A5034844856- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Cancer Mechanisms and Therapy
- Endoplasmic Reticulum Stress and Disease
- Cardiac pacing and defibrillation studies
- Microbial Natural Products and Biosynthesis
- Cancer-related gene regulation
- Transplantation: Methods and Outcomes
- Protein Tyrosine Phosphatases
- Heat shock proteins research
- Chronic Lymphocytic Leukemia Research
- Advanced Mathematical Modeling in Engineering
- Protein Degradation and Inhibitors
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Advanced Breast Cancer Therapies
- Fungal and yeast genetics research
- Heart Failure Treatment and Management
Harvard University
2015-2021
University of Arizona
2011-2014
Qualcomm (United States)
2014
To achieve maximal growth, cells must manage a massive economy of ribosomal proteins (r-proteins) and RNAs (rRNAs) to produce thousands ribosomes every minute. Although are essential in all cells, natural disruptions ribosome biogenesis lead heterogeneous phenotypes. Here, we model these perturbations Saccharomyces cerevisiae show that challenges result acute loss proteostasis. Imbalances the synthesis r-proteins rRNAs rapid aggregation newly synthesized orphan compromise cellular processes,...
Heat shock factor 1 (Hsf1) activation is responsible for increasing the abundance of protein-folding chaperones and degradation machinery in response to proteotoxic conditions that give rise misfolded or aggregated proteins. Here we systematically explored link between concurrent protein synthesis stress budding yeast, Saccharomyces cerevisiae. Consistent with prior work, inhibiting before inducing prevents Hsf1 activation, which demonstrated across a broad array stresses validate using...
The activity of protein kinases are naturally gated by a variety physiochemical inputs, such as phosphorylation, metal ions, and small molecules. In order to design that can be user-defined we describe sequence dissimilarity based approach for identifying sites in accommodate 25-residue loop insertion while retaining catalytic activity. We further demonstrate the successful mutants provide guidance dissection into two fragments cannot spontaneously assemble thus inactive but converted...
Protein kinases phosphorylate client proteins, while protein phosphatases catalyze their dephosphorylation and thereby in concert exert reversible control over numerous signal transduction pathways. We have recently reported the design validation of split-protein that can be conditionally activated by an added small molecule chemical inducer dimerization (CID), rapamycin. Herein, we provide rational three split-tyrosine (PTPs) attached to FKBP FRB, where catalytic activity modulated with...
Remote monitoring for heart failure (HF) has had mixed and heterogeneous effects across studies, necessitating further evaluation of remote systems within specific healthcare their patient populations. "Care Beyond Walls Wires," a wireless program to facilitate care team co-management HF patients, served by rural regional medical center, provided the opportunity evaluate this on utilization.Fifty patients admitted Flagstaff Medical Center (Flagstaff, AZ) participated in project. Many these...
Abstract To achieve maximal growth, cells must manage a massive economy of ribosomal proteins (r-proteins) and RNAs (rRNAs), which are required to produce thousands new ribosomes every minute. Although essential in all cells, disruptions ribosome biogenesis lead heterogeneous phenotypes. Here, we modeled these perturbations Saccharomyces cerevisiae show that challenges result immediately acute loss proteostasis (protein folding homeostasis). Imbalances the synthesis r-proteins rRNAs rapid...
Regulation of mitochondrial gene expression is essential to prevent accumulation harmful intermediates at respiratory chain reaction centers that cause aging phenotypes and disease. Translation in mitochondria carried out by dedicated ribosomes (mitoribosomes) are distinct from cytoplasmic ribosomes. The translational response environmental developmental changes currently unexplored because tools have not been developed for quantitative, time‐resolved studies vivo. Genomic approaches...