A5070858411- Algal biology and biofuel production
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Protein Kinase Regulation and GTPase Signaling
- Photosynthetic Processes and Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Vibrio bacteria research studies
- Enzyme Structure and Function
- Cystic Fibrosis Research Advances
- Adipose Tissue and Metabolism
- Lipid Membrane Structure and Behavior
- Biochemical and Molecular Research
- Bacterial biofilms and quorum sensing
- Pluripotent Stem Cells Research
- Glycosylation and Glycoproteins Research
- Photoreceptor and optogenetics research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Marine and coastal ecosystems
- 3D Printing in Biomedical Research
- Melanoma and MAPK Pathways
- Salmonella and Campylobacter epidemiology
- Cancer-related gene regulation
- Bacteriophages and microbial interactions
- Neuroscience and Neuropharmacology Research
Western University
2008-2023
Springer Nature (Germany)
2014
The derivation and long-term maintenance of human embryonic stem cells (hESCs) has been established in culture formats that are both dependent independent support (feeder) cells. However, the factors responsible for preserving viability hESCs a nascent state remain unknown. We describe mass spectrometry-based method probing secretome hESC microenvironment to identify potential regulating protein low abundance. Individual samples were analyzed several times, using successive (m/z) retention...
Burkholderia cenocepacia is an opportunistic pathogen threatening patients with cystic fibrosis. Flagella are required for biofilm formation, as well adhesion to and invasion of epithelial cells. Recognition flagellin via the Toll-like receptor 5 (TLR5) contributes exacerbate B. cenocepacia-induced lung inflammatory responses. In this study, we report that glycosylated on at least 10 different sites a single sugar, 4,6-dideoxy-4-(3-hydroxybutanoylamino)-d-glucose. We have identified key...
Chlamydomonas sp. UWO 241 (UWO 241) is a psychrophilic green alga isolated from Antarctica. A unique characteristic of this algal strain its inability to undergo state transitions coupled with the absence photosystem II (PSII) light-harvesting complex protein phosphorylation. We show that preferentially phosphorylates specific polypeptides associated an approximately 1,000-kD pigment-protein supercomplex contains components both I (PSI) and cytochrome b 6/f (Cyt 6/f) complex. Liquid...
Campylobacter jejuni is the leading cause of bacterial gastroenteritis. It relies on several virulence factors for host colonization, including glycosylated flagella. C. NCTC 11168 modifies its flagellins with pseudaminic acid derivatives. also presumed to modify these proteins legionaminic acid, although no glycopeptide evidence was available at onset this study. The enzyme encoded by cj1319 can be used make in vitro, but pathway synthesis partially inferred knockout mutagenesis coli VC167...
Specificity is a limiting factor when using small-molecule inhibitors to study protein kinase signalling. Since inhibitor-resistant mutants (i.e., drug-resistant alleles) remain active in the presence of inhibitor, they facilitate validation on-target effects. By combining an mutant with mass spectrometry-based phosphoproteomics, we previously devised systematic strategy for reliable identification and CSNK2 substrates. In this study, use same evaluate selectivity CX-4945, clinical stage...
Summary The objective of this work was to characterize photosynthetic ferredoxin from the Antarctic green alga Chlamydomonas sp. UWO 241, a key enzyme involved in distributing reducing power. We hypothesize that possesses characteristics typical cold‐adapted enzymes, namely increased structural flexibility and high activity at low temperatures, accompanied by stability moderate temperatures. To address objective, we purified 241 characterized temperature dependence its enzymatic protein...
Casein Kinase 2 (CSNK2) is an extremely pleiotropic, ubiquitously expressed protein kinase involved in the regulation of numerous key biological processes. Mapping CSNK2-dependent phosphoproteome necessary for better characterization its fundamental role cellular signalling. While ATP-competitive inhibitors have enabled identification many putative substrates, compounds targeting highly conserved ATP-binding pocket often exhibit off-target effects limiting their utility definitive...
Neurons within different brain regions have varying levels of vulnerability to external stress and respond differently injury. A potential reason explain this may lie a key lipid class the cell's plasma membrane called gangliosides. These glycosphingolipid species been shown play various roles in maintenance neuronal viability. The purpose study is use electrospray ionization mass spectrometry (ESI-MS) immunohistochemistry evaluate temporal expression profiles gangliosides during course...
Mass spectrometry has become a widely used analytical technique for proteomics study because of its high throughput and sensitivity. Among those applications, specific one is to characterize glycan structure. Glycosylation frequently occurred post-translational modification proteins which relevant humans' health. Therefore, it significant develop effective computational methods automate the identification structures from mass spectral data. In our research, we mathematically formulated de...