Perimenopausal period refers to the interval when women's menstrual cycles become irregular and is characterized by an increased risk of depression. Use homeopathy treat depression widespread but there a lack clinical trials about its efficacy in peri- postmenopausal women. The aim this study was assess safety individualized homeopathic treatment versus placebo fluoxetine women with moderate severe depression.A randomized, placebo-controlled, double-blind, double-dummy, superiority,...

To identify the relationship between landscape use and dengue virus (DENV) occurrence in bats, we investigated presence of DENV from anthropogenically changed unaltered landscapes two Biosphere Reserves: Calakmul (Campeche) Montes Azules (Chiapas) southern Mexico. Spleen samples 146 belonging to 16 species, were tested for four serotypes with standard reverse transcriptase polymerase chain reaction (RT-PCR) protocols. Six bats (4.1%) positive DENV-2: (two Glossophaga soricina , one Artibeus...

Dengue (DENV) and Chikungunya (CHIKV) viruses can be transmitted simultaneously by Aedes mosquitoes, there may co-infections in humans. However, how the adaptive immune response is modified host has yet to known entirely. In this study, we analyzed cross-reactivity neutralizing activity of IgG antibodies against DENV CHIKV sera patients from Mexican Institute Social Security Veracruz, Mexico, collected 2013 2015 using BALB/c mice inoculated with and/or CHIKV. Mice first then produced that...

This study describes, to some extent, the VCC contribution as an early stimulation of macrophage lineage. Regarding onset innate immune response caused by infection, β form IL-1 is most important interleukin involved in inflammatory response. Activated macrophages treated vitro with induced activation MAPK signaling pathway a one-hour period, transcriptional regulators for surviving and pro-inflammatory response, suggesting explanation inspired supported inflammasome physiology. The...

Climacteric is associated with both depression and metabolic dysregulation. Scarce evidence suggests that dysregulation may predict poor response to conventional antidepressants. Response treatment has not been studied in homeopathic medicine. The aim of this study was investigate the prevalence disorders depressed climacteric women treated medicines, fluoxetine or placebo, if these alterations have any association treatment. One hundred thirty-three Mexican (40–65 years) depression,...

N-ω-chloroacetyl-L-ornithine (NCAO) is an ornithine decarboxylase (ODC) inhibitor that known to exert cytotoxic and antiproliferative effects on three neoplastic human cancer cell lines (HeLa, MCF-7, HepG2). Here, we show NCAO has activity in 13 lines, of diverse tissue origin from mice, a mouse model vivo. All were sensitive after 72 h treatment (the EC50 ranged 1 50.6 µmol/l). The Ca Ski line was the most (EC50=1.18±0.07 µmol/l) MDA-MB-231 least (EC50=50.6±0.3 This ODC showed selectivity...

No previous studies have investigated the use of a premixed insulin analogue in hospital setting.To compare efficacy and safety treatment with (insulin lispro mix 75/25, LM75/25) basal-plus regimen glargine hospitalized patients type 2 diabetes (T2D).A randomized clinical trial T2D glucose >140 mg/dL on admission was performed. A total 54 were to receive LM75/25 or glargine. In both groups, correction dose administered before meals. Insulin adjusted obtain mean blood (BG) between 100 140...

Many cancer cells have high expression of ornithine decarboxylase (ODC) and there is a concerted effort to seek new inhibitors this enzyme. The aim the study was initially characterize inhibition properties, then evaluate cytotoxicity/antiproliferative cell based activity N-ω-chloroacetyl-l-ornithine (NCAO) on three human lines. Results showed NCAO be reversible competitive ODC inhibitor (Ki = 59 µM) with cytotoxic antiproliferative effects, which were concentration- time-dependent. EC50,72h...

Background Although individualized homeopathic treatment is effective for depression in climacteric women, there a lack of well-designed studies its efficacy battered women or post-traumatic stress disorder. The aim this study was to assess the association between fluoxetine and response with high levels domestic violence, sexual abuse marital dissatisfaction. Materials Methods One hundred thirty-three Mexican moderate-to-severe enrolled HOMDEP-MENOP Study (a randomized, placebo-controlled,...

Background . Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions elution of the sample need to be optimized each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized validated protocol specific detection dengue antibodies from dried spots (DSSs). Methods Paired DSS 66 suspected cases were collected clinic Veracruz, Mexico. Samples sent our laboratory, where optimal DSSs...

Heterologous secondary infections are at increased risk of developing dengue hemorrhagic fever (DHF) because antibody-dependent enhancement (ADE). IgG subclasses can fix and activate complement bind to Fcɣ receptors. These factors may also play an important role in the development ADE thus pathogenesis DHF. The aim this study was analyze indices anti-dengue adult patients with febrile acute phase. In 2013, 129 (DF) 57 DHF Veracruz, Mexico were recruited for IgM determined by capture ELISA....

Background: The Geranium genus is taxonomically classified within the family Geraniaceae Juss, which includes 5-11 genera and nearly 750species in total. best-known of this are Geranium, consisting largely wild plants, Pelargonium, ofornamental plants. Traditional uses include as an antiseptic wounds antipyretic by infusion plant.Methods: This paper summarized previous recent reports hepato-protective activities used traditional medicine.Results: Currently, eight different species geraniums...

Spirulina (formerly Arthrospira) maxima (SP) is a cyanobacterium reported to have great nutritional and pharmacological potential. The objective of this study was evaluate the protective properties SP against ethanol-induced toxicity. Male Wistar rats were used in subjected 70% partial hepatectomy (PH); they then divided into five groups. During experiment, animals two groups drank an aqueous solution ethanol (EtOH) (40%, v/v). Additionally, administered extract daily at dose 200 mg/kg body...

Dengue manifestations range from a mild form, dengue fever (DF), to more severe forms such as hemorrhagic (DHF) and shock syndrome (DSS). The ability of the host present one these clinical could be related polymorphisms located in genes Toll-like receptors (TLRs) which activate pro-inflammatory response. Therefore, genotyping single nucleotide genetic (SNPs) TLR3 (rs3775291 rs6552950), TLR4 (rs2737190, rs10759932, rs4986790, rs4986791, rs11536865, rs10983755), TLR7 (rs179008 rs3853839), TLR8...

West Nile virus (WNV) emerged in the Americas with its introduction 1999 and now is considered endemic across continent. In 2002, WNV was detected Mexico, where occurrence mortality are considerably lower compared US. However, continuous national surveillance programs Mexico nonexistent. Birds primary hosts geographic dispersers of this pathogen. A total 200 cloacal tracheal samples from wild migratory or resident birds were retrospectively analyzed using reverse transcription PCR to detect...

The perimenopausal period refers to the interval when women’s menstrual cycles become irregular and is characterized by an increased risk of depressive symptoms. Use homeopathy treat depression widespread but there a lack clinical trials about its efficacy in peri- postmenopausal women. Previous suggest that individualized homeopathic treatments improve depression. In classical homeopathy, individually selected remedy prescribed after complete case history patient. aim this study assess...

Background Polyamines are involved in several cellular processes and inhibiting their synthesis affects chikungunya virus (CHIKV) replication translation, and, therefore, reduces the quantity of infectious viral particles produced. In this study, we evaluated inhibition CHIKV by N-ω-chloroacetyl-L-ornithine (NCAO), a competitive inhibitor ornithine decarboxylase, an enzyme which is key biosynthesis polyamines (PAs). Methods The cytotoxicity NCAO was MTT cell culture. inhibitory effect Vero...

Chikungunya virus (CHIKV) is classified as a pathogen with the potential to cause pandemic. This situation becomes more alarming since no approved drug exists combat virus. The present research aims demonstrate anti-CHIKV activity of molecules in latex Euphorbia grandicornis. Therefore, biodirected assay was carried out find activity. Extractions hexane, dichloromethane, and methanol subsequent purification by column chromatography were later evaluate cytotoxic...

Information is scarce about the presence of molecular alterations related to human papillomavirus (HPV) infection in squamous cell carcinomas genital skin and effect this number Langerhans cells present these tumors.To determine HPV see relationship between changes expression Ki-67 antigen (Ki-67), p53 protein (p53), retinoblastoma (pRb) E-cadherin density, if any.A descriptive, comparative, retrospective cross-sectional study was performed with all cases diagnosed as at Dermatopathology...