- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Microbial Inactivation Methods
- Enzyme Catalysis and Immobilization
- Metal Extraction and Bioleaching
- Mine drainage and remediation techniques
- Fermentation and Sensory Analysis
- Horticultural and Viticultural Research
- Polysaccharides and Plant Cell Walls
- Enzyme-mediated dye degradation
- Plant biochemistry and biosynthesis
- Plant and Biological Electrophysiology Studies
- Radioactive element chemistry and processing
- Mining and Resource Management
Cape Peninsula University of Technology
2018-2023
Nutrasource
2021
A consortium of microbial community was used for the treatment acid mine drainage wastewater laden with sulphate and heavy metals. The treated in an anaerobic continuously stirred tank bioreactor. activity increased pH from 5.6 to 6.5, improved removal up 85% initial concentration 8080 mg SO42−/L a continuous mode, following enrichment 21 d. maximum metal percentage observed Cd (98%), Al (97%), Mn (95%), Pb (94%), Sr (94%) Cu (91%). showed synergy between strictly facultative Firmicutes sp.,...
To sustainably operate a biorefinery with low cost input in commercial setting, the hydrolysis of lignocellulosic biomass must be undertaken manner which will impart environmental tolerance while reducing fermenter inhibitors from delignification process. The challenge lies highly recalcitrant lignin structure, limits conversion holocelluloses to fermentable total sugars (TRS). Due these challenges, sustainable and innovative methods pre-treat developed for delignocellulolytic operations....
In this study, microbial growth kinetics and modeling of alcohols production using Saccharomyces cerevisiae were evaluated different hydrolysates in a single pot (batch) system. Mixed agro-waste from pre-treatment methods, i.e., N. mirabilis/CP HWP/DAP/CP, used as the sole nutrient source fermentations to produce interest. The maximum concentration 1.47 CFU/mL (×1010) was observed with HWP/DAP/CP hydrolysates, relative difference 21.1% when compared cultures; product yield based on biomass...
Human endeavors generate a significant quantity of bio-waste, even lignocellulosic waste, due to rapid industrialization and urbanization, can cause pollution aquatic ecosystems, contribute detrimental animal human health because the toxicity consequent hydrolysis products. This paper contributes new understanding waste bio-pretreatment process from literature review, which provide better biorefinery operational outcomes. The simultaneous partial biological lignin, cellulose hemicellulose...
Grape pomace (GP) generated from the winery industry is one of abundant agro-waste in Western Cape, South Africa and other regions globally. GP contains a significant quantity holocelluloses that can be converted into fermentable total reducing sugars (TRS). This study reports on recovery TRS treated Nepenthes mirabilis digestive fluids for mediated biovalorisation comparison to combination conventional pretreatment methods (hot water pretreatment, dilute acid cellulase pretreatment) single...
This study determined whether it is feasible to pretreat mixed agro-waste of different particle sizes using the pitcher fluid Nepenthes mirabilis (N. mirabilis), which known digest leaf litter due enzyme cocktail contained in fluid. need for holocellulolysis (a source fermentable sugars) produce hydrolysates. The was fractionated (<3 kDa, >3 <10 >10 kDa) and slurrified with agro-waste, i.e., 25% (w/w) each waste—orange peels, apple maize cobs, grape pomace, oak plant various...
In the biofuels industries, agro-waste has been recommended as most promising feedstock for production of bioalcohols.However, challenge utilizing a is due to its highly recalcitrant structure, which limits cellulolytic enzymes convert holocelluloses into fermentable sugars.Different pre-treatments such dilute acid, alkaline, thermal, hot water and enzymatic, have used in previous studies.The with these pre-treatment methods residual toxicants, inhibits high bioalcohol yield, by reducing...
Nepenthes mirabilis pitcher fluid is known to hydrolyse lignocellulosic mixed agro-waste (MAW) into fermentable sugars through a cocktail of oxidative and hydrolytic enzymes. However, the influence factors such as pH, pore size, temperature trace elements on its functionality not adequately understood. This study aims explore potential for pre-treatment MAW (>106 µm) by assessing mentioned above yield total reducible (TRSs). The association between element solution, was evaluated using...