A5034629143- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Photoreceptor and optogenetics research
- Myasthenia Gravis and Thymoma
- Algal biology and biofuel production
- Peripheral Neuropathies and Disorders
- Antifungal resistance and susceptibility
- Spectroscopy and Quantum Chemical Studies
- Molecular spectroscopy and chirality
- Ion channel regulation and function
- Eicosanoids and Hypertension Pharmacology
- Hormonal Regulation and Hypertension
- Muscle Physiology and Disorders
- Plant and animal studies
- Organometallic Complex Synthesis and Catalysis
- Estrogen and related hormone effects
- Effects and risks of endocrine disrupting chemicals
- Biocrusts and Microbial Ecology
- Botulinum Toxin and Related Neurological Disorders
- Plant Molecular Biology Research
- Reproductive Health and Contraception
- Thyroid and Parathyroid Surgery
- Viral Infectious Diseases and Gene Expression in Insects
- Pharmacogenetics and Drug Metabolism
- Radiopharmaceutical Chemistry and Applications
Argenx (Belgium)
2024-2025
Ablynx (Belgium)
2024
Grünenthal Group (Germany)
2006-2022
Max Planck Society
1997-2004
Technische Universität Berlin
2004
Freie Universität Berlin
1997
The photoconversion of phytochrome (phytochrome A from Avena satina) the inactive (Pr) to physiologically active form (Pfr) was studied by near-infrared Fourier transform resonance Raman spectroscopy at cryogenic temperatures, which allow us trap intermediate states. Nondeuterated and deuterated buffer solutions were used determine effect H/D exchange on spectra. For first time, reliable spectra "bleached" intermediates meta-RA meta-RC obtained. vibrational bands in region 1300−1700 cm-1, is...
The new analgesic tapentadol was evaluated for induction and inhibition of several cytochrome P450 enzymes in vitro, protein binding assessed. It concluded that no clinically relevant drug-drug interactions are likely to occur through either mechanism.
The chromophore structures in the parent states Pr and Pfr as well photocycle intermediate Lumi-R of oat phytochrome phyA are determined by comparison experimental resonance Raman spectra with calculated that have been obtained density functional theory calculations (B3LYP) using scaled force fields. were for various tetrapyrrole geometries including more than twenty different methine bridge isomers. For best agreement terms vibrational frequencies, isotopic shifts, intensities was achieved...
A phytochrome-encoding cDNA from the cyanobacterium Synechocystis has been heterologously expressed in Escherichia coli and reconstituted into functional chromoproteins by incubation with either phycocyanobilin (PCB) or phytochromobilin (PΦB). These materials were studied Raman spectroscopy nanosecond flash photolysis. The spectra suggest far-reaching similarities chromophore configuration conformation between Pfr forms of phytochrome plant phytochromes (e.g. phyA oat), but some differences,...
Muscle-specific kinase (MuSK) is essential for the formation, function, and preservation of neuromuscular synapses. Activation MuSK by a agonist antibody may stabilize or improve function junction (NMJ) in patients with disorders NMJ, such as congenital myasthenia (CM). Here, we generated characterized ARGX-119, first-in-class humanized monoclonal specific MuSK, that being developed treatment diseases. We performed vitro ligand-binding assays to show ARGX-119 binds high affinity...
The isolated α-subunits of the light-harvesting pigments phycoerythrocyanin (PEC) and C-phycocyanin (CPC) Mastigocladus laminosus were studied by resonance Raman (RR) spectroscopy. results for PEC indicate that photoconversion tetrapyrrole chromophore from Z,Z to Z,E configuration is associated with structural changes which are largely restricted photoisomerization site, i.e. methine-bridge C–D. Evidently rotation around this double bond not complete in first intermediate can be detected...
The recombinant 65‐kDa polypeptide of phyA oat phytochrome was expressed by yeast Pichia pastoris and assembled into two chromopeptides with the chromophores phytochromobilin (PΦB) phycocyanobilin (PCB), respectively. P r fr states protein variants were characterized resonance Raman (RR) spectroscopy compared native demonstrating that deletion C‐terminal half does not alter structure chromophore site within N‐terminal half. Most RR spectral changes observed upon replacing PΦB PCB can be...
Phytochromes are photoreceptors that upon light absorption initiate a physiological reaction cascade. The starting point is the photoisomerization of tetrapyrrole cofactor in parent Pr state, followed by thermal relaxation steps culminating activation signal. Here we have employed resonance Raman (RR) spectroscopy to study chromophore structure primary photoproduct Lumi-R, trapped between 130 and 200 K. investigations covered phytochromes from plants (phyA) prokaryotes (Cph1, Agp1, CphB,...
Abstract ARGX-119 is a novel, humanized, agonist monoclonal SIMPLE Antibody™ specific for muscle-specific kinase (MuSK) that being developed treatment of patients with neuromuscular diseases. the first antibody (mAb) binds high affinity to Frizzled-like domain human, non-human primate, rat and mouse MuSK, without off-target binding, making it suitable clinical development. Within Fc-region, harbors L234A, L235A mutations diminish potential immune-activating effector functions. Its...
By the reaction of AuI with alkali metal hydrogen acetylides MIC2H (MI = Li–Cs) in liquid ammonia and subsequent heating remaining residue refluxing pyridine Li, Na, K) or as a solid phase at about 110 °C vacuum Rb, Cs) ternary gold MIAuC2 were obtained. Their crystal structures investigated by means X-ray powder diffraction. [Au(C2)2/2–] chains are characteristic structural motif which packed hexagonal (LiAgC2) tetragonal arrangement (NaAuC2–CsAuC2), respectively. Simple calculations based...
In vitro binding tests to human receptors and in vivo functional activities animals were used compare the effects of progestin chlormadinone acetate (CMA) its 3alpha- 3beta-hydroxy metabolites (3alpha-OH-CMA 3beta-OH-CMA) on progesterone, androgen glucocorticoid receptors.CMA, 3alpha-OH-CMA, 3beta-OH-CMA reference R5020 bound progesterone receptor with Ki values 2.5 nm, 13 6.0 nm 4.3 respectively. Binding affinities characterized by 3.8 nM for CMA, 83 20 2.9 methyltrienolone. The 16 69 21...
The 39‐kDa fragment of oat phytochrome phyA , obtained by tryptic digestion at the amino acids 65 and 425, was studied resonance Raman spectroscopy. parent state P r reveals far‐reaching similarities with that native implying structures tetrapyrrole chromophore its immediate protein environment are not affected proteolysis. However, spectrum final product phototransformation, denoted as bl is more closely related to fr precursor phytochrome, i.e. meta‐R C rather than itself. spectra indicate...
Abstract Muscle-specific kinase (MuSK) orchestrates establishment and maintenance of neuromuscular synapses, which enable muscle contraction. Autoantibodies targeting MuSK cause myasthenia gravis (MG), a disease characterized by fatigable skeletal weakness requires chronic immunosuppressive treatment ventilatory support at some point in ∼30% patients. autoantibodies are predominantly IgG4 bispecific, functionally monovalent antibodies due to Fab-arm exchange. Through binding, act as...