A5030092474- Apelin-related biomedical research
- Nuclear Receptors and Signaling
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Developmental Biology and Gene Regulation
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Connective tissue disorders research
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- Nuts composition and effects
- Bone and Dental Protein Studies
- Congenital Heart Disease Studies
- Mitochondrial Function and Pathology
- Zebrafish Biomedical Research Applications
- 14-3-3 protein interactions
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Vascular anomalies and interventions
- RNA Interference and Gene Delivery
- Hypertrophic osteoarthropathy and related conditions
- Food Quality and Safety Studies
- Hippo pathway signaling and YAP/TAZ
Agency for Science, Technology and Research
2009-2022
Institute of Medical Biology
2009-2022
Preeclampsia (PE) is a gestational hypertensive syndrome affecting between 5 and 8% of all pregnancies. Although PE the leading cause fetal maternal morbidity mortality, its molecular etiology still unclear. Here, we show that ELABELA (ELA), an endogenous ligand apelin receptor (APLNR, or APJ), circulating hormone secreted by placenta. Elabela but not Apelin knockout pregnant mice exhibit PE-like symptoms, including proteinuria elevated blood pressure due to defective placental angiogenesis....
A key step in the de novo formation of embryonic vasculature is migration endothelial precursors, angioblasts, to position future vessels. To form first axial vessels, angioblasts migrate towards midline and coalesce underneath notochord. Vascular growth factor has been proposed serve as a chemoattractant for regulate this medial migration. Here we challenge model instead demonstrate that rely on their intrinsic expression Apelin receptors (Aplr, APJ) midline. We further show during...
Formation of the three embryonic germ layers is a fundamental developmental process that initiates differentiation. How zebrafish pluripotency factor Pou5f3 (homologous to mammalian Oct4) drives lineage commitment unclear. Here, we introduce fluorescence lifetime imaging microscopy and correlation spectroscopy assess formation complexes with other transcription factors in real-time gastrulating embryos. We show, at single-cell resolution vivo, Nanog pattern mesendoderm differentiation...
The Apelin receptor (Aplnr) is essential for heart development, controlling the early migration of cardiac progenitors. Here we demonstrate that in zebrafish Aplnr modulates Nodal/TGFβ signaling, a key pathway mesendoderm induction and migration. Loss function leads to reduction Nodal target gene expression whereas activation by non-peptide agonist increases these same targets. Furthermore, loss results delay cardiogenic transcription factors mespaa/ab. Elevating levels aplnra/b morphant...