A5048736628- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Nuts composition and effects
- Horticultural and Viticultural Research
- Plant Genetic and Mutation Studies
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Biofuel production and bioconversion
- Insect-Plant Interactions and Control
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Photosynthetic Processes and Mechanisms
- Crop Yield and Soil Fertility
- Plant Pathogens and Resistance
- Phytoplasmas and Hemiptera pathogens
- Yeasts and Rust Fungi Studies
- Plant-Microbe Interactions and Immunity
- Silicon Effects in Agriculture
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
University of California, Berkeley
2009-2024
Plant Gene Expression Center
2000-2023
United States Department of Agriculture
2007-2009
University of Missouri–St. Louis
2007
Pennsylvania State University
2007
Oregon State University
2000-2006
Agricultural Research Service
2005
Cornell University
1997
Development in higher plants depends on the activity of meristems, formative regions that continuously initiate new organs at their flanks. Meristems must maintain a balance between stem cell renewal and organ initiation. In fasciated mutants, initiation fails to keep pace with meristem proliferation. The thick tassel dwarf1 (td1) mutation maize affects both male female inflorescence development. inflorescence, which results ear, is fasciated, extra rows kernels. or tassel, shows an increase...
Abstract Organogenesis in plants is controlled by meristems. Axillary meristems, which give rise to branches and flowers, play a critical role plant architecture reproduction. Maize (Zea mays) rice (Oryza sativa) have additional types of axillary meristems the inflorescence compared Arabidopsis (Arabidopsis thaliana) thus provide an excellent model system study meristem initiation. Previously, we characterized barren inflorescence2 (bif2) mutant maize showed that bif2 plays key lateral...
Plant architecture is dictated by precise control of meristematic activity. In the shoot, an imbalance in positive or negative maintenance signals can result a fasciated enlarged meristem phenotype. ear4 (fea4) semidwarfed mutant with ears and tassels as well greatly vegetative inflorescence meristems. We identified FEA4 bZIP transcription factor, orthologous to Arabidopsis thaliana PERIANTHIA. was expressed peripheral zone shoot apical vasculature immature leaves conspicuously excluded from...
Plant architecture results from a balance of indeterminate and determinate cell fates. Cells with fates are located in meristems, comprising groups pluripotent cells that produce lateral organs. Meristematic also found intercalary stem tissue, which provides for internodes, at leaf margins to contribute width. We identified maize (Zea mays) mutant has defect balancing determinacy indeterminacy. The narrow leaves short suggesting reduction the stem. In contrast, mutants fail control...
Abstract Maize is monecious, with separate male and female inflorescences. flowers are initially bisexual but achieve sexual identities through organ arrest. Loss-of-function mutants in the jasmonic acid (JA) pathway have only due to failure abort silks tassel. Tasselseed5 ( Ts5 ) shares this phenotype dominant. Positional cloning transcriptomics of tassels identified an ectopically expressed gene CYP94B subfamily, (ZmCYP94B1) . enzymes wound inducible inactivate bioactive...
Ninety hazelnut ( Corylus sp.) genotypes were surveyed for response to the eastern filbert blight pathogen [ Anisogramma anomala (Peck) E. Müller] following greenhouse inoculation using a combination of enzyme-linked immunosorbent assay (ELISA) and visual inspection cankers. Most cultivars European avellana L.) few interspecific hybrids. Six did not display signs or symptoms disease: `Closca Molla', `Ratoli', `Yoder #5', `Potomac', `Medium Long', `Grand Traverse'. Molla' both minor Spanish...
Significance The maize ear is unbranched and terminates in a single point. tassel inflorescences of Fascicled mutants fail to grow as point instead are branched. This phenotype results from the misexpression duplicated transcription factors, ZMM8 DRL2. We hypothesize that these gene rearrangements create regulatory sequences cause early inflorescence meristems, thus activating laminar program, ablating meristem, producing branches. work demonstrates zmm8 drl2 must be restricted meristem...
The presence of mixed-linkage (1,3;1,4)-β-d-glucan (MLG) in plant cell walls is a key feature grass species such as cereals, the main source calorie intake for humans and cattle. Accumulation this polysaccharide involves coordinated regulation biosynthetic metabolic machineries. While several components MLG biosynthesis machinery have been identified diverse species, degradation poorly understood. In study, we performed large-scale forward genetic screen maize (Zea mays) mutants with altered...
In Arabidopsis, SHOOT MERISTEMLESS (STM) and CLAVATA1 (CLV1) competitively regulate meristem homeostasis. Here, we explore the interaction of their maize homologs knotted1 (kn1) thick tassel dwarf1 (td1). kn1 mutants form fewer lateral organs td1 inflorescences are fasciated with additional floral organs. Double show epistatic to in seedling ear development but dose-sensitivity exists later promote leaf initiation. Thus function a pathway maintain homeostasis products may interact different...
The development of efficient pipelines for the bioconversion grass lignocellulosic feedstocks is challenging due to limited understanding molecular mechanisms controlling synthesis, deposition, and degradation varying polymers unique cell walls. Here, we describe a large-scale forward genetic approach resulting in identification collection chemically mutagenized maize mutants with diverse alterations their wall attributes such as crystalline cellulose content or hemicellulose composition....
Eastern filbert blight (EFB), caused by the fungus Anisogramma anomala (Peck) E. Müller, is an important disease of european hazelnut ( Corylus avellana L.) in Pacific northwestern United States. In 1989, a chance seedling free EFB was discovered adjacent to severely diseased orchard near Troutdale, Ore. This selection, subsequently named `Zimmerman', crossed with three susceptible selections. Based on morphological characters and incompatibility alleles, we speculated that `Zimmerman' (S 1...
Abstract Tetraploid durum wheat Triticum turgidum subsp. cv ‘Kronos’ has extensive genetic variation resources, including a sequenced and cataloged ethyl methanesulfonate (EMS) mutagenized population. To utilize this allelic diversity, we screened over 2,000 mutant lines identified 30 enhanced disease resistance (EDR) mutants in forward field screen against stripe rust. Sixteen of the EDR have persistent to rust after four years, several showed differential responses other fungal pathogens,...
SUMMARY Narrow odd dwarf ( nod ) and Liguleless narrow Lgn are pleiotropic maize mutants that both encode plasma membrane proteins, cause similar developmental patterning defects, constitutively induce stress signaling pathways. To investigate how these coordinate development physiology, we screened for protein interactors of NOD by affinity purification. LGN was identified this screen as a strong candidate interactor, confirmed the NOD‐LGN molecular interaction through orthogonal...
Abstract Solanum habrochaites LA1353 is a self-incompatible, highly heterozygous wild tomato that useful germplasm resource for the study of metabolism, reproduction and disease resistance. We generated draft genome assembly with PacBio HiFi reads annotations, which underscored expansion gene families associated metabolite-production, self-incompatibility, DNA regulation immunity. After manually curating intracellular nucleotide-binding leucine-rich repeat immune receptors (NLRs), we found...
The presence of mixed-linkage (1,3;1,4)-β-D-glucan (MLG) in plant cell walls is a key feature grass species such as cereals - the main source calorie intake for humans and cattle. Accumulation this polysaccharide involves coordinated regulation biosynthetic metabolic machineries. While several components MLG biosynthesis machinery have been identified diverse species, degradation poorly understood. A large-scale forward genetic maize screen mutants with altered wall structural properties...
ABSTRACT narrow odd dwarf ( nod ) and Liguleless Lgn are pleiotropic maize mutants that both encode plasma membrane proteins, cause similar developmental patterning defects, constitutively induce stress signaling pathways. To investigate how these coordinate development physiology, we screened for protein interactors of NOD by affinity purification. LGN was identified this screen as a strong candidate interactor, confirmed the NOD-LGN molecular interaction through orthogonal experiments. We...
A survey of hazelnut ( Corylus avellana L.) genotypes for response to the eastern filbert blight pathogen [ Anisogramma anomala (Peck) E. Müller] was performed. Seven varieties were discovered that did not display disease signs or symptoms when subjected severe inoculation with A. in greenhouse and assayed infection. These cultivars are `Closca Molla', `Ratoli', `Yoder #5', `Potomac', `Medium Long', `Grand Traverse' `Zimmerman'. `Ratoli' both minor from Spain, superior agronomic types...