A5042718598- Advanced Fluorescence Microscopy Techniques
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Gene Regulatory Network Analysis
- Bacterial Genetics and Biotechnology
- Supercapacitor Materials and Fabrication
- Single-cell and spatial transcriptomics
- bioluminescence and chemiluminescence research
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- DNA and Nucleic Acid Chemistry
- Photosynthetic Processes and Mechanisms
- Cell Image Analysis Techniques
- Receptor Mechanisms and Signaling
- Advanced biosensing and bioanalysis techniques
- Estrogen and related hormone effects
- Lipid Membrane Structure and Behavior
- Spectroscopy and Chemometric Analyses
- Protein Structure and Dynamics
- Molecular Biology Techniques and Applications
- Advanced Battery Materials and Technologies
- Architecture and Cultural Influences
- Solid-state spectroscopy and crystallography
- Cellular transport and secretion
University College Cork
2023-2025
National University of Ireland
2024
University of Rochester
2024
Boise State University
2014-2023
Texas Tech University Health Sciences Center
2022
Texas Tech University
2022
Toronto Metropolitan University
2021
Inserm
2009-2014
Centre National de la Recherche Scientifique
2009-2014
Université de Montpellier
2009-2014
Synthesis of mRNA in eukaryotes involves the coordinated action many enzymatic processes, including initiation, elongation, splicing, and cleavage. Kinetic competition between these processes has been proposed to determine RNA fate, yet such coupling never observed vivo on single transcripts. In this study, we use dual-color single-molecule imaging living human cells construct a complete kinetic profile transcription splicing β-globin gene. We find that results multiple competing pathways...
Article17 May 2019free access Source DataTransparent process Live-cell imaging reveals the interplay between transcription factors, nucleosomes, and bursting Benjamin T Donovan Biophysics Graduate Program, The Ohio State University, Columbus, OH, USA Search for more papers by this author Anh Huynh Department of Physics, Boise Boise, ID, David A Ball Laboratory Receptor Biology Gene Expression, National Cancer Institute, NIH, Bethesda, MD, Heta P Patel Division Regulation, Netherlands...
Assessing gene expression noise in order to obtain mechanistic insights requires accurate quantification of on many individual cells over a large dynamic range. We used unique method based 2-photon fluorescence fluctuation microscopy measure directly, at the single cell level and with single-molecule sensitivity, absolute concentration fluorescent proteins produced from two Bacillus subtilis promoters that control switch between glycolysis gluconeogenesis. quantified cell-to-cell variations...
Abstract Porosity, composition, and electrode thickness play a vital role in lithium-sulfur (Li-S) batteries. They can be difficult to control make the link between morphology its performance more complex. We evaluated utilization of sulfur during discharge process carbon-sulfur composite with varying porosity, carbon encapsulant type relative mixture ratios, distribution upper lower voltage plateau discharge, overall rate capability electrodes. Chronoamperometry was used as simple fast...
The electrochemical response of symmetric carbon-based supercapacitor devices made using two 3D-printing techniques, Vat-P (vat polymerization) and FDM (fused deposition modeling), shows how the printing method dominates overall cell response. Despite possessing excellent cycle life, conductive poly(lactic acid) (PLA) printed current collectors suffer from relatively high resistance suppressed capacitance linked to collector material resistivity. Here, we examine in situ methods influence...
Kinetoplast DNA, the mitochondrial DNA of trypanosomatid parasites, is a network containing several thousand minicircles and few dozen maxicircles. We compared kinetoplast replication in Trypanosoma brucei Crithidia fasciculata using fluorescence situ hybridization electron microscopy isolated networks. One difference location maxicircles situ. In C. fasciculata, are concentrated discrete foci embedded disk; during increase number but remain scattered throughout disk. contrast, T. generally...
Quantitative characterization of protein interactions in live cells remains one the most important challenges modern biology. In present work we have used two-photon, two-color, fluorescence cross-correlation spectroscopy (FCCS) transiently transfected COS-7 to measure concentrations and estrogen receptor (ER) subtypes alpha beta with their transcriptional coactivator proteins, TIF2, as well heterodimerization between two ER subtypes. Using this approach a systematic fashion, observed strong...
The characterization of RNA–protein complexes (RNPs) is a difficult but increasingly important problem in modern biology. By combining the compact RNA Mango aptamer with fluorogenic thiazole orange desthiobiotin (TO1-Dtb or TO3-Dtb) ligand, we have created an tagging system that simplifies purification and subsequent endogenous RNPs. Mango-tagged RNP can be immobilized on streptavidin solid support recovered their native state by addition free biotin. Furthermore, Mango-based adapted to...
Water-soluble binders are demonstrated to provide significantly better capacity, cycle life stability and rate response for NASICON-type NaTi 2 (PO 4 ) 3 Na-ion battery anodes during reversible sodiation compared electrodes made using polyvinylidene difluoride-containing slurries. The role of carboxymethyl cellulose (CMC) on the physical structure chemical interfacial reactions with sodium-poor uncovered electron microscopy spectroscopy data we show that a more stable NASICON is found from...
A principal component in the protein coats of certain post-golgi and endocytic vesicles is clathrin, which appears as a three-legged heteropolymer (known triskelion) that assembles into polyhedral cages principally made up pentagonal hexagonal faces. In vitro, this assembly depends upon pH, with forming more readily at low pH less high pH. We have developed procedures, on basis static dynamic light scattering, to determine radius gyration, Rg, hydrodynamic radius, RH, isolated triskelia,...
The Central glycolytic genes Repressor (CggR) from Bacillus subtilis belongs to the SorC family of transcription factors that control major carbohydrate metabolic pathways. Recent studies have shown CggR binds as a tetramer its tandem operator DNA sequences and inducer metabolite, fructose 1,6-bisphosphate (FBP), reduces binding cooperativity CggR/DNA complex. Here, we determined effect FBP on size, shape stoichiometry complexes with full-length half-site sequence by small-angle X-ray...
Quantitative spatio-temporal characterization of protein interactions in living cells remains a major challenge facing modern biology. We have investigated neurons the spatial dependence stoichiometry between two core proteins NMDA receptor-associated scaffolding complex, GKAP and DLC2, using novel variation Fluorescence Fluctuation Microscopy called two-photon scanning Number Brightness (sN&B). found that dimerization DLC2 was required for its interaction with GKAP, which turn...
Summary Transcription factors show rapid and reversible binding to chromatin in living cells, transcription occurs sporadic bursts, but how these phenomena are related is unknown. Using a combination of vitro vivo single-molecule imaging approaches, we directly correlated the factor Gal4 with transcriptional bursting kinetics target genes GAL3 GAL10 yeast cells. We find that dwell times sets burst size. time depends on affinity site reduced by orders magnitude nucleosomes. novel platform,...
Gravitational billiards provide a simple method for the illustration of dynamics Hamiltonian systems. Here we examine new billiard system with two parameters, which exhibits, in limiting cases, behaviors previously studied one-parameter systems, namely wedge and parabolic billiard. The consists point mass moving dimensions under influence constant gravitational field hyperbolic lower boundary. An iterative mapping between successive collisions boundary is derived analytically. behavior...
This work describes a single event upset (SEU) experiment performed at the Los Alamos National Laboratory. A closed-loop control system consisting of recoverable flight computer (FCC) and Boeing 737 simulator was operated while FCC exposed to neutron beam. The purpose this test analyze effects bombardment on avionics systems operating altitudes where occurrence is 100 or more times probable than sea level. energy spectrum produced Laboratory similar in shape atmospheric neutrons but much (at...
Metasurfaces offer flexibility for expanding functionality and reducing the size of optical systems by providing from a flat surface. Previous work has demonstrated rapid fabrication testing process wafers containing multiple 1-centimeter diameter metalenses that can be applied towards mass manufacturing. However, quality feedback was limited to analyzing imaging performance parameters such as modulation transfer function focal length. These techniques do not give direct about specific...