A5050763033- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Insect Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Pineapple and bromelain studies
- Insect and Pesticide Research
- Nematode management and characterization studies
- Fungal Plant Pathogen Control
- Banana Cultivation and Research
- Metalloenzymes and iron-sulfur proteins
- Plant Molecular Biology Research
- HIV Research and Treatment
- Hepatitis B Virus Studies
- Plant pathogens and resistance mechanisms
- Plant Stress Responses and Tolerance
- Hepatitis C virus research
Purdue University West Lafayette
1995-2002
Scripps Research Institute
1994
The Pti4, Pti5, and Pti6 proteins from tomato were identified based on their interaction with the product of Pto disease resistance gene, a Ser-Thr protein kinase. They belong to ethylene-response factor (ERF) family plant-unique transcription factors bind specifically GCC-box cis element present in promoters many pathogenesis-related (PR) genes. Here, we show that these ERFs are localized nucleus function vivo as activators regulate expression GCC box–containing PR Expression or Arabidopsis...
Abstract The octadecanoid signaling pathway has been shown to play an important role in plant defense against various chewing insects and some pathogenic fungi. Here, we examined the interaction of a cell-content feeding arachnid herbivore, two-spotted spider mite (Tetranychus urticae Koch), with cultivated tomato (Lycopersicon esculentum) isogenic mutant line (defenseless-1 [def-1]) that is deficient biosynthesis pathway-derived signal, jasmonic acid (JA). Spider fecundity ondef-1 plants...
Transgenic mice that express the hepatitis B virus core protein were used to examine factors influence intracellular localization of nucleocapsid particles in primary hepatocyte vivo. In this model, viral are strictly localized nucleus except when nuclear membrane dissolves during cell division, at which time they enter cytoplasm. The cytoplasmic do not reenter nucleus, however, re-forms after division. data support notion can form de novo within and suggest performed cannot be transported...
The catalytic activity and amino acid specificity of the tomato Pto Fen kinases were investigated. genes fused to carboxyl terminus maltose-binding protein expressed in Escherichia coli. Incubation purified fusion proteins with [gamma-32P]ATP an vitro assay showed that both capable autophosphorylation. Mutant which conserved lysine residue subdomain II was changed a glutamine unable autophosphorylate. Phosphoamino analysis active indicated phosphorylate serine threonine residues but not tyrosine.
The Pto gene was derived originally from the wild tomato species Lycopersicon pimpinellifolium and confers resistance to Pseudomonas syringae pv strains expressing avirulence avrPto. Fen is also L. sensitivity insecticide fenthion. We have now isolated characterized alleles of cultivated tomato, esculentum, designated them pto fen. High conservation genome organization between two allowed us identify fen among cluster closely related family members. are transcribed uninterrupted open reading...
Resistance to bacterial speck in tomato is governed by a gene-for-gene interaction which single resistance locus (Pto) the plant responds expression of specific avirulence gene (avrPto) pathogen. Disease susceptibility results if either Pto or avrPto are lacking from corresponding organisms. Leaves cultivars that contain also exhibit hypersensitive-like response upon exposure an organophosphorous insecticide, fenthion. Recently, was isolated map-based cloning approach and shown be member...
In tomato plants, resistance to bacterial speck disease is mediated by a phosphorylation cascade, which triggered the specific recognition between plant serine/threonine protein kinase Pto and AvrPto protein. present study, we investigated in vitro biochemical properties of Pto, appears function as an intracellular receptor for signal molecule. its downstream effector Pti1, also kinase, were expressed Escherichia coli maltose-binding glutathioneS-transferase fusion proteins, respectively....
The tomato Pto kinase confers resistance to bacterial speck disease caused by strains of Pseudomonas syringae pv. that express the avirulence gene avrPto. contains a putative myristylation site at its amino terminus was hypothesized play role in localizing plant cell. Site-directed mutagenesis used change invariant glycine residue motif an alanine. Transgenes encoding mutant Pto(G2A) and wild-type were placed behind cauliflower mosaic virus 35S promoter transformed into plants are...