A5067170951- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Genomics and Phylogenetic Studies
- Diet and metabolism studies
- Cancer, Lipids, and Metabolism
- Metabolism and Genetic Disorders
- Antifungal resistance and susceptibility
- Cancer-related molecular mechanisms research
- Mass Spectrometry Techniques and Applications
- Bioactive Compounds and Antitumor Agents
- Medical Imaging Techniques and Applications
- Nanoparticles: synthesis and applications
- Metabolism, Diabetes, and Cancer
- Cancer Genomics and Diagnostics
- Growth Hormone and Insulin-like Growth Factors
- Radiomics and Machine Learning in Medical Imaging
Innsbruck Medical University
2015-2021
Universität Innsbruck
2015-2021
Oroboros Instruments (Austria)
2015
Next generation sequencing (NGS) allows investigating mitochondrial DNA (mtDNA) characteristics such as heteroplasmy (i.e. intra-individual sequence variation) to a higher level of detail. While several pipelines for analyzing heteroplasmies exist, issues in usability, accuracy results and interpreting final data limit their usage. Here we present mtDNA-Server, scalable web server the analysis mtDNA studies any size with special focus on usability well reliable identification quantification...
Abstract Rewiring of energy metabolism and adaptation mitochondria are considered to impact on prostate cancer development progression. Here, we report mitochondrial respiration, DNA mutations gene expression in paired benign/malignant human tissue samples. Results reveal reduced respiratory capacities with NADH-pathway substrates glutamate malate malignant a significant metabolic shift towards higher succinate oxidation, particularly high-grade tumors. The load potentially deleterious...
Alterations of mitochondrial DNA (mtDNA) copy number have been associated with a wide variety phenotypes and diseases. Unfortunately, the literature provides scarce methodical information about duplex targeting nuclear mtDNA that meets quality criteria for qPCR. Therefore, we established method quantification using quantitative PCR assay allows simultaneous single gene (beta-2-microglobulin) t-RNALeu on mtDNA. We include plasmid containing both targets in order to normalize against...
Altered mitochondrial metabolism plays a pivotal role in the development and progression of various diseases, including cancer. Cell lines are frequently used as models to study (dys)function, but little is known about their respiration metabolic properties comparison primary tissue origin. We have developed method for assessment oxidative phosphorylation prostate samples only 2 mg wet weight using high‐resolution respirometry. Reliable protocols were established investigate respiratory...
Invasive fungal infections have significantly increased over the past decades in immunocompromised individuals and high-risk patients. Amphotericin B (AmB) exerts a powerful broad activity against vast array of fungi has remarkably low rate microbial resistance. However, most isolates Aspergillus terreus developed an intrinsic resistance AmB, during this study, we characterized mode action polyene antifungal drug more detail resistant (ATR) rare susceptible (ATS) clinical A. terreus.We...
Tumor cells adapt via metabolic reprogramming to meet elevated energy demands due continuous proliferation, for example by switching alternative sources. Nutrients such as glucose, fatty acids, ketone bodies and amino acids may be utilized preferred substrates fulfill increased requirements. In this study we investigated the characteristics of benign cancer prostate with respect their utilization medium chain (MCTs) long triglycerides (LCTs) under standard glucose-starved culture conditions...
Massive parallel sequencing technologies are promising a highly sensitive detection of low-level mutations, especially in mitochondrial DNA (mtDNA) studies. However, processes from extraction and library construction to bioinformatic analysis include several varying tasks. Further, there is no validated recommendation for the comprehensive procedure. In this study, we examined potential pitfalls on results based two-person mtDNA mixtures. Therefore, compared three polymerases, six different...
The IGF network with its main receptors receptor 1 (IGF1R) and insulin (INSR) is of major importance for cancer initiation progression. To date, clinical studies targeting this were disappointing call thorough analysis the in models. We highlight oncogenic effects controlled by IGF1R INSR prostate cells show similarities as well differences after knockdown (KD). In PC3 stably transduced inducible short hairpin RNAs, or attenuated cell growth proliferation ultimately driving into apoptosis....
Under aerobic conditions, some cancers switch to glycolysis cover their energy requirements. Taking advantage of this process, functional imaging techniques such as PET-CT can be used detect and assess tumorous tissues. The aim study was investigate standardized uptake values mitochondrial DNA mutations in oral squamous cell carcinoma. A cohort 57 patients underwent 18[F]FDG-PET-CT were collected. In 15 patients, data on the tumor available. Kaplan-Meier curves calculated, correlation...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)