A5060916224- Reproductive Biology and Fertility
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Sperm and Testicular Function
- RNA Research and Splicing
- Effects and risks of endocrine disrupting chemicals
- RNA and protein synthesis mechanisms
- COVID-19 Impact on Reproduction
- Advanced biosensing and bioanalysis techniques
- Renal and related cancers
- Health, Environment, Cognitive Aging
- Reproductive System and Pregnancy
- RNA Interference and Gene Delivery
- Autism Spectrum Disorder Research
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Parvovirus B19 Infection Studies
- Genetics, Aging, and Longevity in Model Organisms
- Telomeres, Telomerase, and Senescence
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- DNA and Nucleic Acid Chemistry
- Endometriosis Research and Treatment
Sheba Medical Center
2017-2025
Tel Aviv University
2020-2025
Bar-Ilan University
2008-2014
Qatar University
2011
Abstract Objective No information exists in the literature regarding effect of mRNA SARS-CoV-2 vaccine on subsequent IVF cycle attempt. We therefore aim to assess influence treatments. Design An observational study. Setting A tertiary, university-affiliated medical center. Patients and Methods All couples undergoing consecutive ovarian stimulation cycles for before after receiving vaccine, reached ovum pick-up (OPU) stage. The characteristics embryological variables treatments were assessed...
Exported mRNAs are targeted for translation or can undergo degradation by several decay mechanisms. The 5'-->3' machinery localizes to cytoplasmic P bodies (PBs). We followed the dynamic properties of PBs in vivo and investigated mechanism which scan cytoplasm. Using proteins decapping machinery, we asked whether actively cytoplasm a diffusion-based is sufficient. Live-cell imaging showed that were anchored mainly microtubules. Quantitative single-particle tracking demonstrated most...
The 5′→3′ mRNA degradation machinery localizes to cytoplasmic P bodies (PBs), which are non-membranous structures found in all eukaryotes. While PB function has been intensively studied yeast, less is known mammalian cells, such as whether enzymes actively engaged or if PBs serve storage depots, particularly during cellular stress. We examined the fate of mRNAs translational stress, and show that accumulate within amino acid starvation. 5′ 3′ ends transcripts residing could be identified,...
Objective No information exists in the literature regarding effect of coronavirus disease 19 (COVID-19) infection on subsequent vitro fertilization (IVF) cycle attempt. We, therefore, aim to assess influence COVID-19 IVF treatments.
Abstract Human embryogenesis frequently coinciding with cell division mistakes contributing to pervasive embryonic aneuploidy/mosaicism. While embryo self-correction was elegantly demonstrated in mouse models, human studies are lacking. Here we witness embryos ability eliminate/expel abnormal blastomeres as debris/fragments. Each blastocyst and its corresponding debris were separated underwent whole genome amplification. Seven of the 11 pairs blastocysts their debris/fragments revealed...
Indicators of male fertility are in decline globally, but the underlying causes, including role environmental exposures, unclear. This study aimed to examine organic chemical pollutants seminal plasma, both known priority chemicals and less studied chemicals, identify uncharacterized reproductive toxicants. Semen samples were collected from 100 individuals assessed for sperm concentration, percent motility, total motile sperm. Targeted nontargeted pollutant exposures measured plasma using...
Processing bodies (PBs) are non-membranous cytoplasmic structures found in all eukaryotes. Many of their components such as the Dcp1 and Dcp2 proteins highly conserved. Using live-cell imaging we that PB disassembled cells prepared for cell division, then began to reassemble during late stages cytokinesis. During cycle passed through S phase, numbers increased. However, there was no memory between mother daughter cells. Examination hDcp1a hDcp1b by electrophoresis mitotic extracts showed a...
The research and development of a matrix Addressable Nanoliter Containers (ANLCs) is the focus this work. ANLCs introduce novel approach for cryopreserving single sperm cells. A significant increase in cell mortality was observed after nanoliter-scale suspensions, attributed to diffusion water from aqueous droplets into surrounding oil phase. This process elevated salt concentration within droplets. practical solution devised by saturating with water, significantly reducing gradient and,...
In recent years, the application of blastocyst biopsy in PGT has been gradually rising, mainly due to assumed detrimental effect blastomere on embryo implantation potential and widespread for aneuploidy. contrast complete chromosomal testing (CCT) cycles, which trophectoderm (TE) become well-established preferred method higher diagnostic reliability, evidences purpose PGT-M are still lacking. Therefore, we conducted a retrospective cohort study including 147 cycles with at least eight high...
Abstract Background Intracytoplasmic sperm injection (ICSI) is an effective technique for addressing male infertility. However, fertilization challenges persist, particularly with spermatozoa obtained through testicular procedures. Objectives This study evaluates the impact of artificial oocyte activation (AOA) on ICSI outcomes using surgically retrieved (motile, immotile, fresh, and frozen), including results from vitrified‐warmed embryo transfers investigating potential improvements in...
Abstract Biological macromolecules, including DNA, RNA, and proteins, have intrinsic features that make them potential building blocks for the bottom‐up fabrication of nanodevices. Unlike RNA is a more versatile molecule whose range in cell from 21 to thousands nucleotides usually folded into stem loop structures. unique nanoscale due its diversity size, function, structure. Because gene expression analysis becoming clinical reality there need collect minute amounts samples, keeping intact...
Ultrasonic waves can be used to convert native DNA molecules into nanospheres. This sonochemical nanospherization could as a method of protecting from degradation in harsh environments. Due its carrier genetic information, spheres biocompatible material for the delivery information cells. Detailed facts importance specialist readers are published "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note: The...
Abstract Human embryos utilise an array of processes to eliminate the very high prevalence aneuploid cells in early embryo stages. self-correction was recently demonstrated by their ability eliminate/expel abnormal blastomeres as cell debris/fragments. A whole genome amplification study has that 63.6% blastocysts expelled debris with chromosomal rearrangements. Moreover, 55.5% euploid expel debris, strongly suggesting primary source free DNA culture media is and/or fragments. Such a...
Fragile X premutation (Amplification of CGG number 55-200) is associated with increased risk for fragile X-Associated Premature Ovarian Insufficiency (FXPOI) in females and X-associated tremor/ataxia syndrome (FXTAS) predominantly males. Recently, it has been shown that repeats trigger repeat non-AUG initiated translation (RAN) a cryptic polyglycine-containing protein, FMRpolyG. This protein accumulates ubiquitin-positive inclusions neuronal brain cells FXTAS patients may lead to...
OBJECTIVE: To investigate whether the addition of embryo biopsy performed during preimplantation genetic testing for monogenic diseases is associated with a higher risk obstetric and neonatal complications compared in vitro fertilization (IVF) without or spontaneously conceived pregnancies. METHODS: This cohort study all pregnancies after (PGT-M group) from 2006 to 2018 at Sheba Medical Center, Israel. The control groups included patients who had (spontaneous conception by IVF (IVF delivered...
Summary Embryo transfer is a crucial step in IVF cycle, with increasing trend during the last decade of transferring single embryo, preferably at blastocyst stage. Despite evidence supporting Day 5 blastocyst-stage transfer, optimal day embryo remains controversial. The questions are therefore, whether mechanisms responsible to embryos arrest aneuploidy or others, and those arrested in-vitro between cleavage stage would survive in-vivo if transferred on cleavage-stage. We therefore aim...