Transcription factor AP-1 transduces environmental signals to the transcriptional machinery. To ensure a quick response yet maintain tight control over target genes, activity is likely be negatively regulated in nonstimulated cells. identify proteins that interact with Jun subunits of and repress its activity, we developed novel screen for detecting protein-protein interactions not based on readout. In this system, mammalian guanyl nucleotide exchange (GEF) Sos recruited Saccharomyces...

From a mouse genomic library, clone has been isolated that codes for connexin-homologous sequence of 358 amino acids. Because its theoretical molecular mass 40.418 kD it is named connexin40 (Cx40). Based on both protein and nucleotide sequence, Cx40 more closely related to Cx43 (alpha subgroup connexins) than Cx32 (beta subgroup). The highest overall homology detected, however, was chick Cx42 (67% acid 86% identity), raising the possibility may be analogue. coding region uninterrupted by...

The coding sequence (333 amino acids) of a new connexin protein, designated mouse connexin37 (Cx37 or Cx37.6) due to the deduced theoretical molecular mass 37.600 kD, has been determined from cDNA and genomic clones. As seen in other connexins, its gene no introns within region acid is predicted have similar topology connexins that form intercellular channels. Cx37 most rat connexin43 (59% identity) Xenopus connexin38 (66% when compared NH2 terminus end fourth putative transmembrane region....

We have identified CALNUC, an EF-hand, Ca2+-binding protein, as a Golgi resident protein. CALNUC corresponds to previously EF-hand/calcium-binding protein known nucleobindin. interacts with Gαi3 subunits in the yeast two-hybrid system and GST-CALNUC pull-down assays. Analysis of deletion mutants demonstrated that EF-hand intervening acidic regions are site CALNUC's interaction Gαi3. is found both cytosolic membrane fractions. The pool tightly associated luminal surface membranes. widely...

The Pim-1 oncogene encodes a serine-threonine kinase that relays signals from cytokine receptors and contributes to the formation of lymphoid tumors when expressed at high levels. Here we show protein Cdc25 C-associated 1 (C-TAK1) is binding partner substrate Pim-1. A physical interaction C-TAK1 could be shown biochemically in yeast two-hybrid assays. Immunofluorescence experiments suggested Pim-1.C-TAK1 complexes are predominantly cytoplasmic. When transiently transfected, was also found...

Two new gap junction genes isolated from the mouse genome code for connexin homologues of 271 and 266 amino acids, designated here Cx31.1 Cx30.3, respectively. The two open reading frames, oriented in same direction, are only 3.4 kb apart on chromosome 4. Within family, these proteins most closely related to one another (70% acid sequence identity) Cx31 (65 68% identity, respectively). Comparison gene with a cDNA showed similar genomic organization that found other members i.e. coding...

GHF-1 is a member of the POU family homeodomain proteins. It cell-type-specific transcription factor responsible for determination and expansion growth hormone (GH)- prolactin-expressing cells in anterior pituitary. was previously suggested that cyclic AMP (cAMP)-responsive protein kinase A (PKA) phosphorylates at site within N-terminal arm its homeodomain, thereby inhibiting binding to GH promoter. These results, however, are inconsistent with physiological stimulation production by cAMP...

SSTR5 physically interacts with ZDHHC5 by pull down (View interaction) Palmitoylation increases the hydrophobicity of protein domains, thus contributing to structure, function and cellular localization [1]. In case G-protein coupled receptors (GPCRs), palmitoylation a cysteine residue in intracellular C-terminal tail creates an additional loop [2] which is important for efficient coupling G-proteins [3, 4]. addition, it believed that are targeted lipid rafts this modification [5]. Though...

We have used the Ras recruitment system to screen for proteins that interact with N-terminally located transactivation domain of c-Myc. The is based on activation mitogenic RAS signaling pathway in yeast by mammalian GTPase Ha-Ras. This led identification two novel nuclear termed Krim-1A and Krim-1B both contain an N-terminal KRAB box 12 or 9 Krüppel C2H2 type zinc fingers at C terminus, respectively. found sequences covering Myc II homology region are essential interaction Krim-1 second...

The Pax-6 gene encodes a transcriptional master regulator involved in the development of eye. quail is expressed neuroretina from two promoters, P0 and P1, regulated by an intragenic neuroretina-specific enhancer (EP enhancer). activity this restricted to promoter, which activated at onset neuronal differentiation. In article, we show that POU domain transcription factor Brn-3b, various regions brain including retina sensory neurons, one factors interacting with EP enhancer. Brn-3b strongly...

Abstract The fast paced progress in genomics yielded a multitude of novel identified genes. Therefore reliable and up scalable methods are required to identify the functions encoded proteins. By using protein‐protein interaction screening technologies, function many proteins has successfully been assigned, proved combination with other techniques. We describe application an vivo system for interactions, Ras‐recruitment (RRS). RRS used extensively partners, also cases where common systems...

Abstract Direct targeting of several important cancer pathways has so far proved challenging, owing to the lack pathway-specific targets that can be accessed by conventional small molecule drugs or therapeutic antibodies. Nexigen develops and advances peptide-based therapeutics, a new class functionally modulate currently undruggable intracellular protein-protein-interactions. One these pathways, Wnt signaling cascade, been identified as key pathway in stem cells maintenance. Targeting...