A5058324631- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Gene Regulatory Network Analysis
- Bacterial biofilms and quorum sensing
- Microbial Metabolic Engineering and Bioproduction
- Legionella and Acanthamoeba research
- RNA and protein synthesis mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CRISPR and Genetic Engineering
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Evolution and Genetic Dynamics
- Enzyme Structure and Function
- Cell Image Analysis Techniques
- Bacillus and Francisella bacterial research
- Genomics and Phylogenetic Studies
- Microbial Inactivation Methods
- Viral Infectious Diseases and Gene Expression in Insects
- Photosynthetic Processes and Mechanisms
- Slime Mold and Myxomycetes Research
- Vibrio bacteria research studies
- Advanced Fluorescence Microscopy Techniques
- Pharmacological Effects of Natural Compounds
- Fungal Biology and Applications
- Machine Learning in Bioinformatics
Microbiologie de l’alimentation au service de la santé
2016-2025
AgroParisTech
2016-2025
Université Paris-Saclay
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Institut Pasteur
2007-2013
Institut National de la Recherche Agronomique
2012
Centre National de la Recherche Scientifique
2004-2011
Interdisciplinarité en Santé Publique Interventions et Instruments de mesure complexes – Région Est
2011
Laboratoire de Microbiologie et Génétique Moléculaires
2009
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2007
Outside In Acquisition and analysis of large data sets promises to move us toward a greater understanding the mechanisms by which biological systems are dynamically regulated respond external cues. Now, two papers explore responses bacterium changing nutritional conditions (see Perspective Chalancon et al. ). Nicolas (p. 1103 ) measured transcriptional regulation for more than 100 different conditions. Greater amounts antisense RNA were generated expected appeared be produced alternative...
Adaptation of cells to environmental changes requires dynamic interactions between metabolic and regulatory networks, but studies typically address only one or a few layers regulation. For nutritional shifts two preferred carbon sources Bacillus subtilis, we combined statistical model-based data analyses transcript, protein, metabolite abundances promoter activities. malate was rapid primarily controlled posttranscriptionally compared with the slow, mainly transcriptionally adaptation...
Libraries of well-characterised components regulating gene expression levels are essential to many synthetic biology applications. While widely available for the Gram-negative model bacterium Escherichia coli, such libraries lacking Gram-positive Bacillus subtilis, a key organism basic research and biotechnological Here, we engineered genetic toolbox comprising promoters, Ribosome Binding Sites (RBS), protein degradation tags precisely tune in B. subtilis. We first designed modular...
Adaptation to the host environment and exploitation of cell functions are critical success intracellular pathogens. Here, insight these virulence mechanisms was obtained for first time from transcriptional program human pathogen Legionella pneumophila during infection its natural host, Acanthamoeba castellanii. The biphasic life cycle L. reflected by a major shift in gene expression replicative transmissive phase, concerning nearly half genes predicted genome. However, three different...
To transit from intra- to extracellular environments, Legionella pneumophila differentiates a replicative/non-virulent transmissive/virulent form using the two-component system LetA/LetS and global repressor protein CsrA. While investigating how both regulators act co-ordinately we characterized two ncRNAs, RsmY RsmZ, that link CsrA regulatory networks. We demonstrate LetA directly regulates their expression show RsmZ are functional in Escherichia coli able bind vitro. Single mutants have no...
The environmental pathogen Legionella pneumophila encodes three proteins containing F-box domains and additional protein-protein interaction domains, reminiscent of eukaryotic SCF ubiquitin-protein ligases. Here we show that the L. strain Paris are Dot/Icm effectors involved in accumulation ubiquitinated associated with Legionella-containing vacuole. Single, double triple mutants protein encoding genes were impaired infection Acanthamoeba castellanii, THP-1 macrophages human lung epithelial...
Commonly glucose is considered to be the only preferred substrate in Bacillus subtilis whose presence represses utilization of other alternative substrates. Because recent data indicate that malate might an exception, we quantify here carbon source hierarchy. Based on physiology and transcriptional during co-utilization experiments with eight substrates, demonstrate a second for B. subtilis, which rapidly co-utilized strongly uptake From different hierarchy degree catabolite repression...
Legionella pneumophila (Lp) is commonly found in freshwater habitats but also the causative agent of Legionnaires' disease when infecting humans. Although various virulence factors have been reported, little known about nutrition and metabolism bacterium. Here, we report application isotopologue profiling for analyzing L. pneumophila. Cultures Lp were supplied with [U-(13)C(3)]serine, [U-(13)C(6)]glucose, or [1,2-(13)C(2)]glucose. After growth, (13)C enrichments patterns protein-derived...
Abstract Complex regulatory programs control cell adaptation to environmental changes by setting condition‐specific proteomes. In balanced growth, bacterial protein abundances depend on the dilution rate, transcript and transcript‐specific translation efficiencies. We revisited current theory claiming invariance of efficiency. By integrating genome‐wide transcriptome datasets from a library synthetic gfp ‐reporter fusions, we demonstrated that efficiencies in Bacillus subtilis decreased up...
ABSTRACT The yeast Saccharomyces cerevisiae can synthesize trehalose and also use this disaccharide as a carbon source for growth. However, the molecular mechanism by which extracellular be transported to vacuole degraded acid trehalase Ath1p is not clear. By using an adaptation of assay invertase on whole cells with NaF, we showed that more than 90% activity extracellular, splitting into glucose. We found Agt1p-mediated transport hydrolysis cytosolic neutral Nth1p are coupled represent...
In the yeast Saccharomyces cerevisiae, synthesis of endogenous trehalose is catalyzed by a synthase complex, TPS, and its hydrolysis relies on cytosolic/neutral trehalase encoded NTH1. this work, we showed that NTH2, paralog NTH1, encodes functional implicated in mobilization. Yeast also endowed with an acid ATH1 H+/trehalose transporter AGT1, which can together sustain assimilation exogenous trehalose. We tps1 mutant defective TPS catalytic subunit cultivated trehalose, or dual source...
Assessing gene expression noise in order to obtain mechanistic insights requires accurate quantification of on many individual cells over a large dynamic range. We used unique method based 2-photon fluorescence fluctuation microscopy measure directly, at the single cell level and with single-molecule sensitivity, absolute concentration fluorescent proteins produced from two Bacillus subtilis promoters that control switch between glycolysis gluconeogenesis. quantified cell-to-cell variations...
Abstract Genome-scale engineering enables rational removal of dispensable genes in chassis genomes. Deviating from this approach, we applied greedy accumulation deletions large regions the Bacillus subtilis genome, yielding a library 298 strains with genomes reduced up to 1.48 Mb size. High-throughput physiological phenotyping these confirmed that genome reduction is associated substantial loss cell fitness and synthetic-sick interactions. Transcriptome analysis indicated <15%...
Plasmid pBaSysBioII was constructed for high-throughput analysis of gene expression in Bacillus subtilis . It is an integrative plasmid with a ligation-independent cloning (LIC) site, allowing the generation transcriptional gfpmut3 fusions desired promoters. Integration by Campbell-type event and non-mutagenic, placing fusion at homologous chromosomal locus. Using phoA , murAA gapB, ptsG cggR promoters that are responsive to phosphate availability, growth rate carbon source, we show detailed...
Most organisms can choose their preferred carbon source from a mixture of nutrients. This process is called catabolite repression. The Gram-positive bacterium Bacillus subtilis uses glucose as the and energy. Glucose-mediated repression caused by binding CcpA transcription factor to promoter regions catabolic operons. binds DNA upon interaction with its cofactors HPr(Ser-P) Crh(Ser-P). formation catalyzed metabolite-activated HPr kinase/phosphorylase. Recently, it has been shown that malate...
At the heart of central carbon metabolism, pyruvate is a pivotal metabolite in all living cells. Bacillus subtilis able to excrete as well use it sole source. We herein reveal that ysbAB (renamed pftAB), only operon specifically induced pyruvate-grown B. cells, encodes hetero-oligomeric membrane complex which operates facilitated transport system specific for pyruvate, thereby defining novel class transporter. demonstrate LytST two-component responsible induction pftAB presence by binding...
Abstract Probiotics are widely used for their health promoting effects, though a lot remain to be discovered, particularly on mechanisms of action at the molecular level. The functional genomic approach is an appropriate method decipher how probiotics may influence human cell fate and therefore contribute benefit. In present work, we focused Shouchella clausii (formerly named Bacillus then Alkalihalobacillus ), spore-forming bacterium that commercially available as probiotic prevention...
Abstract Polymerase errors during DNA replication are a major source of point mutations in genomes. The spontaneous mutation rate also depends on the counteracting activity repair mechanisms, with mutator phenotypes appearing constantly and allowing for periods rapid evolution nature laboratory. Here, we use Gram-positive model bacterium Bacillus subtilis to disentangle contributions polymerase initial nucleotide selectivity, proofreading, mismatch (MMR) rate. To achieve this, constructed...
We report that autonomous oscillations, which usually happen in aerobic glucose‐limited continuous cultures of yeast at low dilution rate, were also observed trehalose discontinuous Saccharomyces cerevisiae . This unexpected oscillatory behaviour was therefore examined using fast Fourier transformation online gas measurements. robust mathematical analysis underlined the existence two types oscillation. The first found to be linked cell cycle because (a) periodicity corresponded a fraction...