A5039852728- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Identification and Quantification in Food
- Yersinia bacterium, plague, ectoparasites research
- Zoonotic diseases and public health
- SARS-CoV-2 and COVID-19 Research
- Bacterial Genetics and Biotechnology
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- Fire effects on ecosystems
- Vibrio bacteria research studies
- vaccines and immunoinformatics approaches
- Environmental DNA in Biodiversity Studies
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Genomics and Phylogenetic Studies
Robert Koch Institute
2014-2023
Institut für Mikrobiologie der Bundeswehr
2020-2023
Universität der Bundeswehr München
2020-2023
Czech Academy of Sciences, Institute of Microbiology
2021
Abstract The severe Ebola virus disease epidemic occurring in West Africa stems from a single zoonotic transmission event to 2‐year‐old boy Meliandou, Guinea. We investigated the origins of using wildlife surveys, interviews, and molecular analyses bat environmental samples. found no evidence for concurrent outbreak larger wildlife. Exposure fruit bats is common region, but index case may have been infected by playing hollow tree housing colony insectivorous free‐tailed ( Mops condylurus )....
Through full genome analyses of four atypical Bacillus cereus isolates, designated B. biovar anthracis, we describe a distinct clade within the group that presents with anthrax-like disease, carrying virulence plasmids similar to those classic anthracis. We have isolated members this from different mammals (wild chimpanzees, gorillas, an elephant and goats) in West Central Africa (Côte d'Ivoire, Cameroon, African Republic Democratic Congo). The isolates shared several phenotypic features...
Bacillus cereus biovar anthracis (Bcbva) is a member of the B. group which carries both virulence plasmids, causes anthrax-like disease in various wildlife species and was described several sub-Saharan African rainforests. Long-term monitoring carcasses Taï National Park, Côte d'Ivoire, revealed continuous mortality due to Bcbva broad range mammalian species. While non-lethal anthrax infections have been for anthracis, nothing known about odds survival following an infection caused by Bcbva....
We are currently facing a pandemic of COVID-19, caused by spillover from an animal-originating coronavirus to humans occurring in the Wuhan region China December 2019. From China, virus has spread 188 countries and regions worldwide, reaching Sahel on 2 March 2020. Since whole genome sequencing (WGS) data is very crucial understand spreading dynamics ongoing pandemic, but only limited available date, we have focused our efforts generating first Malian available. Screening 217 patient samples...
Analysis of 16S rRNA (rRNA) genes provides a central means taxonomic classification bacterial species. Based on presumed sequence identity among species the Bacillus cereus sensu lato group, B. anthracis have been considered unsuitable for diagnosis anthrax pathogen. With recent identification single nucleotide polymorphism in some gene copies, specific becomes feasible. Here, we designed and evaluated set situ, vitro, silico assays to assess unknown state from different perspectives. Using...
Abstract The metabarcoding of vertebrate DNA found in invertebrate‐derived (iDNA) has proven a powerful tool for monitoring biodiversity. To date, iDNA primarily been used to detect the presence/absence particular taxa using metabarcoding, though recent efforts demonstrated potential utility these data estimating relative animal abundance. Here, we test whether can also be reconstruct complete mammalian mitogenomes and therefore bring field closer population‐level analyses. Specifically,...
Bacillus cereus biovar anthracis (Bcbva) is an untypical pathogen causing a fatal anthrax-like disease in variety of wildlife species African rainforest areas. In contrast to and most the B. group, all strains Bcbva cluster contain 22 kb insertion sigK gene which encodes essential late sporulation sigma factor σK. This excised during site-specific recombination process resulting intact circular molecule. The kinetics two each were compared by expression analysis eight sporulation-associated...
Abstract Analysis of 16S ribosomal RNA (rRNA) genes provides a central means taxonomic classification bacterial species. Based on presumed sequence identity among species the Bacillus cereus sensu lato group, rRNA B. anthracis have been considered unsuitable for diagnosis anthrax pathogen. With recent identification single nucleotide polymorphism in some gene copies, specific becomes feasible. Here, we designed and evaluated set situ-, vitro- silico- assays to assess yet unknown 16S-state...
Abstract A number of RT-qPCR assays for the detection SARS-CoV-2 have been published and are listed by WHO as recommended assays. Furthermore, numerous commercial with undisclosed primer probe sequences on market. As pandemic progresses, virus accrues mutations, which in some cases – seen B.1.1.7 variant can outperform push back other strains SARS-CoV-2. If mutations occur or binding sites, this impact results impede diagnostics. Here we tested effect mismatches performance vitro using...