
Abstract Miscanthus × giganteus (Mxg) is an important bioenergy feedstock crop, however, genetic diversity among legacy cultivars may be severely constrained. Only one introduction from Japan to Denmark of this sterile, triploid, vegetatively propagated crop was recorded in the 1930s. We sought determine if Mxg North America were all synonyms, and they derived European introduction. used 64 nuclear five chloroplast simple sequence repeat ( SSR ) markers estimate similarity for 27 accessions...
Antioxidant capacity of hydrophilic and lipophilic extracts from eight broccoli genotypes was compared using the oxygen radical absorbance (ORAC) assay. Each genotype analyzed for carotenoid, tocopherol, ascorbic acid, flavonoid content. Results indicate that antioxidant ranged 65.8 to 121.6 μmol trolox equivalents (TE)/g tissue, 3.9 17.5 TE/g. Ascorbic acid content did not explain total variation in those extracts, suggesting either presence other components have yet be identified or known...
Journal Article Acylglucoses from Wild Tomatoes Alter Behavior and Reduce Growth Survival of Helicoverpa zea Spodoptera exigua (Lepidoptera: Noctuidae) Get access John A. Juvik, Juvik Search for other works by this author on: Oxford Academic PubMed Google Scholar Joseph Shapiro, Shapiro Todd E. Young, Young Martha Mutschler Economic Entomology, Volume 87, Issue 2, 1 April 1994, Pages 482–492, https://doi.org/10.1093/jee/87.2.482 Published: 01 1994 history Received: 25 February 1993 Accepted:...
Chemical measures of antioxidant activity within the plant, such as oxygen radical absorbance capacity (ORAC) assay, have been reported for many plant-based foods. However, extent to which chemical relate cellular oxidative stress is unclear. The natural variation in phytochemical content 22 broccoli genotypes was used determine correlations among composition (carotenoids, tocopherols and polyphenolics), (ORAC), antioxidation [prevention DNA damage oxidation biomarker dichlorofluorescein...
Effect of pre-harvest methyl jasmonate (MeJA) and post-harvest 1-methylcyclopropene (1-MCP) treatments on broccoli floret glucosinolate (GS) concentrations quinone reductase (QR, an in vitro anti-cancer biomarker) inducing activity were evaluated two days prior to harvest, at harvest 10, 20, 30 storage 4 °C. MeJA four heads was observed significantly increase ethylene biosynthesis resulting chlorophyll catabolism during reduced product quality. Post-harvest treatment with (1-MCP), which...
Abstract Information on genome size, ploidy level, and genomic polymorphisms among accessions of the genus M iscanthus can assist in taxonomic studies, help understand evolution genus, provide valuable information to biomass crop improvement programs. Taxonomic investigation combining variation plant morphology, chromosome numbers, simple sequence repeat ( SSR ) marker were applied characterize 101 accessions. A total 258 amplicons generated from 17 informative primer pairs was subjected...
Glucosinolates, their hydrolysis products and primary metabolites were analyzed in five pak choi cultivars to determine the effect of methyl jasmonate (MeJA) on metabolite flux from glucosinolates products. Among detected (total 14 glucosinolates; 9 aliphatic, 4 indole 1 aromatic glucosinolates), glucosinolate concentrations (153–229%) increased with MeJA treatment. Changes total isothiocyanates by associated epithiospecifier protein activity estimated as nitrile formation. Goitrin, a...