A5058739673- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Viral-associated cancers and disorders
- Bacteriophages and microbial interactions
- Polyomavirus and related diseases
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Parvovirus B19 Infection Studies
- Genetic factors in colorectal cancer
- Genomics and Chromatin Dynamics
- Ferroptosis and cancer prognosis
- Colorectal Cancer Treatments and Studies
- Lymphoma Diagnosis and Treatment
- RNA Research and Splicing
- Genetics, Bioinformatics, and Biomedical Research
- RNA modifications and cancer
- Histiocytic Disorders and Treatments
- Digestive system and related health
- Evolution and Genetic Dynamics
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Environmental DNA in Biodiversity Studies
- Biosensors and Analytical Detection
Ministry of Health of the Russian Federation
2019-2024
Russian Cancer Research Center NN Blokhin
2015-2019
Cancer Research Center
2018
Russian Academy of Sciences
2005-2014
Academy of Medical Sciences
2009-2014
The Epstein–Barr virus (EBV) plays a key role in the development of undifferentiated nasopharyngeal carcinoma (uNPC). In uNPC endemic regions EBV-specific antibodies and plasma EBV DNA load are used as markers for early detection monitoring disease. non-endemic regions, such studies were practically not conducted. aim this study was to compare clinical significance serological levels patients region, Russia. results obtained indicate that both viral capsid antigen/immunoglobulin A (VCA/IgA)...
Isotachophoresis is an electrophoretic method of separation charged substances. The characterized by a discontinuous buffer system, constant velocity separated molecules, and the distribution components in form narrow concentrated bands located one right after another. As rule, isotachophoresis not used for nucleic acids because mobility polynucleotides this system does depend on their size. However, circumstance proved to be very useful quantitative isolation heterogeneous DNA fragments...
The data in this article are related to the research entitled "Optimization of melting analysis with TaqMan probes for detection KRAS, NRAS, and BRAF mutations" Botezatu et al. [1]. Somatic mutations PIK3CA gene ("hot spots" exons 9 20) found many human cancers, their presence can determine prognosis a treatment strategy. An effective method mutation scanning clinical laboratories is DNA Melting Analysis (DMA) (Vorkas al., 2010; Simi 2008) [2], [3]. It was demonstrated recently that which...
Asymmetric PCR and DNA melting analysis with TaqMan probes applied for mutation detection is effectively used in clinical diagnostics. The method simple, cost-effective, carried out a closed-tube format, minimizing time, labor, risk of sample cross-contamination. Although more sensitive than Sanger sequencing (mutation thresholds are ∼5% 15%–20%, respectively), it less labor-intensive expensive techniques such as pyrosequencing droplet digital PCR. Here, we demonstrate that, under specially...
Abstract DNA Melting Analysis (DMA) with a TaqMan probe covering the mutation “hot spot” is simple, sensitive, and “closed tube” method of detection. However, DMA requires asymmetric PCR to produce single-stranded amplicons capable interacting probes. This makes quantitative analysis impossible owing low amplification efficiency. Moreover, bi-strand detection necessitates two independent PCRs. The SLAM-MS ( S tem- L oop AM plicon M utation canning) assay, in which symmetric performed using...
Epstein–Barr virus (EBV), a representative of the herpesvirus family, is etiological agent for number benign and malignant human neoplasms. Among latter, nasopharyngeal carcinoma (NPC) occupies special place. In NPC development EBV plays key role stimulating progression pathological process from precancerous lesions to cancer development. For most patients, elevated levels humoral IgG IgA antibodies against capsid early antigens are characteristic their antibody titers rise high long before...
Introduction. Local hypermethylation of gene promoters and global genome hypomethylation are well-known manifestations aberrant methylation associated with carcinogenesis. We investigated this phenomenon as a possible diagnostic marker for liquid biopsy colorectal cancer using the original quantitative DNA melting analysis hybridiza-tion probes (qDMA-HP) method. Aim. To quantify HIST1H4F promoter LINE-1 transposon in circulating blood plasma patients. Materials methods. Bisulfite-treated...
Scanning for mutations by DNA melting analysis (DMA) is based on asymmetric PCR followed the of duplexes formed single-stranded amplicons with TaqMan probes. The method optimally suited clinical genetic testing; it easy to perform, high-throughput, and sensitive. detection limit mutant alleles DMA about 3%, which much higher than sensitivity Sanger sequencing. In addition, realized in a closed-tube format, while 2-h assay carried out single tube without any intermediate or additional...
DNA Melting Analysis is very effective in clinical diagnostics: it simple to perform, high throughput, labor-, time- and cost-effective implemented the “closed tube” format minimizing risk of samples cross-contamination. Although more sensitive than sequencing by Sanger (mutant allele detection limit ~5 ~15 % respectively), it, however, inferior this respect some other, laborious expensive methods (in particular, ddPCR (digital droplet PCR)). Using BRAF gene as a prototype, we developed...