Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated9 (Cas9) endonuclease system is a powerful RNA-guided genome editing tool. CRISPR/Cas9 has been well studied in model plant species for targeted editing. However, few studies have reported on without whole sequence information. Currently, no study performed to manipulate metabolic pathways using CRISPR/Cas9. In this study, the type II CRISPR/SpCas9 was used knock out, via nonhomologous end-joining...

Plants are frequently exposed to microorganisms like fungi, bacteria, and viruses that cause biotic stresses. Fusarium head blight (FHB) is an economically risky wheat disease, which occurs upon graminearum (Fg) infection. Moderately susceptible (cv. "Mizrak 98") "Gun 91") winter type bread cultivars were subjected transcriptional profiling after exposure Fg To examine the early response pathogen in wheat, we measured gene expression alterations mock inoculated root crown of moderately (MS)...

Alkaloids are diverse group of secondary metabolites generally found in plants. Opium poppy (Papaver somniferum L.), the only commercial source morphinan alkaloids, has been used as a medicinal plant since ancient times. It produces benzylisoquinoline alkaloids (BIA) including narcotic analgesic morphine, muscle relaxant papaverine, and anti-cancer agent noscapine. Though BIAs play crucial roles many biological mechanisms their steps biosynthesis responsible genes remain to be revealed. In...

Papaver bracteatum Lindl., P. orientale L., and pseudo-orientale (Fedde) Medw., species of section Oxytona Bernh. in the genus are found widely native flora Turkey. They commercially important for their high alkaloid content. Because has similar morphological characters, it is not easy to distinguish these species. We used inter-simple sequence repeat (ISSR) molecular marker system determine characterisation this section. In study, 20 ISSR primers have been studied 180 accessions collected...

Giriş: Bu çalışma, Sağlık Hizmetleri Meslek Yüksekokulu öğrencilerinin Geleneksel ve Tamamlayıcı Tıp (GETAT) uygulamaları hakkındaki bilgi düzeylerini tutumlarını belirlemek davranışlarını değerlendirmek amacıyla yapılmıştır. Materyal Metot: Tanımlayıcı tipte olan bu çalışma Çankırı Karatekin Üniversitesi Eldivan Yüksekokulu’nda öğrenim gören 1. 2. sınıf öğrencileri arasında 2019 yılı Ocak-Mart ayları gerekli izinler alınarak Araştırmanın örneklemini çalışmaya katılmayı kabul eden toplam 264...

The benzylisoquinoline alkaloids (BIA) are secondary metabolites that produced by many of the Papaver species including bracteatum, pseudo-orientale, and orientale, three representative members section Oxytona Bernh. sequences expression levels genes positioned on BIA biosynthesis pathway were previously defined in opium poppy (Papaver somniferum), one mostly studied same genus. Nevertheless, majority predicted BIA-related gene have not been specified. Here we presenting new cDNA belong to...

In this study we aimed to evaluate the morphological traits and essential oil compositions of three members Astreraceae ( Helichrysum plicatum subsp. DC., Tanacetum vulgare L., Anthemis cretica L. albida (Boiss.) Grierson) growing wild in semi-arid areas Cankiri province, Turkey. Some characters were reported for first time. Essential oils obtained by hydrodistillation, their components identified using gas chromatography–mass spectrometry (GC-MS). Forty eight compounds detected 39 15...

Endüstriyel ve tıbbi açıdan önemli bir bitki olan Papaver somniferum L. (haşhaş) uzun kullanım geçmişine sahiptir. Haşhaş, morfin, kodein, tebain, papaverin, noskapin narsein gibi güçlü alkaloidleri üretmektedir. Bu alkaloidler, ağrı kesici (analjezik), öksürük bastırıcı (antitusif) bazı durumlarda yatıştırıcı (sedatif) etkilere Morfin, kodein diğer şiddetli ağrıları hafifletmek için kullanılan analjeziklerdir. nedenle, cerrahi operasyonlar veya durumlarında kullanılmaktadırlar. Uygun...

<title>Abstract</title> Long noncoding RNAs (lncRNAs) are essential for transcription and involved in a wide range of biological processes plants. Although lncRNAs have been identified various plants, research on those linked to alkaloid biosynthesis <italic>Papaver somniferum</italic> L. is limited. In this study, the expression two opium poppy cultivars that produce different major alkaloids (morphine noscapine) were analysed. A total 8945 lncRNAs, mainly lincRNAs, characterized cultivars'...

Circadian rhythm is defined biochemical, physiological, metabolic and behavioral responses of all living organisms such as bacteria, mammals, fungi plants within a 24-hour light dark photoperiod. In plants, circadian regulates multiple processes stomatal leaf movements, stem elongation, enzyme activity aging. clock genes are known to play role in regulating the biosynthesis phytohormones which involved many crucial seed germination, hypocotyl elongation photosynthesis. optimizes...

Cucumber (Cucumis sativus L.), one of the 30 Cucumis species found worldwide, is most economically significant. Specified that Pseudoperonospora cubensis causes downy mildew, destructive foliar diseases affecting cucumbers and other cucurbits. Using a combination QuEChERS technique HPLC, residue levels rate degradation commercial fungicide, contained two active components (metalaxyl-M chlorothalonil), in cucumber were determined. Additionally, fungicide's effectiveness was assessed. The...

Papaver somniferum is a medicinal plant of the Papaveraceae family that has traditionally been used for diet or its therapeutic value thousands years. Mainly, morphine and noscapine alkaloids exhibit anti-analgesic anti-cancer effects. However, gene expression patterns regulatory elements, such as transcription factors between different tissues, still need to be detected. In this study, comparative in silico transcriptome analyses were conducted examine tissue-specificity benzylisoquinoline...

Schistosomiasis caused by Schistosoma parasites is one of the most common parasitic infections worldwide. Genetic regulation genus Schistosoma, which has different developmental stages throughout its life, quite complex. In these parasites, thousands long noncoding RNAs (lncRNAs) estimated to be functional were identified. Identifying transcripts expressed in and detecting their functions for better understanding role lncRNAs require a comparative study.Assembled RNA-seq datasets belonging...

Transposable elements (TEs) are the most abundant group of genomic in plants that can be found genic or intergenic regions their host genomes. Several stimuli such as biotic abiotic stress have roles either activating transcription transposition. Here effect Panicum mosaic virus (PMV) and its satellite (SPMV) infection on transposon Brachypodium distachyon model plant was investigated. To evaluate activity TEs, transcriptomic data mock inoculated were compared. Our results indicate major...

<strong>Objective</strong>: Strongyloides stercoralis, which is a soil-borne helminth, affects tens of millions people in the whole world, and may cause life-threatening chronic infections. In this study, purpose was to examine data reported strongyloidiasis cases Turkey on age, gender, clinical findings, underlying diseases, diagnostic methods treatment modalities by using pooling analysis method. <br> <strong>Material Method</strong>: stercoralis were accessed reviewing two national...

Objective: The objective of this study is to review the studies on leishmaniasis since 40 years and evaluate increasing contribution Turkey in terms globally.&#x0D; Material Method: literature data included all research publications Web Science database between January 1980 December 2019. Language or document type limitations were not used. To publication effect Turkey, analysis function database, “Turkey” was chosen as country topic recorded based their subject areas yearly counts.&#x0D;...