A5000467262- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Metabolism and Genetic Disorders
- Integrated Circuits and Semiconductor Failure Analysis
- Cholesterol and Lipid Metabolism
- Analytical Methods in Pharmaceuticals
- Hemoglobin structure and function
- Microbial Metabolic Engineering and Bioproduction
- PARP inhibition in cancer therapy
- Ferroptosis and cancer prognosis
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Systemic Lupus Erythematosus Research
- Advanced Chemical Sensor Technologies
- Liver Disease Diagnosis and Treatment
- Cardiac electrophysiology and arrhythmias
- Peroxisome Proliferator-Activated Receptors
- Sphingolipid Metabolism and Signaling
- Mass Spectrometry Techniques and Applications
- Diet and metabolism studies
- Advanced Proteomics Techniques and Applications
- Pesticide Residue Analysis and Safety
Molecular Discovery (United Kingdom)
2024-2025
University of Perugia
2021-2024
Leydig cells rely on lipids and fatty acids (FA) for essential functions like maintaining structural integrity, energy metabolism, steroid hormone synthesis, including testosterone production.
Tracing lipid metabolism in mammalian cells presents a significant technological challenge due to the vast structural diversity of lipids involved multiple metabolic routes. Biorthogonal approaches based on click chemistry have revolutionized analytical performance tracing. When adapted for mass spectrometry (MS), it enables highly specific and sensitive analyses transformations at lipidome scale. Here, we advance this approach by integrating liquid chromatography (LC) prior MS detection...
Tracing lipid metabolism in mammalian cells presents a significant technological challenge due to the vast structural diversity of lipids involved multiple metabolic routes. Biorthogonal approaches based on click chemistry have revolutionized analytical performance tracing. When adapted for mass spectrometry (MS), it enables highly specific and sensitive analyses transformations at lipidome scale. Here, we advance this approach by integrating liquid chromatography (LC) prior MS detection...
Untargeted metabolomics is a growing field, in which recent advances high-resolution mass spectrometry coupled with liquid chromatography (LC-MS) have facilitated untargeted approaches as result of improvements sensitivity, accuracy, and resolving power. However, very large amount data are generated. Consequently, using computational tools now mandatory for the in-depth analysis data. This article describes MetAbolomics ReSearch (MARS), an all-in-one vendor-agnostic graphical user...
Drugs must satisfy several protocols and tests before being approved for the market. Among them, forced degradation studies aim to evaluate drug stability under stressful conditions in order predict formation of harmful products (DPs). Recent advances LC-MS instrumentation have facilitated structure elucidation degradants, although a comprehensive data analysis still represents bottle-neck due massive amount that can be easily generated. MassChemSite has been recently described as promising...
Autoimmune diseases (AID), such as systemic lupus erythematosus (SLE) and sclerosis (SS), are complex conditions involving immune system dysregulation. Diagnosis is challenging, requiring biomarkers for improved detection prediction of relapses. Lipids have emerged potential due to their role in inflammation response. This study uses an untargeted C18 RP-LC-MS lipidomics approach comprehensively assess changes lipid profiles patients with SLE SS. By analyzing whole blood plasma, the aims...
Stress testing is one of the most important parts drug development process, helping to foresee stability problems and identify degradation products. One processes involving stress represented by forced studies, which can predict impact certain conditions pH, moisture, heat, or other negative effects due transportation packaging issues on potency purity, ensuring patient safety. Regulatory agencies have been working a standardization laboratory procedures since past two decades. results those...
Phosphatidylinositols (PIs) are complex lipids that play a key role in cell signaling. Like other phospholipids, they esterified with unsaturated fatty acyl residues (FAs), making them susceptible to modification by reactive oxygen and nitrogen species (RNS). Recent studies using mass spectrometry (MS)-based lipidomics approaches have revealed lipid nitration results plethora of structurally chemically modified (epilipids), including nitrated nitroxidized derivatives phosphatidylcholines,...