Spermatogonial stem cell (SSC) transplantation has been shown to restore fertility in several species and may have application for treating some cases of male infertility (e.g., secondary gonadotoxic therapy cancer). To ensure safety this preservation strategy, methods are needed isolate enrich SSCs from human testis suspensions also remove malignant contamination. We used flow cytometry characterize surface antigen expression on testicular cells leukemic (MOLT-4 TF-1a). demonstrated via...

Uterine leiomyomas are benign tumors that can cause pain, bleeding, and infertility in some women. Mediator complex subunit 12 (MED12) exon 2 variants associated with uterine leiomyomas; however, the causality of MED12 variants, their genetic mode action, role genomic instability have not been established. Here, we generated a mouse model conditionally expresses Med12 missense variant (c.131G>A) uterus demonstrated this alteration alone promotes leiomyoma formation hyperplasia both WT mice...

A commercial red tilapia farm was diagnosed with Streptococcus agalactiae infection using histopathology, microbiology and immunohistochemistry. One hundred fish were randomly taken from different weight/age groups including broodstock, market (larger than 150 g), on-growing between 20 g, juveniles larvae. Fish clinically examined, anaesthetised necropsied. Samples brain, liver, spleen, eyes kidney for microbiology. All organs processed histopathology an indirect immunoperoxidase test (IIP)....

Abstract Streptococcus agalactiae causes a severe systemic disease in fish, and the routes of entry are still ill‐defined. To address this issue, two groups 33 red tilapia Oreochromis spp. each 10 g were orally infected with S . ( n = 30), by immersion six individuals control‐uninfected fish. Three tilapias killed at time point from 30 min to 96 h post‐inoculation pi ); controls h. Samples most tissues examined haematoxylin–eosin (H&E), indirect immunoperoxidase IPI ) periodic...

The distribution of antigen and pathological changes induced by an experimental infection with Aeromonas hydrophila strain KJ99, its extracellular products, were studied in two species fish. microorganism was disseminated systemically the haemodynamic tissue similar to those observed septicaemia mammals. Intussusception, degeneration necrosis nervous plexus muscular layers gastrointestinal tract common findings.

The histological lesions caused by Edwardsiella tarda in a variety of fish species, including tilapia, have been well characterized. There are apparent differences the type inflammatory response manifested these different which may be due to species itself, phase infection, or virulence factors produced strains E. tarda. In catfish, systemic abscesses involving muscles flank caudal peduncle most common lesions. By contrast, infection tilapia and red sea bream is more likely associated with...

Abstract Ciprofloxacin (CFX) implants containing poly( D,L ‐lactide) and calcium phosphates (tricalcium phosphate hydroxyapatite) was evaluated in 50 rabbits an experimental osteomyelitis model. Their femoral cavity inoculated with Staphylococcus aureus . After 2 weeks, the infected focus cleaned out delivery system implanted. The infection subsequent response to treatment were by microbiological analysis, biochemical hematological markers, body weight, temperature, clinical signs, X‐rays,...

Rickettsial organisms are well-known fish pathogens in both natural and culture environments. This study reports an outbreak of disease red tilapia larvae caused by piscirickettsia-like (PLOs), which lasted from June until October 2009. Severe mortality was recorded almost exclusively postlarvae aged 1–22 days old. Although clinical or gross findings were not evident diseased fish, histopathology revealed severe necrosis the epidermis gill epithelium, with concomitant changes underlying...

Multidrug resistant Acinetobacter baumannii (MDR AB) with and without Staphylococcus aureus (SA) is a commonly isolated organism in infected segmental bone defects combat-related trauma Iraq Afghanistan. Although MDR AB visceral infections therapeutic challenge, control of infection appears more common for osteomyelitis.Using rat model, we explored the virulence alone combination SA.Segmental 60 femurs were created, stabilized, inoculated SA. We performed qualitative quantitative...

To investigate the effect of systemic administration immunosuppressant dexamethasone (DM) while inducing hamster buccal pouch DMBA carcinogenesis. Materials and Methods. Two different experiments were performed. In first experiment, hamsters' right pouches in group A (n = 10) painted three times per week with 7,12-dimethylbenzanthracene (DMBA) 0.5%, animals B (.The time macroscopic neoplasm development was reduced when DM-DMBA coexposition employed, finding tumors after 10-12 weeks...

This study aimed to evaluate the ability of a panel lectins inhibit Pasteurella multocida adhere and affect rabbit respiratory epithelium. Nasal septa from fetuses were cultured with various before addition P. multocida. The percentage bacteria adhering epithelium was evaluated semiquantitatively by indirect immunoperoxidase (IIP) staining. goblet cells (GCs) counted in semithin sections stained toluidine blue served as main morphological criterion inhibitory effect lectins. PNA, WGA,...

The nasal septa of fetal rabbits at 26 days gestation were harvested by cesarean section the does while under anesthesia and then exposed to Bordetella bronchiseptica or its lipopolysaccharide (LPS) for periods 2 4 hours. A total 240 explants used. tissues examined using Hematoxylin &amp; Eosin technique. Then, semithin sections (0.5 <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>μ</mml:mi></mml:mrow></mml:math>m) stained with toluidine blue indirect...

Abstract The use of histopathology in fish sciences is broadly extended, although it currently devoid standardization across the literature. There have been initiatives to standardize every step histological evaluation, including description, diagnosis, interpretation, data recording and reporting, statistical analysis, but, general, histopathological systems applied date present a series limitations that hamper reproducibility derived data. On top these limitations, an agreed,...

El lipopolisacárido (LPS) o endotoxina es el mayor componente de la membrana externa las bacterias Gram negativas, desempeñan una importante función en activación del sistema inmune al constituir antígeno superficial más este tipo bacterias. LPS está compuesto por región lípidica y glicosídica con funciones separadas y/o sinérgicas lo que hace esta molécula uno los factores virulencia complejos comprender, revisión pretende hacer un acercamiento para dimensionar universalidad diversidad...

The role of the P. multocida lipopolysaccharide (LPS) as a putative adhesin during early stages infection with this bacterium in respiratory epithelium rabbits was investigated. By light microscopy and double enzyme labeling nasal septa tissues, amount bacteria attached to LPS present goblet cells at different experimental times were estimated. Transmission electron (TEM) colloidal gold particles also used determine exact location cells. Septa that challenged 30 minutes later showed more...