A5100410842- Advanced biosensing and bioanalysis techniques
- Retinoids in leukemia and cellular processes
- Circular RNAs in diseases
- DNA and Nucleic Acid Chemistry
- Enzyme Catalysis and Immobilization
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Electrochemical sensors and biosensors
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Cerebrovascular and genetic disorders
- Metalloenzymes and iron-sulfur proteins
- Acute Myeloid Leukemia Research
- Biosensors and Analytical Detection
- Photosynthetic Processes and Mechanisms
- Moyamoya disease diagnosis and treatment
- Natural product bioactivities and synthesis
- Inflammasome and immune disorders
- Advanced Biosensing Techniques and Applications
- Click Chemistry and Applications
- Cell death mechanisms and regulation
- NF-κB Signaling Pathways
- Advanced Differential Equations and Dynamical Systems
- Biochemical and Molecular Research
- Autophagy in Disease and Therapy
Southern Medical University
2025
Nanfang Hospital
2025
Tianjin University of Traditional Chinese Medicine
2023-2024
Huazhong University of Science and Technology
2006-2024
Tongji Hospital
2021-2024
Central Hospital of Wuhan
2024
Fuzhou University
2023
Jiujiang University
2012-2023
Hubei Provincial Hospital of Traditional Chinese Medicine
2023
Harbin University of Commerce
2023
Here we have developed a sensitive DNA amplified detection method based on isothermal strand-displacement polymerization reaction. This takes advantage of both the hybridization property and polymerase. Importantly, demonstrate that our produces circular reaction activated by target, which essentially allows it to self-detect. Functionally, this system consists hairpin fluorescence probe, short primer Upon recognition with target ssDNA, stem probe is opened, after opened anneals triggers...
Abstract Circular RNAs (circRNAs) have emerged as novel molecules of interest in gene regulation other noncoding RNAs. Though they been explored some species and tissues, the expression functions circRNAs human reproductive systems remain unknown. Here we revealed profiles testis tissue using high-throughput sequencing. The conformation these testis-derived seminal plasma was also investigated, aiming to provide a non-invasive liquid biopsy surrogate for testicular biopsy. We predicted...
A DNA configuration switch is designed to fabricate a reversible and regenerable Raman-active substrate. The substrate composed of Au film hairpin-shaped strand (hot-spot-generation probes, HSGPs) labeled with dye-functionalized silver nanoparticles (AgNPs). Another ssDNA that recognizes specific trigger used as an antenna. HSGPs are immobilized on the draw AgNPs close surface create intense electromagnetic field. Hybridization HSGP two arm segments antenna forms triplex-stem structure...
Circular RNAs (circRNAs) have recently been shown to exert effects on multiple pathological processes by acting as miRNA sponges. However, the roles of circRNAs in ovarian senescence are largely unknown. The objective this study was identify involved aging and predict their potential biological functions. We first performed RNA-sequencing generate circRNA expression profiles from young (n = 3) groups. In total, 48,220 were identified, which 194 significantly up-regulated 207 down-regulated...
Abstract BACKGROUND To develop an environment‐friendly approach for the synthesis of (S)‐1‐(2,6‐dichloro‐3‐fluorophenyl) ethyl alcohol, intermediate anti‐cancer drug Crizotinib, aldehyde ketone reductase and alcohol dehydrogenase were overexpressed in Escherichia coli Rosetta (DE3) purified via chromatography. Subsequently, they co‐crystalized with CaHPO 4 at 4°C to form dual‐enzyme@CaHPO hybrid nanoflowers (hNFs) which was then used catalyze along evaluation its thermal stability recycling...
To develop a method for preparing rapidly, precisely, and bio-orthogonally cross-linked enzymes (RP-CLEs), nonstandard amino acids (NSAAs) were inserted into the enzyme protein, microwave irradiation was used to accelerate its site-specific linkage through Cu-free strain-promoted alkyne–azide cycloaddition (SPAAC). this end, we selected aldehyde ketone reductase (AKR) as model enzyme, AKR mutants obtained by five-point insertion of p-azido-l-phenylalanine (pAzF) which subsequently form...
Alcoholic liver disease (ALD) is associated with gut dysbiosis and hepatic inflammasome activation. While it known that antimicrobial peptides (AMPs) play a critical role in the regulation of bacterial homeostasis ALD, functional AMPs alcohol-induced activation unclear. The aim this study was to determine effects cathelicidin-related peptide (CRAMP) on ALD. CRAMP knockout (Camp-/-) wild-type (WT) mice were subjected binge-on-chronic alcohol feeding synthetic administered. Serum/plasma tissue...
Chloroplast development and photosynthesis require the proper assembly turnover of photosynthetic protein complexes. Chloroplasts harbor a repertoire proteases to facilitate proteostasis development. We have previously used an Arabidopsis leaf variegation mutant, yellow variegated2 (var2), defective in thylakoid FtsH protease complexes, as tool dissect genetic regulation chloroplast Here, we report new enhancer mutant var2, variegation3–1 (evr3–1). confirm that EVR3 encodes metalloprotease,...
DNA (cytosine-5)-methyltransferase 3A (DNMT3A)-mutated acute myeloid leukemia (AML) has a poor prognosis, but the exact mechanism is still unclear. Here, we aimed to explore of immune escape in AML with DNMT3A mutation. We constructed knockout clone and DNMT3A-R882H-mutated clones. RNA-seq results showed that transcription factors macrophage inflammatory proteins were significantly downregulated mutant KEGG enrichment gene set analysis (GSEA) large number genes enriched immune-related...