Abstract Current extraction methods often extract DNA and RNA separately, few are capable of co-extracting from sputum. We established a nucleic acid co-extraction method sputum based on magnetic beads optimized the by evaluating influencing factors, such as guanidinium thiocyanate (GTC) dithiothreitol (DTT) concentrations, bead amount, incubation temperature, lysis buffer pH carrier type. The feasibility simultaneous was evaluated amplifying viruses single clinical specimen with multiplex...

This study investigated the distribution of antibiotic resistant Escherichia coli (E. coli) and examined possible relationship between water quality parameters resistance from two different drinking sources (the Qiantang River Dongtiao Stream) in Hangzhou city China. E. isolates were tested for their susceptibility to eighteen antibiotics. Most tetracycline (TE), followed by ampicillin (AM), piperacillin (PIP), trimethoprim/sulfamethoxazole (SXT) chloramphenicol (C). The rate was similar;...

Acinetobacter baumannii (A. or AB) is one of the most opportunistic, nosocomial pathogens threatening public healthcare across countries. A. has become a primary growing concern due to its exceptional ability acquire antimicrobial resistance (AMR) multiple agents which increasingly reported and more prevalent every year. Therefore, there an urgent need evaluate AMR knowledge for effective clinical treatment infections. This study aimed investigate distribution phenotypes genotypes, genomic...

Carbapenem-resistant Klebsiella pneumoniae (CRKp) infection poses a significant global public health challenge, with the misuse of antibiotics further contributing to development resistance and triggering harmful inflammatory responses. Outer membrane vesicles (OMVs) released by CRKp under sub-lethal concentration MEM pressure (KOMV-MEM) exhibit enhanced virulence greater efficiency in transferring genes. We investigated inhibitory effects chlorogenic acid (CA) on KOMV-MEM characteristics...

Hypermethylation of the promoter region RAS association domain family 1A gene (RASSF1A) occurs widely in hepatocellular carcinoma (HCC) tissues. While diagnostic performance use RASSF1A methylation as a serum or plasma marker patients with HCC has varied largely literature,we confirmed clinical application value for HBV related this study. A total 584 participants were recruited into study, including 190 HCC, 114 liver cirrhosis (LC), 120 chronic hepatitis B (CHB) and 160 healthy...

Central nervous system (CNS) involvement in diffuse large B-cell lymphoma (DLBCL) is relatively rare, occurring at a rate of approximately 5%. Primary CNS (CNS-DLBCL), subtype DLBCL, rare clinically but highly malignant and invasive. Its atypical clinical symptoms imaging features contribute to high misdiagnosis poor prognosis. Thus, early accurate diagnosis imperative for improving the patient's Cerebrospinal fluid (CSF) cytology, rapid convenient diagnostic method, plays crucial role...

Penicillin-resistant Streptococcus pneumoniae (PRSP), for which novel treatment medicines are required, has expanded extensively due to the overuse of antibiotics. This study aimed detect optimal ratio combination main components based on Yinhuapinggan granules (YHPG) generate concepts PRSP-induced lung injury. Three representative components: chlorogenic acid (C), amygdalin (A), and puerarin (P) were selected, these three was determined by an orthogonal experiment. Investigations conducted...

To investigate the in vitro and vivo antibacterial activities of tigecycline other 13 common antimicrobial agents, alone or combination, against multi-drug resistant Acinetobacter baumannii.An susceptibility test 101 A. baumannii was used to detect minimal inhibitory concentrations (MICs). A mouse lung infection model baumannii, established by ultrasonic atomization method, define activities.Multi-drug showed high sensitivity (98% inhibition), polymyxin B (78.2% minocycline (74.2%...

To discuss the clinical significance of interleukin (IL)-6 in differential diagnosis sepsis and its capability differentiating induced by Gram-negative bacteria from that Gram-positive bacteria.A total 379 children with were involved this study to form case group, their C-reactive protein (CRP), procalcitonin (PCT) IL-6 levels before antibiotics after recovery checked. Receiver operating characteristic curve was applied evaluate CRP, PCT bacteria.When these 3 indicators sepsis, area under...

Glycyrrhetinic acid (GA) is a bio-effective component of Licorice. The GA monomer and the ingredient an Oleanane-type pentacyclic triterpenes that has been used as remedy for years. Due to abuse antibiotics, people pay attention emergence Multidrug-resistant Acinetobacter baumannii (MDR-AB). As conditional pathogen, MDR-AB causes severe infection, endangering human lives. Our previous studies found played important role in Yinhua Pinggan, Chinese medicine. However, whether could protect lung...

Acinetobacter baumannii is a type of bacterial nosocomial infection with severe drug resistance. Hemolysin co-regulated protein (Hcp) marker activated VI secretion system (T6SS), key secretory that promotes Gram-negative bacteria colonization, adhesion, and invasion host cells. Hcp also regulated by iron ions (Fe). In this study, an ATCC17978 hcp deletion strain (ATCC17978Δ ), complement + A. –green fluorescent (GFP) were constructed used to investigate the role in adhesion cells (human...

Background Human papillomaviruses (HPV) are classified into high-risk HPV and low-risk HPV. The most common types in cervical cancer 16 18, the causing genital warts 6 11. In this study, applying novel AllGlo fluorescent probes, we established a quadruplex quantitative PCR method to simultaneously detect differentiate 6, 11, 18 single tube. Methods specificity, sensitivity, detection limit, reproducibility standard curve of were examined. Finally, clinical samples that had been tested...

The aim of the present study was to investigate prevalence BRAF V600E mutation in papillary thyroid carcinoma (PTC) patients from eastern coastal China and determine whether it is correlated with clinicopathological features PTCs or without current Hashimoto thyroiditis (HT). status analyzed 206 nodules 154 undergoing thyroidectomy using polymerase chain reaction bi‑directional sequencing. Multivariate analysis performed association features. Thyroid were classified as PTC, nodular goiter...

Objective . Since early diagnosis is very important for treating gastric cancer (GC), we aimed to detect serum small proline-rich protein2A (SPRR2A) verify its diagnostic value GC patients. Methods Serum samples were collected from 200 patients with GC, 100 gastritis, 40 rectal (RC), 50 colon (CC), and healthy controls. An enzyme-linked immunosorbent assay (ELISA) detection kit was applied measure SPRR2A concentration. The correlations between carcinoembryonic antigen (CEA), clinical...

Multiplex quantitative polymerase chain reaction (qPCR) has found an increasing range of applications. The construction a reliable and dynamic mathematical model for multiplex qPCR that analyzes the effects interactions between variables is therefore especially important. This work aimed to analyze through response surface method (RSM) uni- qPCR, further optimize parameters by constructing two models via RSM back-propagation neural network-genetic algorithm (BPNN-GA) respectively....

<h3>BACKGROUND:</h3> The aim of this study was to assess insulin-like growth factor-1 (IGF-1) and factor-binding protein-3 (IGFBP-3) concentrations in children with obstructive sleep apnea-hypopnea syndrome (OSAHS) compare the results according disease severity duration. <h3>METHODS:</h3> Fifty-one OSAHS underwent polysomnography were classified as having mild, moderate, or severe disease. All bilateral tonsillectomy/adenoidectomy adenoidectomy. IGF-1 IGFBP-3 assessed before surgery 3 6...

Acute respiratory infections (ARIs) cause substantial morbidity and mortality worldwide. The causes of ARI are dynamic, co-infections Mycoplasma pneumoniae, Epstein–Barr virus human cytomegalovirus recently developed ARI. Here, we established a quadruplex quantitative PCR (qPCR) method to rapidly identify simultaneously detect single infection or co-infection these three pathogens an internal control in tube using AllGlo probes. analysis demonstrated wide linear range detection from 101 108...