A5100660490- Pluripotent Stem Cells Research
- Graphene research and applications
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- 2D Materials and Applications
- Ga2O3 and related materials
- DNA Repair Mechanisms
- RNA Research and Splicing
- Nanoplatforms for cancer theranostics
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- MXene and MAX Phase Materials
- Cancer Research and Treatments
Nanjing University
2017-2023
Collaborative Innovation Center of Advanced Microstructures
2023
Model Animal Research Center
2017-2020
Pharmaceutical Biotechnology (Czechia)
2018
Abstract 2D semiconductors, especially transition metal dichalcogenides (TMDCs), have attracted ever‐growing attention toward extending Moore's law beyond silicon. Metal–organic chemical vapor deposition (MOCVD) has been widely considered as a scalable technique to achieve wafer‐scale TMDC films for applications. However, current MOCVD process usually suffers from small domain size with only hundreds of nanometers, in which dense grain boundary defects degrade the crystalline quality films....
Polycomb group (PcG) proteins are epigenetic transcriptional repressors that orchestrate numerous developmental processes and have been implicated in the maintenance of embryonic stem (ES) cell state. More recent evidence suggests a subset PcG engages activation some cellular contexts, but how this property is exerted remains largely unknown. Here, we generated ES cells with single or combined disruption polycomb RING finger protein 3 (Pcgf3) Pcgf5 CRISPR-Cas9 technique. We report although...
Highlights•The methyl-lysine-binding aromatic cage is dispensable for L3mbtl2 chromatin targeting•The conserved Pho-binding pocket critical functions•L3mbtl2 assembles a complex whose composition with the Drosophila PhoRC•Targeted knockouts of L3mbtl2, Pcgf6, and Max compromise PRC1.6 occupancySummaryThough genetic data suggest that Polycomb group proteins (PcGs) are central modifiers repressors have been implicated in control embryonic stem cell (ESC) pluripotency, precise mechanism PcG...
Polycomb group (PcG) proteins are essential for maintenance of lineage fidelity by coordinating developmental gene expression programs. ring finger 6 (PCGF6) has been previously reported to repress lineage-specific genes, especially germ cell–related genes in mouse embryonic stem cells (ESCs) via the noncanonical polycomb repressive complex PRC1.6. However, molecular mechanism this repression remains largely unknown. Here, using RNA-Seq, real-time RT-PCR, immunohistochemistry,...
The polycomb group (PcG) proteins are key epigenetic regulators in stem cell maintenance. PcG have been thought to act through one of two repressive complexes (PRCs), but more recent biochemical analyses challenged this model the identification noncanonical PRC1 (nc-PRC1) characterized by presence Rybp or Yaf2 place canonical Chromobox proteins. However, biological significance these nc-PRC1s and potential mechanisms which they mediate gene repression largely unknown. Here, we explore...