Fat-structured food materials are an important component of our diet. The role that fat plays in material functionality, flavor perception, texture and health characteristics is due large part to its physical properties. An understanding these properties relevant from scientific, technological medical perspectives. materials, are, turn, governed by a complex confluence the various structural levels beginning with triglyceride molecules. formation nanoscale elements molecules first step as we...

ABSTRACT Atomic force microscopy was used to measure the thickness of air-dried, collapsed murein sacculi from Escherichia coli K-12 and Pseudomonas aeruginosa PAO1. Air-dried E. had a 3.0 nm, whereas those P. were 1.5 nm thick. When rehydrated, both bacteria swelled double their anhydrous thickness. Computer simulation section model single-layer peptidoglycan network in an aqueous solution with Debye shielding length 0.3 gave mass distribution full width at half height 2.4 essential...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRaman scattering in bilayers of saturated phosphatidylcholines. Experiment and theoryDavid A. Pink, Trevor J. Green, Dennis ChapmanCite this: Biochemistry 1980, 19, 2, 349–356Publication Date (Print):January 22, 1980Publication History Published online1 May 2002Published inissue 22 January 1980https://pubs.acs.org/doi/10.1021/bi00543a016https://doi.org/10.1021/bi00543a016research-articleACS PublicationsRequest reuse permissionsArticle...

Nanoscale materials can have cytotoxic effects. Here we present the first combined empirical and theoretical investigation of influence electrostatic attraction on nanoparticle cytotoxicity. Modeling interactions between cells 13 nm spheres zinc oxide nanoparticles provided insight into empirically determined variations minimum inhibitory concentrations four differently charged isogenic strains Pseudomonas aeruginosa PAO1. We conclude that controlling their cellular targets may permit modulation

Simple models are developed for the first-order (main) gel–fluid phase transition in lipid mono-and bilayers. The chains assumed to lie on a triangular lattice both phases and interact via anisotropic van der Waals forces. article begins with review of previous experimental theoretical work. Next two-state Ising-like model is shown give good qualitative description main mono- bilayers statistical fluctuations about this transition. continues more quantitative 10-state bilayer due Pink. polar...

A model of the outer membrane Gram-negative bacteria was created by deposition a monolayer purified rough mutant lipopolysaccharides at an air/water interface. The density profiles monovalent (K + ) and divalent (Ca 2+ cations normal to (LPS) monolayers were investigated using grazing-incidence X-ray fluorescence. In absence Ca , K concentration peak found in negatively charged LPS headgroup region. With addition CaCl 2 ions almost completely displaced from By integrating experimentally...

To investigate the use of confocal Raman microspectroscopy (CRM) for analysis structure, composition and development fully hydrated biofilms.Pseudomonas aeruginosa PAO1 biofilms were cultured in a flow cell minimal nutrient medium (artificial sea water) their was followed up to 3 weeks. The spectroscopic signature biofilm cells extracellular polymeric substances (EPS) differentiated distribution colonies within water channels mapped in-plane -depth. initially amorphous, mainly composed with...

The protein precipitating capacity of canola tannins was evaluated using the precipitation assay Hagerman and Butler (J. Agric. Food Chem. 1978, 26, 809−812) dye-labeled bovine serum albumin (BSA) Asquith Ecol. 1985, 11, 1535−1543). Condensed were isolated from hulls Cyclone, Excel, Westar cultivars. tannin content in ranged 98 to 1973 mg catechin equivalents/100 g hulls, as determined by vanillin assay. effect pH on affinities unlabeled BSA, fetuin, gelatin, lysozyme, pepsin monitored....

Monte Carlo techniques have been applied to a study of two related quasi-two-dimensional microscopic interaction models which describe the phase behavior phospholipid bilayers. The are Ising-like lattice in (a) acyl chains phospholipids interact via anisotropic van der Waals forces and (b) rotational isomerism is accounted for by ten selected conformational states, respectively. experiments performed on both so as determine whether static thermodynamic properties lipid bilayers most...

A lattice model has been developed to study the effects of intrinsic membrane proteins upon thermodynamic properties a lipid bilayer membrane. We assume that only nearest-neighbor van der Waals and steric interactions are important polar group can be represented by effective pressure-area terms. Phase diagrams, temperature T(0), which locates gel-fluid melting, transition enthalpy, correlations were calculated mean field cluster approximations. Average chain areas when is in given protein...

Ultra-small angle X-ray scattering has been used for the first time to elucidate, in situ, aggregation structure of a model edible oil system. The three-dimensional nano- micro-structure tristearin solid particles triolein solvent was investigated using 5, 10, 15, and 20% solids. Three different sample preparation procedures were investigated: two slow cooling rates 0.5°/min, case 1 (22 days storage at room temperature) 2 (no storage), one fast 30°/min, 3 storage). length scale investigated,...

Triacylglycerols (TAGs) are biologically important molecules which form the recently discovered highly anisotropic crystalline nanoplatelets (CNPs) and, ultimately, large-scale fat crystal networks in edible oils. Identifying hierarchies of these and how they spontaneously self-assemble is to understanding their functionality oil binding capacity. We have modelled CNPs studied aggregate under assumption that all present before aggregation begins solubility liquid very low. represented as...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCondensed Tannins in Canola HullsMarian Naczk, Tom Nichols, David Pink, and Frank SosulskiCite this: J. Agric. Food Chem. 1994, 42, 10, 2196–2200Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October 1994https://pubs.acs.org/doi/10.1021/jf00046a022https://doi.org/10.1021/jf00046a022research-articleACS PublicationsRequest reuse permissionsArticle Views479Altmetric-Citations48LEARN ABOUT...

Lipopolysaccharide (LPS) monolayers deposited on planar, hydrophobic substrates were used as a defined model of outer membranes Pseudomonas aeruginosa strain dps 89. To investigate the influence ions (out-of-plane) monolayer structure, we measured specular X-ray reflectivity at high energy (22 keV) to ensure transmission through water. Electron density profiles reconstructed from curves, and they indicate that presence Ca(2+) induces significant change in conformation charged polysaccharide...

Ultra-small angle x-ray scattering (USAXS) has been used to elucidate, in situ, the aggregation structure of unsheared model edible oils. Each system comprised one or two solid lipids and a combination liquid lipids. The 3D nano- micro-structures each were characterized. length scale investigated, using Bonse-Hart camera at beamline ID–15D Advanced Photon Source, ANL, ranged from 300 Å–10 µm. Using Unified Fit model, level-1 analysis showed that scatterers 2D objects with either smooth,...

The liquid–solid phase transition of triacylglycerols (TAGs), the main components edible fats and oils is central to production sensory properties many processed foods. While there has been extensive research on nucleation growth fats, remains a dearth knowledge regarding structural organization TAGs in liquid state. From molecular perspective, consist three alkyl chains esterified glycerol backbone. Several models based experiment simulation have helped unveil TAG molten However, more...

Ellman's procedure has been used to study the oxidation rates of cysteine (CSH) and glutathione (GSH) in aqueous solutions, it was reported that, for CSH, number sulfhydryl molecules not oxidized became zero at a specific time, called tc, where be finite. We point out that under very general considerations, we should observe tc unbounded, becomes infinite. The reason is as process forming disulfide bond proceeds, probability two CSH finding each other eventually vanishingly small so...

The contents of soluble, SDS-extractable, and insoluble condensed tannins were determined in canola/rapeseed hulls from several varieties by utilizing the proanthocyanidin assay. total amount rapeseed/canola ranged 1913 to 6213 mg per 100 g oil-free hulls. Insoluble predominated comprised 70 95.8% present. amounts SDS-extractable comparable those soluble but constituted only 4.7−14.1% Keywords: tannins; hulls; assay; canola; rapeseed

Fluorescence probe data using 1,6-diphenyl-1,3,5-hexatriene for various concentrations of intrinsic molecules (cholesterol, gramicidin A and cytochrome oxidase) within fluid lipid bilayers have been examined. The polarization value increases with increasing concentration molecule then approaches a limiting value. Empirical curve-fitting the experimental data, change concentration, shows that each system can be fitted approximately by an exponential curve. theory has constructed based upon...

Long-range electrostatic interactions are generally assigned a subordinate role in the high-affinity binding of proteins by glycosaminoglycans, most highly charged biopolyelectrolytes. The discovery high and low sulfation domains heparan sulfates, however, suggests selectivity via complementarity their linear patterns with protein charge patterns. We examined how sequences anionic/nonionic copolymers affect to prominent anisotropy. Experiments united-atom Monte Carlo simulations, together...

Protamine is a cationic antimicrobial peptide, which inhibits or kills number of Gram-negative bacteria, including Pseudomonas aeruginosa and Escherichia coli. Electrostatic interactions between the outer leaflet membrane protamine are thought to be important for effect. We hypothesized that divalent ions would compete with binding charged O-sidechain liposaccharide expel from O-sidechains. Experimentally it was shown increasing concentrations cations (Ca2+ Mg2+) reduced effect on P. PA01 E....