A5086059652- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Caveolin-1 and cellular processes
- Chromosomal and Genetic Variations
- Genetics and Neurodevelopmental Disorders
- Sphingolipid Metabolism and Signaling
- Erythrocyte Function and Pathophysiology
University of California, San Francisco
2016-2019
Abstract CRISPR/Cas technologies have transformed our ability to add functionality the genome by knock-in of payload via homology-directed repair (HDR). However, a systematic and quantitative profiling integration landscape is still lacking. Here, we present framework based on long-read sequencing an integrated computational pipeline (knock-knock) analyze outcomes across wide range experimental parameters. Our data uncover complex profiles, with perfect HDR often accounting for minority...
TET enzymes convert 5-methylcytosine to 5-hydroxymethylcytosine and higher oxidized derivatives. TETs stably associate with are post-translationally modified by the nutrient-sensing enzyme OGT, suggesting a connection between metabolism epigenome. Here, we show for first time that modification OGT enhances TET1 activity in vitro. We identify domain is necessary sufficient binding report point mutation disrupts TET1-OGT interaction. this interaction rescue hematopoetic stem cell production...
Significance Naive and primed pluripotent stem cells (PSCs) provide a potential source of for regenerative medicine. Although both cell types can contribute to all three germ layers, they differ in morphology, gene expression programs, epigenetic modifications, such as the X chromosome inactivation status. Here, we report that lysophosphatidic acid (LPA) lipid signaling LPA-producing enzyme autotaxin are crucial converting PSCs into naive PSCs. Our results reveal relationships between...