A5028157551- Plant biochemistry and biosynthesis
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Sirtuins and Resveratrol in Medicine
- Traditional Chinese Medicine Analysis
- Pharmacological Effects of Natural Compounds
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Porphyrin Metabolism and Disorders
- Enzyme-mediated dye degradation
- Biochemical and Molecular Research
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Advanced Electron Microscopy Techniques and Applications
Graz University of Technology
2020-2024
Institute of Molecular Biotechnology
2020-2023
Pontificia Universidad Católica de Chile
2018-2023
BioTechMed-Graz
2023
Cold-active enzymes maintain a large part of their optimal activity at low temperatures. Therefore, they can be used to avoid side reactions and preserve heat-sensitive compounds. Baeyer-Villiger monooxygenases (BVMO) utilize molecular oxygen as co-substrate catalyze widely employed for steroid, agrochemical, antibiotic, pheromone production. Oxygen has been described the rate-limiting factor some BVMO applications, thereby hindering efficient utilization. Considering that solubility in...
Pinostilbene is a monomethyl ether analog of the well-known nutraceutical resveratrol. Both compounds have health-promoting properties, but latter undergoes rapid metabolization and has low bioavailability. O-methylation improves stability bioavailability In plants, these reactions are performed by O-methyltransferases (OMTs). Few efficient OMTs that monomethylate resveratrol to yield pinostilbene been described so far. Here, we report engineering OMT from Vitis vinifera (VvROMT), which...
The development of sustainable processes for the valorization byproducts and other waste streams remains an ongoing challenge in field catalysis. Racemic borneol, isoborneol camphor are currently produced from α-pinene, a side product production cellulose. pure enantiomers these monoterpenoids have numerous applications cosmetics act as reagents asymmetric synthesis, making enzymatic route their separation into optically desirable goal. Known short-chain borneol-type dehydrogenases (BDHs)...
Enzymatic decarboxylation of phenolic acids enables the production biobased styrenes under mild reaction conditions. However, free para-phenolic group can lead to undesirable side products during polymerization, giving protection OH critical importance for application in adhesives. Here we present a one-pot two-step cascade reaction, which acid decarboxylase from Bacillus subtilis (BsPAD) catalyzes coumaric acid, caffeic ferulic and sinapic followed by O-methylation intermediate resveratrol...
Enzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large diverse pools of biosynthetic enzymes that facilitate complex reactions, such formation intricate terpene carbon skeletons, with exquisite specificity. High-resolution structural analysis these is crucial order to understand their mechanisms modulate properties by targeted engineering. Although cryo-electron...
ABSTRACT Enzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large diverse pools of biosynthetic enzymes that facilitate complex reactions, such formation intricate terpene carbon skeletons, with exquisite specificity. High-resolution structural analysis these is crucial to understand their mechanisms modulate properties by targeted engineering. Although cryo-electron...