A5068614239- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Plant Virus Research Studies
- HIV Research and Treatment
- Evolution and Genetic Dynamics
- Animal Disease Management and Epidemiology
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Vector-Borne Animal Diseases
- Virus-based gene therapy research
- Parvovirus B19 Infection Studies
- HIV/AIDS drug development and treatment
- Herpesvirus Infections and Treatments
- COVID-19 Impact on Reproduction
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- RNA and protein synthesis mechanisms
The Francis Crick Institute
2013-2016
University of London
2013
Centro de Biología Molecular Severo Ochoa
2008-2012
Consejo Superior de Investigaciones Científicas
2012
Universidad Autónoma de Madrid
2008-2012
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2010-2012
Institut Pasteur
2011-2012
Based on structural data of the RNA-dependent RNA polymerase, rational targeting key residues, and screens for Coxsackievirus B3 fidelity variants, we isolated nine polymerase variants with mutator phenotypes, which allowed us to probe effects lowering virus replication, mutability, in vivo fitness. These strains generate higher mutation frequencies than WT are more sensitive mutagenic treatments, their purified polymerases present lower-fidelity profiles an vitro incorporation assay....
Human immunodeficiency virus (HIV) and human cytomegalovirus (HCMV) coinfections have been shown to increase infant morbidity, mortality, AIDS progression. In HIV-endemic regions, maternal HIV-exposed but HIV-uninfected infants, which is the majority of children affected by HIV, also show poor growth increased morbidity. Although nutrition has examined, effects HCMV infection not evaluated. We studied on growth, development, health maternally unexposed infants in Zambia.Infants were examined...
The characterization of virulence determinants pathogenic agents is utmost relevance for the design disease control strategies. So far, two classes have been characterized viral populations: those imprinted in nucleotide sequence some specific genomic regions and that depend on complexity population as such. Here we provide evidence a determinant depends neither nor detectable differences complexity. Foot-and-mouth virus lethal C57BL/6 mice showing highest load pancreas. Virus isolated from...
RNA viruses use dependent polymerases to replicate their genomes. The intrinsically high error rate of these enzymes is a large contributor the generation extreme population diversity that facilitates virus adaptation and evolution. Increasing evidence shows intrinsic rates, resulting mutation frequencies, can be modulated by subtle amino acid changes viral polymerase. Although biochemical assays exist for some permit quantitative measure incorporation fidelity, here we describe simple...
The Spumaretrovirinae, or foamyviruses (FVs) are complex retroviruses that infect many species of monkey and ape. Although FV infection is apparently benign, trans-species zoonosis commonplace has resulted in the isolation Prototypic Foamy Virus (PFV) from human sources potential for germ-line transmission. Despite little sequence homology, orthoretroviral Gag proteins perform equivalent functions, including genome packaging, virion assembly, trafficking membrane targeting. In addition, PFV...
The Spumaretrovirinae, or foamy viruses (FVs) are complex retroviruses that infect many species of monkey and ape. Despite little sequence homology, FV orthoretroviral Gag proteins perform equivalent functions, including genome packaging, virion assembly, trafficking membrane targeting. However, there is a paucity structural information for FVs it unclear how disparate molecules share the same function. To probe functional overlap we have determined structure central region from Prototype...
Abstract Foot-and-mouth disease virus (FMVD), one of the most contagious viruses cloven-hoofed animals, may cause a prolonged, asymptomatic but persistent infection in ruminants, named "carrier state". However, it remains an open question whether this carrier state occurs pigs. Here we present quantitative analyses duration FMDV RNA and infectivity lymphoid epithelial tissues experimentally infected pigs with C-S8c1. The data indicated that although remained blood until day 14 post-infection...
Low fidelity replication and the absence of error-repair activities in RNA viruses result complex adaptable ensembles related genomes viral population, termed quasispecies, with important implications for natural infections. Theoretical predictions suggested that elevated error rates might be near to a maximum compatible viability. This fact encouraged use mutagenic nucleosides as new antiviral strategy induce extinction through increased rates. Despite extensive evidence lethal mutagenesis...
Background New vaccine designs are needed to control diseases associated with antigenically variable RNA viruses. Foot-and-mouth disease (FMD) is a highly contagious of livestock that inflicts severe economic losses. Although the current whole-virus chemically inactivated has proven effective, it led new outbreaks FMD because incomplete inactivation virus or escape infectious from production premises. We have previously shown serial passages (FMDV) C-S8c1 at high multiplicity infection in...
Background RNA virus populations are heterogeneous ensembles of closely related genomes termed quasispecies. This highly complex distribution variants confers important properties to viruses and influences their pathogenic behavior. It has been hypothesized that increased mutagenesis viral populations, by treatment with mutagenic agents, can induce alterations in the potential a population. In this work we investigate whether mutagenized foot-and-mouth disease (FMDV) display changes...
RNA viruses use dependent polymerases to replicate their genomes. The intrinsically high error rate of these enzymes is a large contributor the generation extreme population diversity that facilitates virus adaptation and evolution. Increasing evidence shows intrinsic rates, resulting mutation frequencies, can be modulated by subtle amino acid changes viral polymerase. Although biochemical assays exist for some permit quantitative measure incorporation fidelity, here we describe simple...
Background Human prototypic foamy virus (HPFV) belongs to the spumaretrovrinae subfamily and is an attractive vector candidate for gene therapy [1] as it apathogenic. The Gag protein not cleaved into matrix (MA), capsid (CA) nucleocapsid (NC) occurs in orthoretroviruses; rather, able perform roles of these proteins a single polypeptide [2]. Foamy are targets restriction factors such Trim5a [3] also interact with aminoterminal leader peptide envelope (Env). This Gag-Env interaction essential...