A grand challenge in synthetic biology is to use our current knowledge of RNA science perform the automatic engineering completely sequences encoding functional RNAs living cells. We report here a fully automated design methodology and experimental validation interaction circuits working cellular environment. The computational algorithm, based on physicochemical model, produces novel by exploring space possible compatible with predefined structures. tested Escherichia coli designing several...

Small RNAs (sRNAs) can operate as regulatory agents to control protein expression by interaction with the 5′ untranslated region of mRNA. We have developed a physicochemical framework, relying on base pair energies, design multi-state sRNA devices solving an optimization problem objective function accounting for stability transition and final intermolecular states. Contrary analysis reaction kinetics ensemble sRNAs, we solve inverse finding sequences satisfying targeted reactions. show here...

Organisms have different circuitries that allow converting signal molecule levels to changes in gene expression. An important challenge synthetic biology involves the de novo design of RNA modules enabling dynamic processing live cells. This requires a scalable methodology for sensing, transmission, and actuation, which could be assembled into larger signaling networks. Here, we present biochemical strategy RNA-mediated transduction cascades able sense small molecules RNAs. We switchable...

Resource allocation is essential to selection and implementation of innovative projects in science technology. Current "winner-take-all" models for grant applications require significant researcher time writing extensive project proposals, rely on the availability a few time-saturated volunteer experts. Such processes usually carry over several months, resulting high effective costs compared expected benefits. We devised an agile "community review" system allocate micro-grants fast...

The rise of major complex public health problems, such as vaccination hesitancy and access to vaccination, requires innovative, open, transdisciplinary approaches. Yet, institutional silos lack participation on the part nonacademic citizens in design solutions hamper efforts meet these challenges. Against this background, new have been explored, with participatory research, citizen science, hackathons, challenge-based approaches being applied context health.

Doctorant en biologie de synthese a l’iSSB (Institut systemique et synthetique), fondateur president du laboratoire « La Paillasse pour les biotechnologies citoyennes », Vitry-sur-Seine, France. Campus Genopole 1, 5, rue Henri Desbrueres, 91000 Evry, thomas.landrain@issb.genopole.fr garage ou DIY biology est issue d’une vieille tradition se rattache l’envie faire des sciences soi-meme, chez soi, avec moyens bord. Elle donne d’explorer la connaissance faisant sa curiosite le moteur vie...

International development and aid are often conducted through the allocation of funding determined by decisions non-locals, especially in west for those global south. In addition, such is disassociated from local expertise, therefore providing little long-term developmental impact generating distrust. This particularly true conservation, as well environmental educational programmes. We hypothesize that granting people tools necessary to develop their own projects use an applicant-driven...

Abstract Background The rise of major complex public health problems, such as vaccination hesitancy and access to vaccination, requires innovative, open transdisciplinary approaches. In spite this, institutional silos, paywalls lack participation non-academic citizens in the design solutions hamper efforts meet these challenges. Against this background, new have been explored, with participatory research, citizen science, hackathon challenge-based approaches being applied context health....

Abstract Resource allocation is essential to the selection and implementation of innovative projects in science technology. With large stakes involved concentrating fundings over a few promising projects, current “winner-take-all” models for grant applications are time-intensive endeavours that mobilise significant researcher time writing extensive project proposals, rely on availability time-saturated volunteer experts. Such processes usually carry several months, resulting high effective...

The discovery and study of a vast number regulatory RNAs in all kingdoms life over the past decades has allowed design new synthetic that can regulate gene expression vivo. Riboregulators, particular, have been used to activate or repress expression. However, accelerate scale up process, biologists require computer-assisted tools, without which riboregulator engineering will remain case-by-case process requiring expert attention. Recently, RNA circuits by evolutionary computation adapting...

Abstract Organisms have different circuitries that allow converting signal molecule levels to changes in gene expression. An important challenge synthetic biology involves the de novo design of RNA modules enabling dynamic processing live cells. This requires a scalable methodology for sensing, transmission, and actuation, which could be assembled into larger signaling networks. Here, we present biochemical strategy RNA-mediated transduction cascades able sense small molecules RNAs. We...

<ns4:p>Resource allocation is essential to selection and implementation of innovative projects in science technology. Current “winner-take-all” models for grant applications require significant researcher time writing extensive project proposals, rely on the availability a few time-saturated volunteer experts. Such processes usually carry over several months, resulting high effective costs compared expected benefits. We devised an agile “community review” system allocate micro-grants fast...

International development and aid are often conducted through the allocation of funding determined by decisions non-locals, especially in west for those global south. In addition, such is disassociated from local expertise, therefore providing little long-term developmental impact generating distrust. This particularly true conservation, as well environmental educational programmes. We hypothesise that granting people tools necessary to develop their own projects use an applicant driven...

International development and aid are often conducted through the allocation of funding determined by decisions non-locals, especially in west for those global south. In addition, such is disassociated from local expertise, therefore providing little long-term developmental impact generating distrust. This particularly true conservation, as well environmental educational programmes. We hypothesise that granting people tools necessary to develop their own projects use an applicant driven...

International development and aid are often conducted through the allocation of funding determined by decisions non-locals, especially in west for those global south. In addition, such is disassociated from local expertise, therefore providing little long-term developmental impact generating distrust. This particularly true conservation, as well environmental educational programmes. We hypothesise that granting people tools necessary to develop their own projects use an applicant driven...

<sec> <title>BACKGROUND</title> The rise of major complex public health problems, such as vaccination hesitancy and access to vaccination, requires innovative, open, transdisciplinary approaches. Yet, institutional silos lack participation on the part nonacademic citizens in design solutions hamper efforts meet these challenges. Against this background, new have been explored, with participatory research, citizen science, hackathons, challenge-based approaches being applied context health....