A5008712496- Reproductive Biology and Fertility
- Ovarian function and disorders
- Reproductive Health and Technologies
- Sperm and Testicular Function
- Assisted Reproductive Technology and Twin Pregnancy
- Epigenetics and DNA Methylation
- Renal and related cancers
- Genetic Syndromes and Imprinting
- Bone and Dental Protein Studies
- Prenatal Screening and Diagnostics
- Eating Disorders and Behaviors
- Silk-based biomaterials and applications
- Radiative Heat Transfer Studies
- Fluid Dynamics and Turbulent Flows
- Turbomachinery Performance and Optimization
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Glycosylation and Glycoproteins Research
- Computational Fluid Dynamics and Aerodynamics
- Moringa oleifera research and applications
- Opioid Use Disorder Treatment
- Alkaloids: synthesis and pharmacology
- Central Venous Catheters and Hemodialysis
- Urban Agriculture and Sustainability
- Venous Thromboembolism Diagnosis and Management
Universidad Peruana Cayetano Heredia
2019-2023
Hospital Universitario Fundación Alcorcón
2005-2021
Centro Peruano de Estudios Sociales
2017-2020
Vrije Universiteit Brussel
2009-2020
New Mexico State University
2016-2019
Aalborg University
2017
Universitair Ziekenhuis Brussel
2009-2010
University of Chile
1990-1997
Instituto de Salud Pública de Chile
1997
Are meiotic and developmental competence of human oocytes from small (2-8 mm) antral follicles improved by applying an optimized IVM method involving a prematuration step in presence C-Type Natriuretic Peptide (CNP) followed maturation FSH Amphiregulin (AREG)?A strategy culture (PMC) the CNP using + AREG increases oocyte potential leading to higher availability Day 3 embryos good-quality blastocysts for single embryo transfer.IVM is minimal-stimulation ART with reduced hormone-related side...
Abstract Purpose Standard oocyte in vitro maturation (IVM) usually results lower pregnancy rates than fertilization (IVF). IVM preceded by a prematuration step improves the acquisition of developmental competence and can enhance embryo quality (EQ). This study evaluated effectiveness biphasic culture system incorporating steps (CAPA-IVM) versus standard women with polycystic ovarian morphology (PCOM). Methods Eighty (age < 38 years, ≥ 25 follicles 2–9 mm both ovaries, no major uterine...
Oncofertility focuses on providing fertility and endocrine-sparing options to patients who undergo life-preserving but gonadotoxic cancer treatment. The resources needed meet patient demand often are fragmented along disciplinary lines. We quantify assets gaps in oncofertility care a global scale.Survey-based questionnaires were provided 191 members of the Consortium Global Partners Network, National Institutes Health-funded organization. Responses analyzed measure trends regional subtleties...
Are oocyte size, chromatin remodelling, transcriptional activity and mitochondrial distribution in human immature oocytes from early antral follicles retrieved for vitro maturation (IVM) associated with the acquisition of meiotic competence? Oocyte compaction, cessation RNA synthesis mitochondria rearrangement around nucleus are oocyte's potential to resume meiosis vitro. Information on features that confer competence mainly derives germinal vesicle (GV) failed following an hCG trigger after...
Does in vitro maturation (IVM) of cumulus-enclosed germinal vesicle (GV) stage oocytes retrieved from small antral follicles minimally stimulated cycles without an ovulatory hCG dose induce imprinting errors at LIT1, SNRPN, PEG3 and GTL2 human oocytes? There is no significant increase mutations after IVM GV priming. Animal models have generally demonstrated correct methylation imprint establishment for grown matured oocytes. For IVM, well-designed studies allowing conclusions on are...
C-type natriuretic peptide (CNP) and its receptor 2 (NPR2) play a paramount role in the maintenance of oocyte meiotic arrest antral follicles via regulation intra-oocyte levels cyclic guanosine monophosphate adenosine monophosphate. We investigated potential CNP 1) to maintain during prolonged prematuration culture 2) sustain acquisition developmental competence immature cumulus-oocyte complexes (COCs). Compact COCs were collected from small prepubertal unprimed mice placed under different...
Does imprinted DNA methylation or gene expression differ between human blastocysts from conventional ovarian stimulation (COS) and an optimized two-step IVM method (CAPA-IVM) in age-matched polycystic ovary syndrome (PCOS) patients?No significant differences were detected COS CAPA-IVM blastocysts.Animal models have revealed alterations maintenance at germline differentially methylated regions (gDMRs) after use of ARTs. This effect increases as more ART interventions are applied to oocytes...
Imprinted genes are differentially methylated during gametogenesis to allow parental-specific monoallelic expression of genes. During mouse oogenesis, DNA methylation at imprinted is established the transition from primordial antral follicle stages. Studies in human and suggest aberrant imprinting oocytes following vitro maturation after superovulation with high doses gonadotrophines. The exact mechanisms leading unknown. We examined status regions key (by bisulphite sequencing) metaphase II...
Imprinted genes are differentially methylated during gametogenesis to allow parent-of-origin-specific monoallelic expression. We previously demonstrated establishment of normal imprinting at four key imprinted in mouse metaphase II oocytes after vitro follicle culture. Commercially available culture media feature a wide range methyl donor levels. The aim the present study was examine effect low (methionine, vitamin B(12), folic acid, choline, and B(6)) levels on acquisition DNA methylation...
Oocyte cytoplasmic maturation is influenced by the quantity of synthesized RNA and proteins accumulated stored during growth. Transcriptional repression degradation transcripts occur oocyte nuclear maturation, prolonged transcriptional arrest might compromise stores for early development. quantification key genes in oocytes be valuable when setting up vitro cultures that lack normal hormonal interplay found vivo. This study quantifies gene expression levels relation to follicle culture time...
Follicle-stimulating hormone (FSH) and oocyte-secreted factors influence granulosa cell differentiation follicle development. Whereas FSH stimulates the expression of mural transcripts, regulate specific cumulus genes suppress appearance transcripts. This study addresses extent to which clinically relevant changes in doses applied during antral development vitro could alter oocyte A 12-day culture system mouse ovarian preantral follicles can grow preovulatory was used. The following three...
Purpose In the accompanying article, “Survey of Fertility Preservation Options Available to Patients With Cancer Around Globe,” we showed that specific fertility preservation services may not be offered at various sites around world because cultural and legal barriers. We assessed global regional experiences as well status third-party reproduction adoption serve a comprehensive international data set resource for groups wish begin oncofertility interventions. Methods provide on legalities...
Gonadotropinsand insulin are major regulators of cell proliferation, differentiation, and survival in cultured mouse ovarian follicles. Applications variable doses combination with follicle-stimulating hormone (FSH) luteinizing (LH) were studied at the gene expression level oocytes cumulus cells. Early preantral follicles grown over 9 days sequentially exposed to combinations insulin, FSH, human chorionic gonadotropin (hCG). From culture Day 1 6 (preantral stage), two concentrations (5 ng/ml...
Abstract STUDY QUESTION Does use of medium containing amphiregulin improve meiotic maturation efficiency in oocytes women with polycystic ovary syndrome (PCOS) undergoing vitro (IVM) preceded by a capacitation culture step IVM (CAPA-IVM)? SUMMARY ANSWER Use significantly increased the rate from retrieved follicles diameters &lt;6 or ≥6 mm pre-cultured medium. WHAT IS KNOWN ALREADY Amphiregulin concentration follicular fluid is correlated human oocyte developmental competence. added to...
The state of limited resource settings that Coronavirus (COVID-19) pandemic has created globally should be taken seriously into account especially in healthcare sector. In oncofertility, patients receive their fertility preservation treatments urgently even before initiation anticancer therapy. Therefore, it is very crucial to learn more about oncofertility practice such as developing countries suffer often from shortage services provided young with cancer. As an extrapolation during the...
Imprinted genes are differentially methylated during gametogenesis to allow parent-of-origin-specific monoallelic expression. Follicle culture under oil overlay has been associated with altered imprinting establishment in mouse oocytes. We previously demonstrated normal at four key imprinted oocytes grown and matured a long-term vitro follicle system without overlay. Ammonium (300 microM) linked aberrant preimplantation embryo culture. Compared oil, mineral led dramatic increase ammonia...
Little is known about oncofertility practice in developing countries that usually suffer from a shortage of health services, especially those related to cancer care.
Highly-loaded low-pressure turbine blades promise overall performance improvements and lower costs but suffer from unacceptable endwall losses. The flow physics have to better understood develop effective control strategies aimed at a reduction of the Air Force Research Laboratory is carrying out highly resolved particle image velocimetry measurements oil-flow visualizations for L2F airfoil Re=100,000. To complement wind tunnel experiments, implicit large-eddy simulations same are being...
Objective: To evaluate the impact of patient follicular environment with oxidative stress on oocyte quality.Methods: Patients fertility treatment either advanced maternal age or endometriosis were asked to donate fluid collected during ovum pick-up.Follicular (FF) was added (20%, 10% and 5%; %V/V) in vitro maturation (IVM) medium mouse oocytes.Following culture, oocytes assessed for meiosis reinitiation.In a second setup, coenzyme Q10 culture FF.In addition assessing meiotic maturation,...
The cytoprotective and anti-inflammatory effects of boldine in an experimental model acute colitis are reported. administration to animals with induced by the intrarectal acetic acid, was found protect against colonic damage as expressed major reductions extent cell death, tissue disorganization, edema. Boldine also reduced neutrophil infiltration, measured myeloperoxidase activity, but it did not significantly affect lipoperoxides. preserve fluid transport, a function otherwise markedly...