We report a new methodology for direct visualization of superoxide production in the dopaminergic area brain Parkinson's disease, based on redox cycle mito-TEMPO, blood-brain barrier-, cell-, and mitochondria-penetrating nitroxide derivative with scavenging properties T1 magnetic resonance imaging (MRI) contrast. The experiments were conducted healthy 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. In mice, nitroxide-enhanced MRI signal was weak short-lived (half-life ∼ 40...

Glioblastoma is one of the most aggressive brain tumors, characterized by a pronounced redox imbalance, expressed in high oxidative capacity cancer cells due to their elevated glycolytic and mitochondrial metabolism. The assessment modulation state glioblastoma are crucial factors that can provide highly specific targeting treatment. Our study describes pharmacological strategy for using redox-active combination drug. experiments were conducted vivo on mice (intracranial model) vitro cell...

Lecithinized superoxide dismutase (PC-SOD) was found to have a significantly improved half-life in the bloodstream and better pharmacological effects compared with unmodified SOD. However, there is no direct evidence that parenterally administered PC-SOD decreases levels blood tissues vivo. In present study, we investigated ability of versus SOD as scavenger mice subjected oxidative stress. Experiments were performed on lipopolysaccharide (LPS) mouse model acute inflammation known be...

Redox-active substances and their combinations, such as of quinone/ascorbate in particular menadione/ascorbate (M/A; also named Apatone®), attract attention with unusual ability to kill cancer cells without affecting the viability normal well synergistic anticancer effect both molecules. So far, primary mechanism M/A-mediated effects has not been linked mitochondria. The aim our study was clarify whether this “combination drug” affects mitochondrial functionality specifically cells. Studies...

Our study proposes a pharmacological strategy to target cancerous mitochondria via redox-cycling "mitocans" such as quinone/ascorbate (Q/A) redox-pairs, which makes cancer cells fragile and sensitive without adverse effects on normal tissues. Eleven Q/A redox-pairs were tested cultured cancer-bearing mice. The following parameters analyzed: cell proliferation/viability, mitochondrial superoxide, steady-state ATP, tissue redox-state, tumor-associated NADH oxidase (tNOX) expression, tumor...

Aesthetic physicians have several hundred injectable products to select from. Due differences in their manufacturing technology, these display varying biophysical qualities, such as cohesivity and lift capacity. Currently, there is no guidance objectively selecting the best product for a particular patient. Therefore, an algorithmic approach required take specific skin characteristics into consideration.To evaluate (1) whether subjects seeking treatments midfacial volume loss and/or contour...

The present study was designed to investigate whether poly-ion complex hollow vesicles (polymersomes), based on chemically modified chitosan, are appropriate for passive tumour targeting in the context of their application as drug carriers. experiments were performed colon cancer-grafted mice. mice subjected anaesthesia and injected intravenously with water-soluble nanoparticles: (1) QD705-labelled polymersomes (average size ∼120 nm; distribution ∼10%) or (2) native QD705. optical imaging...

Total redox capacity (TRC) and oxidative stress (OxiStress) of biological objects (such as cells, tissues, body fluids) are some the most frequently analyzed parameters in life science. Development highly sensitive molecular probes analytical methods for detection these is a rapidly growing sector BioTech's R&D industry. The aim present study was to develop quantum sensors tracking TRC and/or OxiStress living using electron-paramagnetic resonance (EPR), magnetic imaging (MRI), optical...

Background/Aim: Recent studies provided convincing evidence for the anticancer activity of combined application vitamin C and pro-vitamin K3 (menadione). The molecular pathways underlying this process are still not well established. present study aimed to investigate effect combination plus on redox status leukemia normal lymphocytes, as their sensitizing a variety drugs. Materials Methods: Cytotoxicity substances was analyzed by trypan blue staining automated counting live dead cells....

Optimizing outcomes of aesthetic treatments with injectable products usually requires a consideration the entire face to ensure balance, along combination that align patient's goals. To help injectors, method assessing patient and developing an individualized, holistic treatment plan was developed. This methodology is termed Assessment, Anatomy, Range, Treatment (AART™) Holistic Individualized Treatments (HITs™). article aims describe evaluate novel systematic AART-HIT™ methodology.

Background/Aim: The aim of this study was to elucidate the possibility sensitizing colon cancer cells chemotherapeutic drug SN38 and investigate its mechanism action after combined treatment with electroporation (EP). Materials Methods: Cells were treated SN38, EP their combination for 24/48 h. cell viability, actin cytoskeleton integrity, mitochondrial superoxide, hydroperoxides, total glutathione, phosphatidyl serine expression, DNA damages expression membrane ABC transporters analyzed...

The present study was designed to investigate whether poly-ion complex hollow vesicles (polymersomes), based on chemically-modified chitosan, are appropriate for lymph node mapping in the context of their application development theranostic nanosized drug delivery systems (nano-DDS). experiments were performed Balb/c nude mice (colon cancer-grafted). subjected anesthesia and quantum dot (QD(705))-labeled polymersomes (d-120 nm) injected intravenously via tail vein. optical imaging carried...

The effect of UV-B irradiation at temperatures 22 and 4 °C on flash induced oxygen yields, photochemical activity, energy transfer in pea thylakoid membranes the absence presence scavengers reactive species (ROS) was studied. Three different were used: dimethyl sulfoxide (DMSO), histidine (His), n-propyl gallate (nPG). As result treatment isolated membranes, yields considerably affected - amplitudes decreased oscillation pattern lost. analysis initial burst showed alterations a number...

The effect of high temperature (HT) and dehydration on the activity photosynthetic apparatus its ability to restore membrane properties, oxygen evolution, energy distribution upon rehydration were investigated in a resurrection plant, Haberlea rhodopensis. Plants growing under low irradiance their natural habitat desiccated air-dry state at similar light intensity [about 30 μol(photon) m-2 s-1] optimal day/night (23/20°C) or (38/30°C) temperature. Our results showed that HT alone reduced...

Objectives: The present study describes a pharmacological strategy for the treatment of glioblastoma by redoxcycling 'mitocans' such as quinone/ascorbate combination drugs, based on their tumor-selective redox-modulating effects and tolerance to normal cells tissues.Methods: Experiments were performed mice (orthotopic model) treated with coenzyme Q0/ascorbate (Q0/A). drug was injected intracranially in single dose. following parameters analyzed vivo using MRI orex conventional assays: tumor...

This study reports a non-invasive magnetic resonance imaging (MRI) of kidney dysfunction in mice, based on the induction redox-imbalance and oxidative stress renal tissues, using mito-TEMPO as redox-sensitive contrast probe. Kidney was triggered by hypercholesterolemia. The mice were divided three groups: (i) normal diet (ND); (ii) cholesterol (CD); (iii) plus cholestyramine (CC). After 15 weeks feeding, subjected to following analyses: plasma levels; serum test for functionality;...

We describe a pharmacological strategy for selectively targeting glioblastoma using redox-active combination drug menadione/ascorbate (M/A), compared to the chemotherapeutic standard-of-care temozolomide (TMZ).Experiments were conducted on mice (GS9L cell transplants - intracranial model), treated with M/A or TMZ. Tumor growth was monitored by magnetic resonance imaging. Effects of and TMZ viability overproduction mitochondrial superoxide also evaluated isolated cells (GS9L) normal...

The present study was directed to the development of EPR methodology for distinguishing cells with different proliferative activities, using “redox imaging.” Three nitroxide radicals were used as redox sensors: (a) mito-TEMPO—cell-penetrating and localized mainly in mitochondria; (b) methoxy-TEMPO—cell-penetrating randomly distributed between cytoplasm intracellular organelles; (c) carboxy-PROXYL—nonpenetrating living evenly extracellular environment. experiments conducted on eleven cell...

We used a mitochondria-penetrating nitroxide, mito-TEMPO, as contrast probe for imaging of kidney dysfunction in mice, based on the redox-imbalance and oxidative stress renal tissues. Kidney was triggered by hypercholesterolemia. The mice were divided three groups: (i) normal diet (ND; control); (ii) cholesterol (CD); (iii) plus cholestyramine (CC). CD showed increased plasma levels total non-HDL-cholesterol, well serum blood urea nitrogen, uric acid creatinine, compared to ND mice. CC...