A5058703954- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Nanoparticles: synthesis and applications
- Nanowire Synthesis and Applications
- Animal Nutrition and Physiology
- Pharmacological Effects and Assays
- Spectroscopy and Chemometric Analyses
- Silicon Nanostructures and Photoluminescence
- Polyamine Metabolism and Applications
- Meat and Animal Product Quality
- Tea Polyphenols and Effects
- Amino Acid Enzymes and Metabolism
- Advanced Cellulose Research Studies
- Electrochemical sensors and biosensors
Consejo Nacional de Humanidades, Ciencias y Tecnologías
2023
Instituto Politécnico Nacional
2016-2023
Autonomous University of Tlaxcala
2016
An easily prepared fluorescent bioconjugate colloidal solution based on gold nanoparticles is reported for the detection of Salmonella. This was by conjugation staphylococcal protein A surface nanoparticles, followed biofunctionalization over this layer with fluorescein isothiocyanate-labeled antibodies. The plasmon resonance band observed in ultraviolet–visible spectrum showed a shift to low energy after covering and broadening antibody. Surface-enhanced infrared spectroscopy absorption...
A practical method to determine Staphylococcus aureus using bioconjugated gold nanoparticles is reported. The protocol uses stabilized by tetramethylrhodamine isothiocyanate-labeled streptavidin followed functionalization with biotinylated anti-S. antibodies. streptavidin-stabilized were obtained titration and analyzed ultraviolet–visible spectroscopy transmission electron microscopy. fluorescent bioconjugate selectively linked the surface of S. in samples contaminated microorganism, as...
Development of two biosensors to detect biogenic amines was carried out using immobilized diamine oxidase from Pisum sativum on 3-APTMS-functionalized SiO2 substrates with either glutaraldehyde or terephthalaldehyde as crosslinkers. The assembly process validated by FT-IR, SEM-EDX, and AFM. Biosensor responses were c.a. 3-fold lower compared the free enzyme kinetics assessed UV/VIS spectrophotometry. calculated initial velocities (ΔAbs·min-1) for in given order (3.2 ± 0.68) × 10–2, (0.48...
Green synthesis of gold nanoparticles using an aqueous coffee (<i>Coffea arabica</i>) pulp extract as a reducing agent was achieved and analyzed by response surface methodology, given the diverse applicability these containing wide range phenolic compounds from extract. Three factors (precursor concentration, reaction time) were considered their combined effects on maximum intensity (<i>MA</i>), particle size (<i>d</i>), density (<i>N</i>)...
La detección de agentes químicos y biológicos juega un papel importante en áreas como la física, química, biología, medicina nanotecnología. Esta última incorpora el uso nanomateriales las nanopartículas oro, cuales presentan propiedades fisicoquímicas importantes para desarrollo nuevas metodologías reconocimiento transducción sensores biológicos. Algunas más relevantes oro son eléctricas, ópticas biocompatibilidad, además que poseen área superficial amplia, permite inmovilización proteínas...
Bacterial cellulose membranes were synthesized by liquid fermentation of the Kombucha strain into black tea (Camellia sinensis) at different concentrations sucrose. Structural properties bacterial Kombucha, such as lateral order index (LOI), total crystallinity (TCI), hydrogen bond intensity (HBI), fraction (fa), well their dependence on sucrose content evaluated Fourier transform infrared spectroscopy, using absorption bands vibrational spectrum. It was thus observed that tends to...