A5024795697- Veterinary Equine Medical Research
- Animal health and immunology
- Reproductive Physiology in Livestock
- Veterinary Orthopedics and Neurology
- Microbial infections and disease research
- Animal Behavior and Welfare Studies
- Genetic and phenotypic traits in livestock
- Immunotherapy and Immune Responses
- Ruminant Nutrition and Digestive Physiology
- Veterinary Medicine and Surgery
- T-cell and B-cell Immunology
- Bone fractures and treatments
- Hip disorders and treatments
- Orthopaedic implants and arthroplasty
- Veterinary Pharmacology and Anesthesia
- Orthopedic Infections and Treatments
- Immune Cell Function and Interaction
- BRCA gene mutations in cancer
- Orthopedic Surgery and Rehabilitation
- Heart Rate Variability and Autonomic Control
- Tendon Structure and Treatment
- Bone Tissue Engineering Materials
- Agriculture and Farm Safety
- Hernia repair and management
- Effects of Environmental Stressors on Livestock
University of Tennessee at Knoxville
2016-2025
Knoxville College
2016-2025
King's College London
2024
House of Representatives
2024
Children's Center
2022
Eastern Michigan University
2009-2022
University of New Mexico
2022
Goryeb Children's Hospital
2022
Morristown Medical Center
2022
Boston University
2022
CD4+ T helper 1 (TH1) cells are important mediators of inflammation and regulated by numerous pathways, including the negative immune receptor Tim-3. We found that Tim-3 is constitutively expressed on innate system in both mice humans, it can synergize with Toll-like receptors. Moreover, an antibody agonist acted as adjuvant during induced responses, ligation distinct signaling events dendritic cells; latter finding could explain apparent divergent functions these cell types. Thus, virtue...
Linkage analysis of ten Utah kindreds and one Texas kindred with multiple cases cutaneous malignant melanoma (CMM) provided evidence that a locus for familial susceptibility is in the chromosomal region 9p13-p22. The genetic markers analyzed reside candidate on chromosome 9p21, previously implicated by presence homozygous deletions tumors germline deletion an individual eight independent melanomas. Multipoint linkage was performed between (MLM) two short tandem repeat markers, D9S126...
TIM-3 is a molecule selectively expressed on subset of murine IFN-gamma-secreting T helper 1 (Th1) cells but not Th2 cells, and regulates Th1 immunity tolerance in vivo. At this time little known about the role human cells. To determine if similarly identifies humans, we generated panel mAb specific for TIM-3. We report that by activated CD4(+) anti-CD3/anti-CD28 stimulation increases both level expression as well number TIM-3(+) also find at high levels vitro polarized lower Th17 In...
Although the physiologic pathways that control regulatory T cells (Foxp3-expressing cells, IL-10-secreting Tr1 cells) and Th17 in rodents have been defined, factors these differentiation humans are not well understood. In this study, we show IL-27 promotes of while inhibiting generation molecules associated with function. Furthermore, inhibits IL-17-polarizing cytokines on dendritic which turn decrease IL-17 secretion from cells. Our results demonstrate plays a key role human by promoting...
T cell immunoglobulin- and mucin domain–containing molecule (TIM)3 is a helper (Th)1–associated surface that regulates Th1 responses promotes tolerance in mice, but its expression function human cells unknown. We generated 104 clones from the cerebrospinal fluid (CSF) of six patients with multiple sclerosis (MS) (n = 72) four control subjects 32) assessed their cytokine profiles levels TIM3 related molecules. MS CSF secreted higher amounts interferon (IFN)-γ than did those subjects,...
A group of women was identified whose risk for breast cancer 47-to 51-fold higher than that experienced by control women. These high-risk were sisters patients mothers had cancer. The disease in these families developed premenopausally, often bilaterally, and seemingly associated with ovarian function. Since susceptibility transmitted from an affected mother to approximately 30% her daughters, this early, bilateral form appeared be strongly heritable. Another, less heritable comprised at...
Multiple sclerosis (MS) is an inflammatory disease of the myelinated central nervous system that postulated to be induced by myelin-reactive CD4 T cells. cell activation requires antigen-specific signal through TCR and a costimulatory signal, which can mediated B7-1 or B7-2 engagement CD28. To directly examine state cells in MS, costimulation requirements necessary activate myelin basic protein (MBP) tetanus toxoid (TT)-reactive were compared between normal controls MS patients. Peripheral...
Glycans on human immunodeficiency virus (HIV) envelope protein play an important role in infection and evasion from host immune responses. To examine the of specific glycans, we introduced single or multiple mutations into potential N-linked glycosylation sites hypervariable regions (V1 to V3) env gene HIV type 1 (HIV-1) 89.6. Three mutants tested showed enhanced sensitivity soluble CD4. Mutant N7 (N197Q) carboxy-terminal stem V2 loop most pronounced increase broadly neutralizing antibodies...
Abstract Multiple sclerosis (MS) is an organ-specific autoimmune disorder that in part genetically determined. The gene encoding the α-chain of IL-2 receptor, IL2RA, harbors alleles associated with risk to MS and other diseases. In addition, IL2RA genetic variants correlate levels a soluble form receptor subjects type 1 diabetes multiple sclerosis. Here, we show genotypes differentially affects IL-2RA (sIL-2RA) cases vs healthy controls; two (rs12722489 rs2104286) account for 15 18% total...
Tumor-associated macrophages (TAMs) contribute substantially to the tumor mass of gliomas and have been shown play a major role in creation microenvironment that promotes progression. Shortcomings attempts at antiglioma immunotherapy may result from failure adequately address these effects. Emerging evidence supports an independent categorization glioma TAMs as alternatively activated M2-type macrophages, contrast classically proinflammatory M1-type macrophages. These exert glioma-supportive...
Abstract Current treatments for bone loss injuries involve autologous and allogenic grafts, metal alloys ceramics. Although these therapies have proved useful, they suffer from inherent challenges, hence, an adequate replacement therapy has not yet been found. We hypothesize that graphene may be a useful nanoscaffold mesenchymal stem cells will promote proliferation differentiation into progenitor cells. In this study, we evaluate graphene, biocompatible inert nanomaterial, its effect on in...
A genetic study of breast cancer is in progress on 500 patients with positive family histories the disease. These were generally younger and had a higher frequency bilaterality than did negative histories. Among familial patients, those early diagnoses (20 to 44 years) manifested significantly rate intermediate (45 54 or late (55 84 years). Blood type O, benign disease, ovarian cysts tumors also tended be more common diagnoses, while blood A, diabetes, obesity, hypertension, uterine...
Objective The purpose of this study was to investigate the timing and mode failure metallic screw-type suture anchors used attach artificial tendons bone in an vivo New Zealand White rabbit model. Study Design Metal with braided composite sutures varying sizes (United States Pharmacopeia (USP) size 1, 2, or 5) were secure replacing both Achilles tibialis cranialis 12 female rabbits. Artificial implanted either at time (immediate replacement, n = 8), four/five weeks after (delayed 4)...
Abstract An investigation of the genetic epidemiology breast cancer involving complex segregation analysis 200 pedigrees Danish extraction is presented. The observed distribution compatible with transmission an autosomal‐dominant gene no evidence for residual family resemblance. frequency abnormal allele 0.00756, and displacement between homozygous genotype means 1.695. accounts a significant proportion in young women, whereas by advanced age majority (87%) affected women are phenocopies....
Abstract Traditionally, emphasis has been placed on the roles of Th cells in generating and amplifying both cellular humoral memory responses. Little is known about potential contributions B cell subsets to immunological memory. Resting have generally regarded as poor APC, attributed part relative paucity costimulatory molecules identified their surface. We describe a novel subpopulation human that express CD80 resting state, are poised secrete particularly large amounts class switched Igs,...
Abstract Multiple sclerosis (MS) is an inflammatory disease of the CNS white matter associated with T cell infiltrates and alterations immune functions that can be measured in peripheral system. TIM-3 has been identified as a central regulator IFN-γ-secreting type 1 Th (Th1) cells tolerance. In this study, using newly generated mAb against human TIM-3, we examined function on ex vivo CD4+ isolated from circulation healthy subjects patients MS. Blocking during stimulation significantly...
The probabilities of developing breast cancer to and between various ages were estimated for 699 sisters patients from three pedigree groups: whose (1) mothers, (2) sisters, or (3) second-degree relatives had previous cancer. source the patient laterality disease important effects on her sister's risk, whereas age at diagnosis virtually no effect. highest life-time risks occurred when bilateral disease, an affected mother (25 ± 7.2%), sister (28 11%). reduced 18 3.3% 14 2.6%, respectively,...
The characteristics of coetaneous melanoma were compared in 106 familial and 2,128 non-familial patients. Familial patients manifested a younger age distribution, significantly earlier average at first diagnosis, increased frequency multiple primary melanomas, higher survival rate than genetic mechanism underlying the type is apparently complex may involve several autonomic gene loci, addition to cytoplasmic component transmitted by an affected or carrier female. nature this component,...