Endothelial cells dysfunction has been reported in many heart diseases including acute myocardial infarction, and atherosclerosis. The molecular mechanism for endothelial the is still not clearly understood. We aimed to study role of m6A RNA demethylase alkB homolog 5 (ALKBH5) ECs angiogenesis during ischemic injury.ECs were treated with insults (lipopolysaccharide 1% hypoxia) determine ALKBH5 angiogenesis. siRNA mediated gene silencing was used examining loss function. In this study, we...

Heart failure (HF) is a leading cause of death worldwide. We have shown that pressure overload (PO)-induced inflammatory cell recruitment leads to heart in IL-10 knockout (KO) mice. However, it's unclear if PO-induced cells also target the gut mucosa, causing dysbiosis and leakage. hypothesized TAC (transverse aortic constriction) exacerbates immune homing (small intestine colon), promoting leakage KO HF was induced 8-10 weeks old C57BL/6J wild-type (WT) B6.129P2-Il10tm1Cgn/J mutant (IL-10...

A Subset of patients with irritable bowel syndrome (IBS) may have mild inflammation due to immune activation. Toll-like receptors (TLRs) and cytokines cause intestinal inflammation. We studied their expression in relation gut microbiota.Expression TLRs was assessed 47 IBS (Rome III) 25 controls using quantitative real-time polymerase chain reaction. Immunohistochemistry further performed confirm the TLR-4 TLR-5.Of IBS, 20 had constipation (IBS-C), diarrhea (IBS-D), 7 unclassified (IBS-U)....

Background: Endothelial cells (ECs) play a critical role in the maintenance of vascular homeostasis and heart function. It was shown that activated fibroblast-derived exosomes impair cardiomyocyte function hypertrophic heart, but their effect on ECs is not yet clear. Thus, we hypothesized cardiac (FB-Exo) mediate EC dysfunction, therefore modulation FB-exosomal contents may improve endothelial Methods Results: Exosomes were isolated from fibroblast (FB)-conditioned media characterized by...

Abstract Cardiac fibrosis is the hallmark of cardiovascular disease (CVD), which leading cause death worldwide. Previously, we have shown that interleukin‐10 (IL10) reduces pressure overload (PO)‐induced cardiac by inhibiting recruitment bone marrow fibroblast progenitor cells (FPCs) to heart. However, precise mechanism FPC involvement in remains unclear. Recently, exosomes and small extracellular vesicles (sEVs) been linked CVD progression. Thus, hypothesized pro‐fibrotic miRNAs enriched...

The diagnosis of cryptosporidiosis among HIV positive patients has been the focus many studies worldwide. However, there is a paucity data on negative immunocompromised like post-renal transplant recipients and those with haematological malignancies. Stool microscopy, conventional method diagnosis, fraught difficulties cumbersome sample processing subjective interpretation. Enzyme linked immunosorbent assay (ELISA), other hand, quicker easier. present study was conducted in tertiary care...

Purpose: Enteric parasitic infestation is a major public health problem in developing countries. Parasites such as Cryptosporidium spp., Cyclospora Cystoisospora spp. and Microsporidia may cause severe diarrhoea among immunocompromised patients. There scanty data on their frequency immunocompetent Accordingly, we studied the of enteric opportunistic parasites patients with from northern India; also performed genetic characterisation Cryptosporidia them. Patients Methods: Stool samples 80...

Abstract Background and Aim Lactase non‐persistence is common in I ndia. We evaluated: (i) frequency of lactase gene ( C / T ‐13910 G A ‐22018) polymorphisms irritable bowel syndrome IBS ) healthy controls HC ), (ii) association between these ‐subtypes symptoms. Methods total 150 patients R ome‐ III criteria) 252 age gender‐matched were evaluated for ‐22018 genotypes using polymerase chain reaction‐restriction fragment length polymorphism PCR‐RFLP ). Results Totals 79 (52%), 52 (35%) 19...

Cryptosporidiosis is intestinal opportunistic infection commonly occurring in immunocompromised patients including renal transplant (RT) recipients receiving continuous immunosuppressive therapy. Knowledge about species of Cryptosporidium-infecting RT necessary to know mode its transmission (anthroponotic or zoonotic). Various genes such as small subunit rRNA (SSU rRNA) and Cryptosporidium oocyst wall protein (COWP) may help identification though their sensitivity specificity are highly...

Genetically diverse parasites enhances resistance against antimalarials, vaccines and host immune responses. The present study was designed to evaluate the role played by Plasmodium falciparum genetic diversity in predicting real world malarial population. Initially, incidence pattern of all four northern Indian species examined using 18S rRNA gene performed principal component analysis (PCA) based on frequencies species. Consequently, variance histidine-rich protein-2 (Pfhrp2) among...

Hepatitis C virus (HCV) infection is a considerable public‐health problem and an important cause of liver disease with about 71 million people infected worldwide more than 399 000 die every year from hepatitis C‐related diseases. The present study was, therefore, initiated to investigate the association polymorphism in interferon λ3 (IFNL3) also known as interleukin‐28B (IL‐28B) gene chronic HCV these polymorphic variants combination daclatasvir sofosbuvir therapy response. Genotypes were...

Polymerase chain reaction (PCR) is a technique involving enzymatic amplification of nucleic acid sequences in repeated cycles denaturation, oligonucleotide annealing and DNA polymerase extension. It powerful molecular biologic tool that allows the rapid production analytic quantities from small amounts starting material. PCR can be performed on nearly any ocular specimen or biopsy. For diagnosis uveitis, obtained sample usually an anterior chamber paracentesis vitreous tap. potentially more...

Complex chromosomal rearrangements (CCRs) are rare structural involving three or more chromosomes. Balanced CCRs often seen in phenotypically normal females who may later present with fertility problems. We report a 25-year old female an adverse obstetric history and de novo CCR chromosomes 7, 13 15 detected after GTG-banding. The origin was confirmed because the karyotypes of proband’s parents male sibling were normal. Cytogenetic analysis proband revealed following complex karyotype:...

Objectives: Deposition of body fat may differ in different age groups and gender-wise differences are also likely. The present study aims to evaluate age- obesity parameters healthy subjects both genders Varanasi. Materials Methods: Anthropometric measurements were performed 346 reported health check-up camp. Parameters compared between each group for males females separately as well comparison was performed. Regression analysis observe the relationship with mass index (BMI) other obesity....