A5042925964- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- CRISPR and Genetic Engineering
- Genetic Mapping and Diversity in Plants and Animals
- Gene expression and cancer classification
- Genetic and phenotypic traits in livestock
- Pregnancy and preeclampsia studies
- Renal and related cancers
- Cancer-related Molecular Pathways
- RNA regulation and disease
- interferon and immune responses
- Genetic diversity and population structure
- Microtubule and mitosis dynamics
- Reproductive System and Pregnancy
- RNA Research and Splicing
Western University
2021-2025
Remodeling of the uterine vasculature by invasive extravillous trophoblasts (EVTs) is a critical aspect human placentation. Insufficient EVT invasion can lead to severe obstetrical complications like preeclampsia, intrauterine growth restriction, and preterm birth. Glial cells missing-1 (GCM1) transcription factor that crucial for proper placentation in mice, highly expressed syncytiotrophoblast (ST) EVTs. GCM1 classically considered master regulator ST formation, but little known about its...
Summary Ku70 and Ku80 form Ku, a ring-shaped protein that initiates the non-homologous end-joining (NHEJ) DNA repair pathway. 1 Specifically, Ku binds to double-stranded (dsDNA) ends recruits other NHEJ factors ( e.g. , DNA-PKcs LIG4). While RNA (dsRNA) 2 traps mutated-DNA-PKcs on ribosomal in vivo, 3,4 physiological significance of Ku-dsRNA interactions otherwise wild-type cells remains elusive. Intriguingly, while dispensable for murine development, 5,6 is essential human cells. 7 Despite...
The Ku70/80 heterodimer is a key player in non-homologous end-joining DNA repair but involved other cellular functions like telomere regulation and maintenance, which Ku's role not fully characterized. It was previously reported that knockout of Ku80 human cell line results lethality, the underlying cause Ku essentiality cells has yet to be explored. Here, we established conditional Ku70 using CRISPR/Cas9 editing study function. While observed loss viability upon depletion, did detect...
Abstract In the study of genetic diversity in non-model species there is a notable lack low-cost, high resolution tools that are readily available for model organisms. Genotyping microarray technology organisms well-developed, affordable, and potentially adaptable cross-species hybridization. The Mouse Diversity Array (MDGA), single nucleotide polymorphism (SNP) genotyping tool designed Mus musculus , was tested as to survey genomic wild inter-order, inter-genus, intra-genus comparisons....
Abstract The Ku70/80 heterodimer is a key player in non-homologous end-joining DNA repair but has also been involved other cellular functions like telomere regulation and maintenance, which Ku’s role not fully characterized. It was previously reported that knockout of Ku80 human cell line results lethality, the underlying cause Ku essentiality cells yet to be explored. Here, we established conditional Ku70 study function. Endogenous achieved using CRISPR/Cas9 editing where expression...