A5081952035- Monetary Policy and Economic Impact
- Economic theories and models
- Economic Theory and Policy
- Fiscal Policy and Economic Growth
- Fiscal Policies and Political Economy
- Gene Regulatory Network Analysis
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Bioinformatics and Genomic Networks
- Fungal and yeast genetics research
- Global Financial Crisis and Policies
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Advanced Photocatalysis Techniques
- vaccines and immunoinformatics approaches
- Expert finding and Q&A systems
- Clostridium difficile and Clostridium perfringens research
- Income, Poverty, and Inequality
- Evolutionary Algorithms and Applications
- Machine Learning in Materials Science
- Biofuel production and bioconversion
- Gut microbiota and health
- Economic Growth and Productivity
Broad Institute
2014-2024
Massachusetts Institute of Technology
2014-2024
Moscow Institute of Thermal Technology
2022
MIT University
2022
University of California, Los Angeles
2021
Harvard University Press
2019
Clemson University
1983-2019
Agilent Technologies (United States)
2006-2013
Whitehead Institute for Biomedical Research
2002-2006
Walker (United States)
1983-2005
We have determined how most of the transcriptional regulators encoded in eukaryote Saccharomyces cerevisiae associate with genes across genome living cells. Just as maps metabolic networks describe potential pathways that may be used by a cell to accomplish processes, this network regulator-gene interactions describes yeast cells can use regulate global gene expression programs. information identify motifs, simplest units architecture, and demonstrate an automated process motifs assemble...
A discretionary policymaker can create surprise inflation, which may reduce employment and raise government revenue. But when people understand the policymaker's objectives, these surprises cannot occur systematically. In equilibrium form expectations rationally optimizes in each period, subject to way that expectations. Then, we find (1) rates of monetary growth inflation are excessive; (2) depend on slope Phillips curve, natural unemployment rate, other variables affect benefits costs from...
The transcriptional regulatory networks that specify and maintain human tissue diversity are largely uncharted. To gain insight into this circuitry, we used chromatin immunoprecipitation combined with promoter microarrays to identify systematically the genes occupied by regulators HNF1alpha, HNF4alpha, HNF6, together RNA polymerase II, in liver pancreatic islets. We identified tissue-specific circuits formed HNF6 other transcription factors, revealing how these factors function as master of...
Abstract Background The regulatory map of a genome consists the binding sites for proteins that determine transcription nearby genes. An initial S. cerevisiae was recently published using six motif discovery programs to analyze genome-wide chromatin immunoprecipitation data 203 factors. were used identify sequence motifs likely correspond DNA-binding specificity immunoprecipitated proteins. We report improved versions two conservation-based algorithms, PhyloCon and Converge. Using these...
It is currently popular to identify monetary policy shocks with innovations in some measure of reserves or the federal funds rate. These assumptions about interest elasticity supply demand for imply that produce dynamic responses macroeconomic variables are anomalous relative traditional analyses. This paper tentatively identifies and markets M2 1980s contrasts them results 1970s. In later period, identified have impacts fully consistent
Rules, Discretion and Reputation in a Model of Monetary Policy Abs tractIn discretionary regime the monetary authority can print more money create inflation than people expect.But, although these surprises have some benefits, they cannot arise systematically equilibrium when understand policymakor's incentives form their expectations accordingly.Because policymaker has power to shocks ex post, growth rates prices turn out be higher otherwise.Therefore, enforced commitments (rules) for...
Centralized facilities for genetic engineering, or "biofoundries", offer the potential to design organisms address emerging needs in medicine, agriculture, industry, and defense. The field has seen rapid advances technology, but it is difficult gauge current capabilities identify gaps across projects. To this end, our foundry was assessed via a timed "pressure test", which 3 months were given build produce 10 molecules unknown us advance. By applying diversity of new approaches, we produced...
Article9 November 2017Open Access Transparent process Genetic circuit characterization and debugging using RNA-seq Thomas E Gorochowski orcid.org/0000-0003-1702-786X Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute Technology, Cambridge, MA, USA Search for more papers by this author Amin Espah Borujeni orcid.org/0000-0002-3036-7183 Yongjin Park Alec AK Nielsen Jing Zhang Bryan S Der D Benjamin Gordon Broad MIT Harvard, Christopher A Voigt Corresponding...
Synthetic genomics requires improved technologies
Malaria is a global infectious disease that threatens the lives of millions people. Transcriptomics, proteomics and functional genomics studies, as well sequencing Plasmodium falciparum Homo sapiens genomes, have shed new light on this host-parasite relationship. Recent advances in accurate mass measurement spectrometry, sophisticated data analysis software, availability biological pathway databases, converged to facilitate our global, untargeted biochemical profiling study vitro P....
Abstract Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as needle-like molecular machine. The many involved in T3SS construction are tightly regulated due to its role pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation simplified genetics by removing or recoding genes, scrambling gene order replacing all non-coding DNA synthetic genetic parts. This process...
DNAplotlib ( www.dnaplotlib.org ) is a computational toolkit for the programmable visualization of highly customizable, standards-compliant genetic designs. Functions are provided to aid with both tasks and extract overlay associated experimental data. High-quality output produced in form vector-based PDFs, rasterized images, animated movies. All aspects rendering process can be easily customized or extended by user cover new forms part regulation. supports improved communication design...
Genetic memory can be implemented using enzymes that catalyze DNA inversions, where each orientation corresponds to a "bit". Here, we use two invertases (FimE and HbiF) reorient irreversibly between states with opposite directionality. First, construct is set by FimE reset HbiF. Next, build NOT gate the input promoter drives in absence of signal reverse state maintained constitutive expression The requires ∼3 h turn on off. evolutionary stabilities these circuits are measured passaging cells...
Genetic designs can consist of dozens genes and hundreds genetic parts. After evaluating a design, it is desirable to implement changes without the cost burden starting construction process from scratch. Here, we report two-step where large design space divided into deep pools composite parts, which individuals are retrieved assembled build final construct. The built via multiplexed assembly sequenced using next-generation sequencing. Each pool consists ∼20 Mb up 5000 unique...
The radical S-adenosylmethionine (SAM) enzyme NifB occupies a central and essential position in nitrogenase biogenesis. catalyzes the formation of an [8Fe-9S-C] cluster, called NifB-co, which constitutes core active-site cofactors for all 3 types. Here, we produce functional aerobically cultured Saccharomyces cerevisiae Combinatorial pathway design was employed to construct 62 strains transcription units driving different expression levels mitochondria-targeted nif genes (nifUSXB fdxN) were...
Recently, semirational approaches that rely on combinatorial assembly of characterized DNA components have been used to engineer biosynthetic pathways. In practice, however, it is not practical assemble and test millions pathway variants in order elucidate how different affect the behavior a pathway. To address this challenge, we apply rigorous mathematical approach known as design experiments (DOE) can be construct empirical models system without testing all variants. support approach,...