A5102953947- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- Chronic Lymphocytic Leukemia Research
- Lung Cancer Research Studies
- Childhood Cancer Survivors' Quality of Life
- Congenital Diaphragmatic Hernia Studies
- Cancer therapeutics and mechanisms
- Genomic variations and chromosomal abnormalities
- Peptidase Inhibition and Analysis
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Adolescent and Pediatric Healthcare
- Viral Infectious Diseases and Gene Expression in Insects
- Lung Cancer Treatments and Mutations
- CAR-T cell therapy research
- Neonatal Respiratory Health Research
- Congenital Anomalies and Fetal Surgery
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Chromatin Remodeling and Cancer
- Cytomegalovirus and herpesvirus research
- Platelet Disorders and Treatments
- Autism Spectrum Disorder Research
- Genetics, Bioinformatics, and Biomedical Research
- Birth, Development, and Health
University of Yamanashi
2016-2024
Yamanashi Prefectural Central Hospital
2021-2024
Takeda (Japan)
2024
University of Yamanashi Hospital
2020
Tottori University
1998-2003
Japan Science and Technology Agency
2001
In patent ductus arteriosus (PDA) in preterm infants, the relationship between treatment timing and long-term developmental prognosis remains unclear. The purpose of this study was to clarify age days when closure occurred development. Preterm infants with a birth weight less than 1500 g who were admitted our NICU over period 9 years (2011–2019) diagnosed PDA included. A new version K-type test for corrected ages 1.5 3 used as an index duration evaluated using Pearson’s correlation...
Prognosis of childhood acute lymphoblastic leukemia (ALL) has been dramatically improved. However, prognosis the cases refractory to primary therapy is still poor. Recent phase 2 study on efficacy combination chemotherapy with bortezomib (BTZ), a proteasome inhibitor, for ALL demonstrated favorable clinical outcomes. septic death was observed in over 10% patients, indicating necessity biomarkers that could predict BTZ sensitivity. We investigated vitro sensitivity large panel cell lines...
Truncation of human chromosomes at desired sites by homologous recombination techniques enables functional and structural analyses development artificial chromosomes. However, this targeted truncation has been inefficient. We describe here an efficient method for in the chicken DT40 cells with a high rate. The chromosome 22 was transferred into cells, where telomeric repeat (TTAGGG)n to LIF locus on chromosome. Molecular cytogenetic showed that predicted occurred all clones.
In many cancers, somatic mutations confer tumorigenesis and drug-resistance. The recently established clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a potentially elegant approach to functionally evaluate in cancers. To reproduce by homologous recombination (HR), the HR pathway must be functional, but DNA damage repair frequently impaired Imatinib tyrosine kinase inhibitor for BCR-ABL1 Philadelphia chromosome-positive (Ph+) leukemia, development of...
Abstract Asparaginase therapy is a key component of chemotherapy for patients with T-cell acute lymphoblastic leukemia (T-ALL). depletes serum asparagine by deamination into aspartic acid. Normal hematopoietic cells can survive due to synthetase (ASNS) activity, whereas are supposed undergo apoptosis silencing the ASNS gene. Because gene has typical CpG island in its promoter, methylation status T-ALL may be associated asparaginase sensitivity. Thus, we investigated significance sensitivity...
Abstract Cytosine arabinoside (Ara‐C) is one of the key drugs for treatment acute myeloid leukemia. It also used consolidation therapy lymphoblastic leukemia (ALL). Ara‐C a deoxyadenosine analog and phosphorylated to form cytosine triphosphate (Ara‐CTP) as an active form. In first step metabolic pathway, Ara‐CMP by deoxycytidine kinase (DCK). However, current cumulative evidence in association sensitivity ALL appears inconclusive. We analyzed various cell lines possible involvement DCK...
Abstract t(17;19)(q21‐q22;p13), responsible for TCF3‐HLF fusion, is a rare translocation in childhood B‐cell precursor acute lymphoblastic leukemia(BCP‐ALL). t(1;19)(q23;p13), producing TCF3‐PBX1 common BCP‐ALL. Prognosis of t(17;19)‐ALL extremely poor, while that t(1;19)‐ALL has recently improved dramatically intensified chemotherapy. In this study, mRNA was detectable at high level during induction therapy newly diagnosed case, undetectable the end most cases. Using 4 and 16 cell lines,...
Abstract Glucocorticoid (GC) shows antileukaemic activity via binding to the GC receptor (GR). The human GR gene has 4 splicing variants besides functional isoform GRα, but their significance in sensitivity of acute lymphoblastic leukaemia (ALL) been inconsistent. Additionally, several studies evaluated relevance single nucleotide polymorphisms (SNPs) ALL, current cumulative evidence appears inconclusive. Addressing limitations previous studies, we used a large series B‐cell precursor ALL...
Abstract Identification of genetic variants associated with glucocorticoids (GC) sensitivity leukaemia cells may provide insight into potential drug targets and tailored therapy. In the present study, within 72 leukaemic cell lines derived from Japanese patients B‐cell precursor acute lymphoblastic (ALL), we conducted genome‐wide genotyping single nucleotide polymorphisms (SNP) attempted to identify GC NR3C1 (GC receptor) gene expression. IC50 measures for prednisolone (Pred) dexamethasone...
Abstract In chemotherapy for childhood acute lymphoblastic leukaemia (ALL), maintenance therapy consisting of oral daily mercaptopurine and weekly methotrexate is important. NUDT15 variant genotype reportedly highly associated with severe myelosuppression during therapy, particularly in Asian Hispanic populations. It has also been demonstrated that acquired somatic mutations the NT5C2 PRPS1 genes, which are involved thiopurine metabolism, detectable a portion relapsed ALL. To directly...
Abstract Background Several reports have documented that the pulmonary sequestration is in communication with gastrointestinal tract and concept of bronchopulmonary foregut malformation (BPFM) has become more widespread. However, there are few associated pancreas derived from foregut. We describe history pathophysiology BPFM including pancreatic tissue a male infant respiratory distress. Case presentation A patient was born at 38 weeks gestation weighed 2752 g birth. He developed pneumonia...
Abstract Background The genetic variants of the ARID5B gene have recently been reported to be associated with disease susceptibility and treatment outcome in childhood acute lymphoblastic leukemia (ALL). However, few studies explored association sensitivities chemotherapeutic agents. Methods We genotyped susceptibility-linked rs7923074 rs10821936 as well relapse-linked rs4948488, rs2893881, rs6479778 ARDI5B by direct sequencing polymerase chain reaction (PCR) products 72 B-cell precursor-ALL...
Recent advances in molecular and genetic approaches have identified a number of genes responsible for Noonan syndrome (NS). However, there has been limited analysis the genotype-phenotype correlation NS patients. Here, we report case Japanese patient with possessing c.853T>C (p.Phe285Leu) mutation gene encoding protein-tyrosine phosphatase, nonreceptor-type 11 (PTPN11). To clarify correlations, accumulation data on clinical course patients genetically confirmed is important. We summarized...
We encountered an extremely low birth weight infant with breast milk-transmitted cytomegalovirus (CMV) infection. To determine the transmission route, we conducted direct sequence analysis of two variable CMV genes, UL139, and UL146. When utilizing milk, possibility acquired infection should be considered tested for prompt diagnosis treatment.
Gastroschisis is a rare congenital anomaly in which abdominal organs herniate through defect the wall. Managing gastroschisis extremely low birth weight (ELBW) infants presents significant challenges because of their immature physiologies and increased risk complications.
The gastrointestinal (GI) tract is a common target organ of graft-vs-host disease (GVHD) in hematopoietic stem cell transplantation (HSCT) patients, and GI GVHD often resistant to standard treatments such as corticosteroids. Moreover, longterm use systemic corticosteroids sometimes induces adverse events infection. Beclomethasone dipropionate (BDP) potent, topically active corticosteroid, which metabolized an derivative the intestinal mucosa. Oral BDP therapy reportedly effective against...