A5051903509- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Acute Lymphoblastic Leukemia research
- Genomics and Chromatin Dynamics
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
Max Delbrück Center
2013-2016
Dendritic cells (DCs) are essential regulators of immune responses; however, transcriptional mechanisms that establish DC lineage commitment poorly defined. Here, we report the PU.1 transcription factor induces specific remodeling higher-order chromatin structure at interferon regulatory 8 (Irf8) gene to initiate fate choice. An Irf8 reporter mouse enabled us pinpoint an initial progenitor stage which DCs separate from other myeloid lineages in bone marrow. In absence Irf8, this undergoes...
There are groups of genes that need coordinated repression in multiple contexts, for example if they code proteins work together a pathway or protein complex. Redundancy biological regulatory networks implies such might occur at both the pre- and post-transcriptional level, though not necessarily simultaneously under same conditions. Here, we propose redundancy global network can be detected by overlap between putative targets transcriptional repressor, as identified ChIP-seq experiment,...
Over-representation of predicted miRNA targets in sets genes regulated by a given transcription factor (e.g. as defined ChIP-sequencing experiments) helps to identify biologically relevant and is useful get insight into post-transcriptional regulation.To facilitate the application this approach we have created mBISON web-application. calculates significance over-representation non-ranked gene set. The set can be specified either list or one more ChIP-seq datasets followed user-defined...