A5067988831- Genetic Neurodegenerative Diseases
- Pluripotent Stem Cells Research
- Neurological disorders and treatments
- Heat shock proteins research
- Ultrasound and Hyperthermia Applications
- Nerve injury and regeneration
- Parkinson's Disease Mechanisms and Treatments
- Autism Spectrum Disorder Research
- Ultrasound Imaging and Elastography
- Ultrasound and Cavitation Phenomena
- Photoacoustic and Ultrasonic Imaging
- Neurogenesis and neuroplasticity mechanisms
- Mitochondrial Function and Pathology
- CAR-T cell therapy research
- Neuroscience and Neural Engineering
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- Muscle Physiology and Disorders
- Biochemical effects in animals
- Xenotransplantation and immune response
- Cell death mechanisms and regulation
- Alzheimer's disease research and treatments
- Neuropeptides and Animal Physiology
- Cytomegalovirus and herpesvirus research
- Cholesterol and Lipid Metabolism
Centre National de la Recherche Scientifique
2012-2024
Université Paris-Saclay
2018-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2024
Université Paris-Sud
2018-2024
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2012-2024
CEA Paris-Saclay
2018-2024
Direction de la Recherche Fondamentale
2019-2024
Inserm
2018
Institut de Radiobiologie Cellulaire et Moléculaire
2018
Service de la Santé Publique
2016
Focused ultrasound (FUS) in combination with microbubbles is capable of noninvasive, site-targeted delivery drugs through the blood–brain barrier (BBB). Although acoustic parameters are reproducible small animals, their control remains challenging primates due to skull heterogeneity. This study describes a 7-T magnetic resonance (MR)-guided FUS system designed for BBB disruption non-human (NHP) robust feedback based on passive cavitation detection (PCD). Contrast enhanced T 1 -weighted MR...
Abstract Cell therapy products (CTP) derived from pluripotent stem cells (iPSCs) may constitute a renewable, specifically differentiated source of to potentially cure patients with neurodegenerative disorders. However, the immunogenicity CTP remains major issue for therapeutic approaches based on transplantation non-autologous cell-derived neural grafts. Despite its considerable side-effects, long-term immunosuppression, appears indispensable mitigate neuro-inflammation and prevent rejection...
Abstract Ultrasound-induced blood-brain barrier (BBB) opening using microbubbles is a promising technique for local delivery of therapeutic molecules into the brain. The real-time control ultrasound dose delivered through skull necessary as range pressure efficient and safe BBB very narrow. Passive cavitation detection (PCD) method proposed to monitor microbubble activity during exposure. However, there still no consensus on reliable safety indicator able predict potential damage in Current...
Heat shock protein 27 (HSP27) has a major role in mediating survival responses to range of central nervous system insults, functioning as chaperone, an antioxidant, and through inhibition cell death pathways. We have used transgenic mice overexpressing HSP27 (HSP27tg) examine the cerebral ischemia, using model permanent middle artery occlusion (MCAO). Infarct size was evaluated multislice T 2 -weighted anatomical magnetic resonance imaging (MRI) after 24 h. A significant reduction 30%...
Neural transplantation is a promising therapeutic approach for neurodegenerative diseases; however, many patients receiving intracerebral fetal allografts exhibit signs of immunization to donor antigens that could compromise the graft. In this context, we intracerebrally transplanted mesencephalic pig xenografts into primates identify suitable strategy enable long-term cell survival, maturation, and differentiation. Parkinsonian received WT or CTLA4-Ig transgenic porcine different durations...
Abstract There has been substantial progress in the development of regenerative medicine strategies for CNS disorders over last decade, with progression to early clinical studies some conditions. However, there are multiple challenges along translational pipeline, many which common across diseases and pertinent donor cell types. These include defining point at preclinical data sufficiently compelling permit first studies; scaling-up, characterization, quality control validation product;...
Heat shock proteins (HSPs) are molecular chaperones with essential roles in modulating the proteolytic machinery and accelerating cell repair. protein overexpression has been observed vivo vitro under stresses including heat, nutrient deprivation ischemia. Experiments models of stroke indicate that transgenically overexpressed or virally delivered HSPs can enhance survival, but cannot always reduce lesion size. This study aims to assess effects a rat middle cerebral artery occlusion model...
The role of alpha-synuclein in Parkinson's disease has been heavily investigated since its discovery as a component Lewy bodies. Recent rodent data demonstrate that strain structure is critical for differential propagation and toxicity. Based on these findings, we have compared, the first time, this pilot study, capacity two strains patient-derived body extracts to model synucleinopathies after intra-putaminal injection non-human primate brain. Functional alterations triggered by injections...
A recent phase I-II, open-label trial of ProSavin, a lentiviral vector delivering the key enzymes in dopamine biosynthetic pathway to non-dopaminergic striatal neurons, demonstrated safety and improved motor function parkinsonian patients. However, magnitude effect suggested that optimal levels replacement may not have been achieved. OXB-102, with an optimized expression cassette for biosynthesis, has shown achieve significantly higher yield than ProSavin. We assessed efficacy OXB-102 MPTP...
Heat shock proteins (HSPs) function as molecular chaperones involved in protein folding, transport and degradation and, addition, they can promote cell survival both vitro vivo after a range of stresses. Although some studies have suggested that HSP27 HSP70 be neuroprotective, current evidence is limited, particularly when HSPs been delivered an insult. The effect overexpressing transient occlusion the middle cerebral artery rats was investigated by delivering attenuated herpes simplex viral...
The development of dyskinesias following chronic L-DOPA replacement therapy remains a major problem in the long-term treatment Parkinson’s disease. This study aimed at evaluating effect IRC-082451 (base BN82451), novel multitargeting hybrid molecule, on L-DOPA-induced (LIDs) and hypolocomotor activity non-human primate model PD. displays multiple properties: it inhibits neuronal excitotoxicity (sodium channel blocker), oxidative stress (antioxidant) neuroinflammation (cyclooxygenase...
OBJECTIVE Deep brain stimulation (DBS) of the subthalamic nucleus (STN) is a well-established therapy for motor symptoms in patients with pharmacoresistant Parkinson's disease (PD). However, procedure, which requires multimodal perioperative exploration such as imaging, electrophysiology, or clinical examination during macrostimulation to secure lead positioning, remains challenging because STN cannot be reliably visualized using gold standard, T2-weighted imaging (T2WI) at 1.5 T. Thus,...
Posttransplant lymphoproliferative disorders (PTLDs) are a heterogeneous group of lymphoid proliferations that occur in the setting depressed T-cell function due to immunosuppressive therapy used following solid organ transplantation, hematopoietic stem cell and also xenotransplantation. In present study, 28 immunosuppressed parkinsonian Macaca fascicularis were intracerebrally injected with wild-type or CTLA4-Ig transgenic porcine xenografts identify suitable strategy enable long-term...
The 18 kDa translocator protein (TSPO) is the main molecular target to image neuroinflammation by positron emission tomography (PET). However, TSPO-PET quantification complex and none of kinetic modelling approaches has been validated using a voxel-by-voxel comparison data with actual TSPO levels expression. Here, we present single case study binary classification in vivo PET evaluate statistical performance different methods. To that end, induced localized adjustable increase non-human...