A5008099127- Telomeres, Telomerase, and Senescence
- Advanced biosensing and bioanalysis techniques
- Digestive system and related health
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Cancer Research and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Radiation Therapy and Dosimetry
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Nutrition, Genetics, and Disease
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- PARP inhibition in cancer therapy
- Renal and related cancers
- Radiopharmaceutical Chemistry and Applications
- Chromosomal and Genetic Variations
- Peptidase Inhibition and Analysis
- Neuroblastoma Research and Treatments
Geron (United States)
1994-2004
Menlo School
1994-2004
The University of Texas Southwestern Medical Center
1992-1997
The University of Texas MD Anderson Cancer Center
1997
Southwestern Medical Center
1996
The University of Texas at Austin
1995-1996
Hiroshima University
1995
McMaster University
1994-1995
University of Toronto
1995
Cold Spring Harbor Laboratory
1994
Synthesis of DNA at chromosome ends by telomerase may be necessary for indefinite proliferation human cells. A highly sensitive assay measuring activity was developed. In cultured cells representing 18 different tissues, 98 100 immortal and none 22 mortal populations were positive telomerase. Similarly, 90 101 biopsies 12 tumor types 50 normal somatic tissues positive. Normal ovaries testes positive, but benign tumors such as fibroids negative. Thus, appears to stringently repressed in...
Telomerase is a ribonucleoprotein that synthesizes telomere repeats onto chromosome ends and involved in maintaining length germline tissues immortal cancer cells. In the present study, temporal regulation of expression telomerase activity was examined human somatic cells during development. detected fetal, newborn, adult testes ovaries, but not mature spermatozoa or oocytes. Blastocysts expressed high levels as did most at 16–20 weeks development with exception brain tissue. This could no...
Abstract This is the first report describing up-regulation of telomerase activity in human normal cells. Telomerase, a ribonucleoprotein enzyme, has been thought to be involved maintaining telomere length stability germline and most cancer cells, but not However, present study, we demonstrate that detectable at low levels T B increases by vitro mitogenic stimulation, hematopoietic progenitor cells upon their proliferation differentiation, decreases with aging. Understanding regulation may...
Journal Article Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity Get access Woodring E. Wright, Wright * The University Texas Southwestern Medical Center at Dallas, Department Cell Biology Neuroscience5323 Harry Hines Boulevard, TX 75235-9039, USA *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Jerry W. Shay, Shay Mieczyslaw A. Piatyszek...
Journal Article Telomerase Activity in Small-Cell and Non–Small-Cell Lung Cancers Get access Keiko Hiyama, Hiyama Department of Cell Biology Neuroscience. The University Texas Southwestern Medical Center at Dallas Second Internal Medicine, Hiroshima School MedicineJapan Search for other works by this author on: Oxford Academic PubMed Google Scholar Eiso Neuroscience, Dallas, General Medicine Shinichi Ishioka, Ishioka Michio Yamakido, Yamakido Kouki Inai, Inai Pathology, Adi F. Gazdar, Gazdar...
Background:The activity of the ribonucleoprotein enzyme telomerase is not detected in normal somatic cells; thus, with each cell division, ends chromosomes consisting telomeric repeats TTAGGG progressively erode. The current model gaining support that germline and immortal cells maintains telomere length thus compensates for “end-replication problem.”
Individuals with germ line mutations in the p53 gene, such as Li-Fraumeni syndrome (LFS), have an increased occurrence of many types cancer, including unusually high incidence breast cancer. This report documents that normal epithelial cells obtained from a patient LFS (with mutation at codon 133 gene) spontaneously immortalized cell culture while stromal fibroblasts this same did not. Spontaneous immortalization human vitro is extremely rare event. first documented case spontaneous culture....
Telomerase is a ribonucleoprotein that synthesizes telomere repeats onto chromosome ends and involved in maintaining length germline tissues immortal cancer cells. In the present study, temporal regulation of expression telomerase activity was examined human somatic cells during development. detected fetal, newborn, adult testes ovaries, but not mature spermatozoa or oocytes. Blastocysts expressed high levels as did most at 16–20 weeks development with exception brain tissue. This could no...
AIMS/BACKGROUND: Telomerase, an enzyme associated with cellular immortality, is expressed by most malignant tumours, but inactive in normal somatic cells except for male germ and proliferating stem cells. Thus, the measurement of telomerase activity tissue samples may provide useful diagnostic prognostic information. The aim this study was to determine whether expression detection occult lymph nodes. METHODS: Telomerase compared histological findings 123 surgically removed nodes submitted...