A5090947755- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Epilepsy research and treatment
- Adenosine and Purinergic Signaling
- Cannabis and Cannabinoid Research
- Forensic Toxicology and Drug Analysis
- Psychedelics and Drug Studies
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Metabolism and Genetic Disorders
- Tuberous Sclerosis Complex Research
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Histiocytic Disorders and Treatments
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Polyomavirus and related diseases
- Memory and Neural Mechanisms
- Single-cell and spatial transcriptomics
- Neurological Disorders and Treatments
Czech Academy of Sciences
2018-2023
National Institute of Mental Health
2016-2023
Czech Academy of Sciences, Institute of Physiology
2018-2023
Charles University
2016-2018
University of Chemistry and Technology, Prague
2017
Methylone (3,4-methylenedioxy-N-methylcathinone) is a synthetic cathinone analog of the recreational drug ecstasy. Although it marketed to users as relatively safe, fatalities due hyperthermia, serotonin syndrome, and multi-organ system failure have been reported. Since psychopharmacological data remain scarce, we focused our research on pharmacokinetics, detailed evaluation temporal effects methylone its metabolite nor-methylone behavior body temperature in rats. [5, 10, 20, 40 mg/kg...
The role of NMDA receptors in learning, memory and hippocampal function has long been recognized. Post-mortem studies have indicated that the expression or subunit composition glutamate receptor subtype might be related to impaired cognitive functions found schizophrenia patients. antagonists used develop animal models this disorder. There is accumulating evidence showing not only acute but also chronic application may induce schizophrenia-like alterations behavior brain functions. However,...
Mephedrone (MEPH) is a synthetic cathinone derivative with effects that mimic MDMA and/or cocaine. Our study in male Wistar rats provides detailed investigations of MEPH's and its primary metabolite nor-mephedrone's (nor-MEPH) pharmacokinetics bio-distribution to four different substrates (serum, brain, lungs liver), as well comparative analysis their on locomotion (open field test, OFT) sensorimotor gating (prepulse inhibition acoustic startle reaction, PPI ASR). Further, order the crowded...
Synthetic cannabinoid compounds are marketed as "legal" marijuana substitutes, even though little is known about their behavioral effects in relation to pharmacokinetic profiles. Therefore, the present study we assessed of systemic treatment with two synthetic cannabinoids JWH-073 and JWH-210 phytocannabinoid Δ9-THC on locomotor activity, anxiety-like phenotype (in open field) sensorimotor gating (measured prepulse inhibition acoustic startle response, PPI), serum levels. Wistar rats were...
In spite of use cannabidiol (CBD), a non-psychoactive cannabinoid, in pediatric patients with epilepsy, preclinical studies on its effects immature animals are very limited. the present study we investigated anti-seizure activity CBD (10 and 60 mg/kg administered intraperitoneally) two models chemically induced seizures infantile (12-days old) rats. Seizures were either pentylenetetrazol (PTZ) or N-methyl-D-aspartate (NMDA). parallel, brain plasma levels possible motor adverse assessed...
Objective: The adenosinergic system may influence excitability in the brain. Endogenous and exogenous adenosine has anticonvulsant activity presumably by activating A1 receptors. Adenosine receptor agonist 2-chloro-N6-cyclopentyladenosine (CCPA) thus bolster effects, but its action number of receptors at different developmental stages are not known. Methods: Hippocampal epileptic afterdischarges (ADs) were elicited 12-, 15-, 18-, 25-, 45-, 60-day-old rats. Stimulation recording electrodes...
LiCl/pilocarpine status epilepticus (SE) induced in immature rats leads, after a latent period, to hippocampal hyperexcitability. The excitability may be influenced by adenosine, which exhibits anticonvulsant activity. concentration of adenosine is regulated kinase (ADK) present two isoforms—ADK-L and ADK-S. main goal the study elucidate changes ADK isoform expression SE whether potential changes, as well inhibition 5-iodotubercidin (5-ITU), contribute during brain development. was elicited...
Background: CLARITY is a method of rendering postmortem brain tissue transparent using acrylamide-based hydrogels so that this could be further used for immunohistochemistry, molecular biology, or gross anatomical studies. Published papers the have included studies on human brains suffering from Alzheimer’s disease mouse spinal cords as animal models multiple sclerosis. Methods: We modified original design Chung system by altering electrophoretic flow-through cell, shape platinum...
Background: Postictal refractoriness, i.e., the inability to elicit a new epileptic seizure immediately after first one, is present in mature animals. Immature rats did not exhibit this and it replaced by postictal potentiation. In addition immediate potentiation, there delayed potentiation at both ages. These phenomena were studied using cortical afterdischarges as model. Objective: We aimed analyze participation of adenosine A1 receptors depression. Methods: Adenosine means Western...