A5049572937- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Molecular Biology Techniques and Applications
- Isotope Analysis in Ecology
Maastricht University
2023-2025
Recently, a novel technology was published, utilizing the strengths of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) and immunohistochemistry (IHC), achieving highly multiplexed, targeted biomolecules in tissue. This new technique, called MALDI-IHC, opened up workflows to target molecules interest using MALDI-MSI that are usually by standard IHC. In this paper, utility MALDI-IHC its complementarity with untargeted on-tissue bottom-up spatial proteomics...
We introduce a novel approach for comprehensive molecular profiling in biological samples. Our single-section methodology combines quantitative mass spectrometry imaging (Q-MSI) and single step extraction protocol enabling lipidomic proteomic liquid chromatography tandem (LC-MS/MS) analysis on the same tissue area. The integration of spatially correlated data section allows interpretation landscape. Comparing Q-MSI Q-LC-MS/MS quantification results sheds new light effect MSI related sample...
Abstract Glycans play an important role in biology with multiple cellular functions ranging from cell signaling, mobility and growth to protein folding localization. The N-glycosylation state within a tissue has been found vary greatly between healthy diseased patients proven have clinical diagnostic value. Matrix assisted laser-desorption ionization (MALDI) mass spectrometry imaging (MSI) allows for untargeted analysis of biomolecules, including N-glycans, on section provides spatial...
In recent years, mass spectrometry-based imaging techniques have improved at unprecedented speeds, particularly in spatial resolution, and matrix-assisted laser desorption/ionization (MALDI) spectrometry (MSI) experiments can now routinely image molecular profiles of single cells an untargeted fashion. With the introduction MALDI-immunohistochemistry (IHC), multiplexed visualization targeted proteins their native tissue location has become accessible joins suite multimodal that help unravel...