A5006462740- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- BRCA gene mutations in cancer
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Genetic factors in colorectal cancer
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
- Genomic variations and chromosomal abnormalities
- Carcinogens and Genotoxicity Assessment
- Cancer Research and Treatments
- Animal Genetics and Reproduction
- Herpesvirus Infections and Treatments
- Nutrition, Genetics, and Disease
- vaccines and immunoinformatics approaches
- CRISPR and Genetic Engineering
- Ovarian cancer diagnosis and treatment
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 detection and testing
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
University of Wisconsin–Madison
2015-2024
University of Wisconsin Foundation
2022
National Institute of Environmental Health Sciences
1991-2002
National Institutes of Health
1993-2002
Research Triangle Park Foundation
1995-2001
National Cancer Institute
2000
University of North Carolina at Chapel Hill
1997
Triangle
1992-1995
Duke University
1995
Myriad Genetics
1994
A strong candidate for the 17q-linked BRCA1 gene, which influences susceptibility to breast and ovarian cancer, has been identified by positional cloning methods. Probable predisposing mutations have detected in five of eight kindreds presumed segregate alleles. The include an 11-base pair deletion, a 1-base insertion, stop codon, missense substitution, inferred regulatory mutation. gene is expressed numerous tissues, including ovary, encodes predicted protein 1863 amino acids. This contains...
Loss of heterozygosity data from familial tumors suggest that BRCA1 , a gene confers susceptibility to ovarian and early-onset breast cancer, encodes tumor suppressor. The region is also subject allelic loss in sporadic cancers, an indication mutations may occur somatically these tumors. coding was examined for primary show allele at the locus. Mutations were detected 3 32 1 12 carcinomas; all four germline alterations occurred cancers. These results mutation not be critical development...
Linkage analysis of ten Utah kindreds and one Texas kindred with multiple cases cutaneous malignant melanoma (CMM) provided evidence that a locus for familial susceptibility is in the chromosomal region 9p13-p22. The genetic markers analyzed reside candidate on chromosome 9p21, previously implicated by presence homozygous deletions tumors germline deletion an individual eight independent melanomas. Multipoint linkage was performed between (MLM) two short tandem repeat markers, D9S126...
Two amino acids (lysine at position 627 or asparagine 701) in the polymerase subunit PB2 protein are considered critical for adaptation of avian influenza A viruses to mammals. However, recently emerged pandemic H1N1 lack these acids. Here, we report that a basic acid 591 can compensate lysine and confers efficient viral replication Moreover, substantially increased lethality an H5N1 virus mice. We also present X-ray crystallographic structure C-terminus protein. Arginine fills cleft found...
A high-quality rhesus macaque genome Genome technology has improved substantially since the first full organismal genomes were generated. Applying new technology, Warren et al. refined of macaque, a model nonhuman primate. Long-read and other recent advances in sequencing applied to generate with far fewer gaps helped refine locations numbers repetitive elements. Furthermore, authors performed resequencing among populations identify genetic variability macaque. Thus, previously incomplete...
Activated c-Ha-ras protooncogenes have recently been identified in the DNA of some spontaneous hepatic tumors found 2-year-old B6C3 F1 mice. Activation has now demonstrated from well-differentiated hepatomas initiated by a single dose carcinogen given to male mice at 12 days age. each 25 hepatomas, induced N-hydroxy-2-acetylaminofluorene, vinyl carbamate, or 1'-hydroxy-2',3'-dehydroestragole, containing transforming activity NIH 3T3 transfection assay. Southern analysis cells transformed 24...
We have utilized a genetic selection system in yeast to identify novel estrogen-responsive genes within the human genome and define sequences BRCA-1 gene responsible for its estrogen responsiveness. This approach led identification of new subclass Alu family DNA repeats which diverged from known acquired ability function as receptor-dependent enhancers. Importantly, these elements confer responsiveness heterologous promoter when assayed mammalian cells. transcriptional activity can be...
OBJECTIVE To test the graft-promoting effects of mesenchymal stem cells (MSCs) in a cynomolgus monkey model islet/bone marrow transplantation. RESEARCH DESIGN AND METHODS Cynomolgus MSCs were obtained from iliac crest aspirate and characterized through passage 11 for phenotype, gene expression, differentiation potential, karyotype. Allogeneic donor cotransplanted intraportally with islets on postoperative day (POD) 0 intravenously PODs 5 11. Recipients followed stabilization blood glucose...
The p53 gene encodes a nuclear phosphoprotein present in low levels normal human cells. wild-type form of this protein functions to restrain inappropriate cellular proliferation. Approximately one half epithelial ovarian cancers have mutations the and overexpress mutant product. Deletion allele also frequently occurs these cancers.We sought define spectrum cancer with respect both specific codons involved type observed. We examined frequency allelic deletion containing mutations.Tissue...
Pheochromocytomas and paragangliomas have a highly diverse genetic background, with third of the cases carrying germline mutation in 1 14 identified genes.
ABSTRACT Nonhuman primates are widely used to study correlates of protective immunity in AIDS research. Successful cellular immune responses have been difficult identify because heterogeneity within macaque major histocompatibility complex (MHC) genes results quantitative and qualitative differences responses. Here we use microsatellite analysis show that simian immunodeficiency virus (SIV)-susceptible cynomolgus macaques ( Macaca fascicularis ) from the Indian Ocean island Mauritius...
We describe a genome reference of the African green monkey or vervet (Chlorocebus aethiops). This member Old World (OWM) superfamily is uniquely valuable for genetic investigations simian immunodeficiency virus (SIV), which it most abundant natural host species, and wide range health-related phenotypes assessed in Caribbean vervets (C. a. sabaeus), whose numbers have expanded dramatically since Europeans introduced small their ancestors from West Africa during colonial era. use to...
Rhesus macaques ( Macaca mulatta ) are the most widely used nonhuman primate in biomedical research, have largest natural geographic distribution of any primate, and been focus much evolutionary behavioral investigation. Consequently, rhesus one thoroughly studied species. However, little is known about genome-wide genetic variation this A detailed understanding extant genomic among has implications for use species as a model studies human health disease, well population genomics....
Wastewater-based epidemiology (WBE) is an effective way of tracking the appearance and spread SARS-COV-2 lineages through communities. Beginning in early 2021, we implemented a targeted approach to amplify sequence receptor binding domain (RBD) characterize viral present sewersheds. Over course reproducibly detected multiple RBD that have never been observed patient samples 9 sewersheds located 3 states USA. These cryptic contained between 4 24 amino acid substitutions were intermittently...
Abstract Two years after the emergence of SARS-CoV-2, there is still a need for better ways to assess risk transmission in congregate spaces. We deployed active air samplers monitor presence SARS-CoV-2 real-world settings across communities Upper Midwestern states Wisconsin and Minnesota. Over 29 weeks, we collected 527 samples from 15 settings. detected 106 that were positive viral RNA, demonstrating can be continuous variety expanded utility surveillance test 40 other respiratory...
It has been suggested that multiple sites of epithelial ovarian carcinoma on the peritoneal surface reflect polyclonal disease arising from primary tumors in mesothelium, rather than monoclonal spread by metastases one cancer.The purpose this study was to investigate whether cancer a or origin.DNA specimens were obtained peripheral blood lymphocytes (normal DNA) and tumor deposits 17 women with carcinoma: tumors, metastatic deposits, ascites. The clonal origin each determined performing (a)...