A5102834790- Monoclonal and Polyclonal Antibodies Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Systemic Lupus Erythematosus Research
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- T-cell and B-cell Immunology
- Adipose Tissue and Metabolism
- Retinal Development and Disorders
- bioluminescence and chemiluminescence research
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Vasculitis and related conditions
- Retinal Diseases and Treatments
- Coenzyme Q10 studies and effects
- Peroxisome Proliferator-Activated Receptors
- Nicotinic Acetylcholine Receptors Study
- Glaucoma and retinal disorders
- Glycosylation and Glycoproteins Research
- Biochemical Acid Research Studies
- Advanced battery technologies research
- Alcohol Consumption and Health Effects
- Toxin Mechanisms and Immunotoxins
University of Tennessee Health Science Center
2016-2019
Karolinska Institutet
2018-2019
Karolinska University Hospital
2018-2019
B cells are postulated to be central in seropositive rheumatoid arthritis (RA). Here, we use exploratory mass cytometry (n = 23) and next-generation sequencing 19) study B-cell repertoire shifts RA patients. Expression of several markers were significantly different ACPA+ compared healthy controls, including an increase HLA-DR across subsets, CD22 clusters IgM+ CD11c IgA+ memory. Moreover, both IgG+ double negative (IgD- CD27-) CD11c+ increased RA, there was a trend for elevation...
Rheumatoid arthritis (RA) associated anti-citrullinated protein autoantibodies (ACPA) target a wide range of modified proteins. Citrullination occurs during physiological processes such as apoptosis, yet little is known about the interaction ACPA with nuclear antigens or apoptotic cells. Since uncleared cells and neutrophil extracellular trap (NET) products have been postulated to be central sources autoantigen immunostimulation in autoimmune disease, we sought characterize anti-nuclear...
Autoreactive B cells have a central role in the pathogenesis of rheumatoid arthritis (RA), and recent findings proposed that anti-citrullinated protein autoantibodies (ACPA) may be directly pathogenic. Herein, we demonstrate frequency variable-region glycosylation single-cell cloned mAbs. A total 14 ACPA mAbs were evaluated for predicted N-linked motifs silico compared to 452 highly-mutated from RA patients controls. Variable region (N-X-S/T) strikingly prevalent within (100%) somatically...
Alcohol consumption affects human health in part by compromising the immune system. In this study, we examined expression of Cd14 (cluster differentiation 14) gene, which is involved system through a proinflammatory cascade. Expression was evaluated BXD mice treated with saline or acute 1.8 g/kg i.p. ethanol (12.5% v/v). Hippocampal gene data were generated to examine differential and perform systems genetics analyses. The showed significant changes among strains after treatment, eQTL...
Coenzyme Q (CoQ) is a well-studied molecule, present in every cell membrane the body, best known for its roles as mitochondrial electron transporter and potent anti-oxidant. Much of previous work was done vitro yeast more recent has suggested that CoQ may have additional prompting calls re-assessment role using vivo systems mammals. Here we investigated putative ethanol-induced effects BXD RI mice. We examined hippocampal expression Coq7 saline controls after an acute ethanol treatment,...
<h3>Career situation of first and presenting author</h3> Young investigator. <h3>Introduction</h3> Anti-citrullinated protein autoantibodies (ACPA) in RA patients target a wide range modified proteins recent findings reveal that monoclonal ACPA are cross-reactive due to recognition shared consensus citrulline-peptide motifs. Among physiological targets ACPA, citrullination neutrophil extracellular trap (NET) products, have been postulated be important. <h3>Objectives</h3> We sought...
<h3>Background:</h3> Seropositive rheumatoid arthritis (RA) is associated with autoreactivity to citrullinated proteins and factor activity. The adaptive immune system B cells are postulated be central in RA pathogenesis, yet possible B-cell dysregulations have not been extensively studied. Here, we use exploratory mass cytometry next generation sequencing study overall cell repertoire shifts patients compared healthy individuals. <h3>Objectives:</h3> We hypothesized that there an underlying...