A5086385270- Adrenal Hormones and Disorders
- Hormonal Regulation and Hypertension
- Hormonal and reproductive studies
- Pituitary Gland Disorders and Treatments
- Adrenal and Paraganglionic Tumors
- Stress Responses and Cortisol
- Vitamin D Research Studies
- Thyroid Disorders and Treatments
- Pharmacological Effects of Natural Compounds
- Sexual Differentiation and Disorders
- Hemodynamic Monitoring and Therapy
- Dental Health and Care Utilization
- Growth Hormone and Insulin-like Growth Factors
- Electrolyte and hormonal disorders
- Oral microbiology and periodontitis research
- Ophthalmology and Eye Disorders
- Tryptophan and brain disorders
- Adipose Tissue and Metabolism
- Thyroid and Parathyroid Surgery
- Metabolism and Genetic Disorders
- Cardiac, Anesthesia and Surgical Outcomes
- Pharmacological Effects and Assays
- Occupational Health and Performance
- Inhalation and Respiratory Drug Delivery
- Sinusitis and nasal conditions
University of Bergen
2016-2025
Haukeland University Hospital
2014-2024
Fundação Faculdade de Odontologia
2023
Akershus University Hospital
2014
Diabetes Care & Hormone Clinic
2011
Rhythms are intrinsic to endocrine systems, and disruption of these hormone oscillations occurs at very early stages the disease. Because adrenal hormones secreted with both circadian ultradian periods, conventional single–time point measurements provide limited information about rhythmicity and, crucially, do not during sleep, when many fluctuate from nadir peak concentrations. If blood sampling is attempted overnight, then this necessitates admission a clinical research unit, can be...
Conventional glucocorticoid replacement therapy fails to mimic the physiological cortisol rhythm, which may have implications for morbidity and mortality in patients with Addison's disease.The objective of study was compare effects continuous sc hydrocortisone infusion (CSHI) conventional oral (OHC) therapy.This a prospective crossover, randomized, multicenter clinical trial comparing 3 months treatment thrice-daily OHC vs CSHI. From Norway Sweden, 33 were enrolled from registries clinics....
Cortisol is released upon activation of the hypothalamic–pituitary–adrenal axis, varies across day, possesses an underlying diurnal rhythm and responsive to stressors. The endogenous circadian peak cortisol occurs in morning, increases observed post-awakening have been named awakening response (CAR) based on belief that act waking up stimulates secretion. However, objective evidence induces secretion limited. We used a mixed effects model with linear spline fitted data examine tissue-free...
Liquid chromatography-mass spectrometry (LC-MS/MS) offers superior analytical specificity compared to immunoassays, but is not available in many regions and hospital due expensive instrumentation tedious sample preparation. Thus, we developed an automated, high-throughput LC-MS/MS assay for simultaneous quantification of 10 endogenous synthetic steroids targeting diseases the hypothalamo-pituitary-adrenal-axis gonads.Deuterated internal standards were added 85µl serum processed by...
The overnight dexamethasone (DXM) suppression test (DST) has high sensitivity, but moderate specificity, for diagnosing hypercortisolism. We have evaluated if simultaneous measurement of S-DXM may correct variable DXM bioavailability and increase the diagnostic performance DST, saliva (sa) is a feasible adjunct or alternative to serum.Prospective study DST was carried out in patients with suspected Cushing's syndrome (CS) (n = 49), incidentaloma 152) healthy controls 101). Cortisol,...
Objective Because we found higher serum 25-hydroxyvitamin D (25(OH)D) levels among smokers than non-smokers with analyses using an electrochemiluminescence immunoassay (ECLIA) from Roche, the purpose of present study was to examine whether this difference between and maintained other 25(OH)D assays. Design A cross-sectional population-based on 6932 participants Tromsø study, 1994–1995, one validation comparing six different assays in 53 54 were performed. Methods The association smoking,...
The cosyntropin test is used to diagnose adrenal insufficiency (AI) and nonclassical congenital hyperplasia (NCCAH). Current cutoffs for cortisol 17-hydroxyprogesterone (17-OHP) are derived from nonstandardized immunoassays. Liquid chromatography tandem mass spectrometry (LC-MS/MS) offers direct measurement of steroids, prompting the need re-establish normal ranges. goal this study was define cutoff values 17-OHP in serum by LC-MS/MS 30 60 minutes after intravenous administration 250 µg...
Article Abstract Objective: The hypothalamic-pituitary-adrenal (HPA) axis seems dysregulated and part of the pathophysiology in bipolar disorder schizophrenia, but underlying mechanisms are unknown. Recent evidence indicates that systemic cortisol metabolism influences blood levels HPA functioning. Our objective was to estimate by means activity 5α-reductase, 5β-reductase, 11β-hydroxysteroid dehydrogenase (11β-HSD) patients with schizophrenia spectrum disorders compared healthy controls....
Autonomous cortisol secretion (ACS) can be unilateral or bilateral irrespective of the presence an adrenal tumor. A reliable method to distinguish between and ACS is lacking. Evaluate use venous sampling (AVS) ACS. This was a prospective study AVS in patients with tumors who received diagnosis Cushing syndrome (CS). Unilateral defined as >2.3-fold difference levels two veins. Metanephrine were used ascertain correct catheter position. Results correlated findings on CT iodine-131-cholesterol...
<title>Abstract</title> Primary aldosteronism (PA) affects 5–20% of the hypertensive population and is associated with increased cardiovascular metabolic risks compared primary hypertension. PA significantly underdiagnosed partly due to our inability capture pulsatile variability aldosterone over 24 hours in clinical settings. To gain insight into steroid secretion patterns we applied multiplex 24-hour profiling free corticosteroids concentrations subcutaneous tissue microdialysate 60...
Failure to mirror the diurnal cortisol profile could contribute impaired subjective health status in Addison's disease (AD). Some patients report benefit from use of various nutritional compounds. The objective this study was investigate impact licorice and grapefruit juice (GFJ) on absorption metabolism cortisone acetate (CA).Patients (n=17) with AD stable CA replacement therapy were recruited outpatient clinic at Haukeland University Hospital, Norway. They assessed their ordinary...
Conventional glucocorticoid replacement therapy in patients with Addison's disease (AD) is unphysiological possible adverse effects on mortality, morbidity and quality of life. The diurnal cortisol profile can likely be restored by continuous subcutaneous hydrocortisone infusion (CSHI).The aim this study was to compare circadian hormone rhythms insulin sensitivity conventional thrice-daily regimen CSHI treatment AD.An open, randomized, two-period, 12-week crossover multicentre trial Norway...
ObjectiveTo define liquid chromatography tandem mass spectrometry (LC-MS/MS)–based cutoff levels and panels of steroid hormones, to improve diagnosis nonclassic congenital adrenal hyperplasia (NCCAH) other partial enzyme defects in the adrenals.DesignProspective cohort analysis.SettingUniversity hospital-based tertiary endocrine center.PatientsOne hundred twenty-one healthy adults 65 patients evaluated for possible NCCAH (validation cohort).InterventionsThe LC-MS/MS–determined cutoffs 11...
Abstract Context Contrary to current dogma, growing evidence suggests that some patients with autoimmune Addison disease (AAD) produce corticosteroids even years after diagnosis. Objective To determine frequencies and clinical features of residual corticosteroid production in AAD. Design Two-staged, cross-sectional study 17 centers (Norway, Sweden, Germany). Residual glucocorticoid (GC) was defined as quantifiable serum cortisol 11-deoxycortisol mineralocorticoid (MC) aldosterone...
Autonomous cortisol secretion (ACS) is a condition with ACTH-independent overproduction from adrenal incidentalomas (AI) or hyperplasia. The hypercortisolism often mild, and most patients lack typical clinical features of overt Cushing's syndrome (CS). ACS not well defined diagnostic tests validation.Retrospective study 165 AI evaluated clinically by assay morning plasma ACTH, late-night saliva cortisol, serum DHEA sulphate (DHEAS), 24-h urine-free after dexamethasone suppression.Patients (n...
Objective Many patients with primary adrenal insufficiency (Addison's disease) take extra doses of glucocorticoids during stressful events, but a benefit has not been demonstrated in controlled trials. Here, we investigated the effects pre-exercise hydrocortisone dose on cardiorespiratory, hormonal and metabolic parameters response to short-term strenuous physical activity. Design This was randomized placebo-controlled, cross-over clinical trial. Participants Ten women Addison's disease 10...
Epidemiological studies support an association between obesity, metabolic syndrome (MetS), and periodontitis. Still, understanding of the effects low-grade inflammation in obese subjects on periodontitis influence MetS remains incomplete. The aims this cross-sectional study were to explore obesity related variables periodontitis, assess if is a risk indicator for sample adults.The comprised 52 adults with body mass index (BMI) ≥ 30 kg/m2 referred therapy at Obesity Centre Haukeland...
Increased intra-adipose cortisol is thought to promote obesity, but few human studies have investigated glucocorticoid hormones and none demonstrated prospective changes with fat loss.Subcutaneous adipose tissue (SAT) was obtained from obese subjects before 1-year after surgery-induced loss, nonobese controls. In a second similar cohort of subjects, adipocytes stromal-vascular fraction were isolated. Intra-adipose cortisone levels analyzed by liquid chromatography mass spectrometry...
Abstract Background No reliable biomarkers exist to guide glucocorticoid (GC) replacement treatment in autoimmune Addison’s disease (AAD), leading overtreatment with alarming and persistent side effects or undertreatment, which could be fatal. Objective To explore changes gene expression following different GC doses as a means of identifying candidate transcriptional AAD. Methods Step 1: Global microarray analysis on RNA from whole blood before after intravenous infusion 100 mg...
Abstract Background Patients with adrenal insufficiency (AI) require life-long glucocorticoid (GC) replacement therapy. Within tissues, cortisol (F) availability is under the control of isozymes 11β-hydroxysteroid dehydrogenase (11β-HSD). We hypothesize that corticosteroid metabolism altered in patients AI because nonphysiological pattern current immediate release hydrocortisone (IR-HC) The use a once-daily dual-release (DR-HC) preparation, (Plenadren®), offers more physiological profile and...
In about 95% of cases, congenital adrenal hyperplasia (CAH) is caused by mutations in CYP21A2 gene encoding steroid 21-hydroxylase (21OH). Recently, we have reported four novel variants the Norwegian population patients with CAH, which p.L388R and p.E140K were associated salt wasting (SW), p.P45L simple virilising (SV) p.V211M+p.V281L SV to non-classical (NC) phenotypes. We aimed characterise functionally utilising a newly designed vitro assay 21OH enzyme activity structural simulations...
Increased prevalence of cardiovascular disease has been reported in autoimmune Addison's (AAD), but pathomechanisms are poorly understood.Cross-sectional study.We compared serum levels 177 and inflammatory biomarkers 43 patients with AAD at >18-h glucocorticoid withdrawal matched controls, overall stratified for sex. Biomarker were correlated the frequency adrenal crises quality life (QoL) by AddiQoL-30. Finally, we investigated changes biomarker following 250 µg tetracosactide injection...