A5074193948- Immune Response and Inflammation
- Systemic Lupus Erythematosus Research
- Herpesvirus Infections and Treatments
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- T-cell and Retrovirus Studies
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- RNA Research and Splicing
- Inflammasome and immune disorders
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Natural product bioactivities and synthesis
- Hepatitis C virus research
- Animal Virus Infections Studies
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- Plant biochemistry and biosynthesis
- Toxin Mechanisms and Immunotoxins
- Plant chemical constituents analysis
- Parvovirus B19 Infection Studies
National Research Centre
2018-2024
National Centre for Research and Development
2024
Ministry of Health of the Russian Federation
2016-2024
Gamalei Institute of Epidemiology and Microbiology
1997-2022
Russian Academy of Sciences
2002-2010
Academy of Medical Sciences
2001-2010
Czech Academy of Sciences, Institute of Experimental Medicine
2008
University of Chieti-Pescara
1995
We have analysed the structure of mRNA isoforms human gene encoding tryptophanyl‐tRNA synthetase (Trp‐tRNA synthetase) expressed in epithelial CaOv cells and MT‐4 lymphocytes. The Trp‐tRNA is induced by interferon‐γ both lines and, lymphocytes, also interferon‐α. Four different combinations first exons IA, IB II. Two transcription initiation sites (P1 P2) were detected 90 bp from each other. Processing primary transcript initiated PI start site generates isoform where exon IA joins to other...
Successful formation of electronic interfaces between living cells and components requires both good cell viability performance level. This paper presents a technology for the nanostructured arrays multi-walled carbon nanotubes (MWCNT) in biopolymer (albumin) layer higher biocompatibility. The liquid albumin dispersion was sprayed on synthesized MWCNT by deposition system. These nanostructures were engineered using nanosecond pulsed laser radiation mapping near-IR spectral range (λ = 1064...
After incubation of human monocytes J96 and myeloid leukemia HL60 cells with interferons (IFN) α or γ, the Ap 3 A concentration considerably increases in parallel accumulation tryptophanyl‐tRNA synthetase (TrpRS, EC 6.1.1.2) The formation response to IFNs is catalysed by an excessive amount TrpRS. Although function still remain unknown its may imply involment IFN‐signalling pathway.
The review contains a brief analysis of the 60-year history discovery, study and medical application interferons, new group remarkable proteins that have found wide in therapy virological, oncological, neurological, ophthalmic other pathologies. Modern data on classification interferons mechanisms their action are given. Particular attention is paid to clinical use medications interferon its inducers.Обзор содержит краткий анализ 60-летней истории открытия, изучения и медицинского применения...
Hepatitis C virus (HCV) is one of the major causes chronic liver disease and leads to cirrhosis hepatocarcinoma. Despite extensive research, there still no vaccine against HCV. In order induce an immune response in DBA/2J mice HCV, we obtained modified mouse mesenchymal stem cells (mMSCs) simultaneously expressing five nonstructural HCV proteins (NS3-NS5B). The innate mMSCs was higher than DNA immunization, with plasmid encoding same proteins, naïve unmodified MSCs. triggered strong...
The coronavirus disease (COVID-19) pandemic has brought into sharp relief the threat posed by coronaviruses and laid foundation for a fundamental analysis of this viral family, as well search effective anti-COVID drugs. Work is underway to update existent vaccines against COVID-19, screening low-molecular-weight drug candidates outpatient medicine continues. opportunities ways accelerate development antiviral drugs other pathogens are being discussed in context preparing next pandemic. In...
Наровлянский А.Н
Abstract Nitric oxide is a diffusible messenger with multiple biological functions. We show here that NO‐generating compound, S‐nitrosoglutathione (GSNO) induces apoptosis in human chondrocytes and causes necrosis‐like celt death epithelial CaOv cell line. Pretreatment of low‐dose GSNO or γ‐interferon enhances their tolerance to the second high‐concentration exposure. On contrary, cells pretreatment diminishes resistance increases cytolysis at conclude possess specific inducible mechanism...
Activation of Toll-like receptors (TLRs) is one the earliest indicators functional activation innate immune system. Therefore, development drugs that stimulate transcription TLR/RLR genes and at same time are “multi-target” an important task modern immunopharmacology. In this regard, antiviral combine properties interferonogens immunomodulators, which also include Cycloferon® its analogues, great interest. The purpose study was to assess expression determine signalling reactions system under...
The aim of the research was to study population composition splenic lymphoid cells, assess functional activity lymphocytes as well state gastrointestinal tract microbiota in experimental modeling metabolic syndrome (MS). studies were conducted using two models MS and hyperlipidemia (HL), based on prolonged drinking animals with 20% aqueous fructose solution added cholesterol intraperitoneal administration Poloxamer 407 mice, respectively. results experiments indicate a change splenocytes...
Objective. The work was performed with the purpose to study microbiota state of gastro-intestinal tract (GIT) C57Bl/6 mice signs metabolic dysbiosis (MD) induced use fructose solution or poloxamer 407 (P 407), during treatment combination sodium polyprenylphosphate (PPP) and beta-sitosterol (BSS). Materials Methods. MD in drinking 20 per cent water solution. also by intraperitoneal P at dose 500 mg/kg two times week ten weeks. experimental groups received intragastrically PPP BSS...
Ongoing outbreaks and often rapid spread of infections caused by coronaviruses, influenza, Nipah, Dengue, Marburg, monkeypox, other viruses are a concern for health authorities in most countries. Therefore, the search study new antiviral compounds great demand today. Since almost all with pandemic potential have immunotoxic properties various origins, particular attention is paid to development immunomodulatory drugs. We synthesised compound related indole-3-carboxylic acid derivatives...
Introduction. Cytokines activated in response to immunosuppressive viral infections can directly or indirectly affect the neoplastic transformation of B cells. In this study, we studied a new substance designed produce antiviral drug CelAgrip (CA, CelAgripus), which exhibits interferon (IFN) and cytokine-inducing activity and, apparently, be used as an activator immunity.Purpose - is evaluate cytokine-regulating effect CA Burkitt's lymphoma (LB) cell lines latently infected with Epstein-Barr...
For more than 60 years, the study of interferons (IFN) system has continued, as a complex network system. IFNs 3 types are discovered: I (α/β), II (ƴ), III (λ), their interrelations, mechanisms action, functional diversity investigated. The practical way out capacity human peripheral blood leukocytes to produce IFN was method determining interferon status, which allows judge immunoreactivity organism, detect sensitivity cells immunoac-tive drugs and makes it possible determine tactics...
Synthesis of mRNAs for the key immunoactive cytokines was studied in mononuclear cells (MNC) from human peripheral blood, using reverse transcription and polymerase chain reaction, after incubation isolated with serum γ-globulin, zinc ions, or zinc-γ-globulin metal complexes. A constitutive presence interferon-α (IFNα) interleukin-18 (IL-18) mRNA shown MNC all subjects observed, whereas IFNγ, IL-4, IL-8 synthesis not detectable. Expression IL-1β, IL-2, IL-6 и IL-10 revealed only a half...