A5008473311- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Advancements in Transdermal Drug Delivery
- Ocular Surface and Contact Lens
- Genomics and Chromatin Dynamics
- Skin Protection and Aging
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Dermatology and Skin Diseases
- Synthesis and Biological Evaluation
- Advanced Glycation End Products research
- Pregnancy and Medication Impact
- Blood properties and coagulation
- Genomics, phytochemicals, and oxidative stress
- Contact Dermatitis and Allergies
- Adolescent and Pediatric Healthcare
- Proteins in Food Systems
- Adenosine and Purinergic Signaling
University College London
2018-2025
The London College
2019-2025
Royal Veterinary College
2017
Abstract Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a key regulator of cell detoxification, which maintains homoeostasis in healthy cells and promotes chemoresistance cancer cells. Controlling the expression this transcription therefore great interest. There are many compounds that have been shown to induce Nrf2 expression, but ligands can inhibit scant. Herein we characterise an i-motif-forming sequence downstream promoter, hypothesised may regulate gene. The i-motif was found be...
Abstract Sequences that form DNA secondary structures, such as G‐quadruplexes (G4s) and intercalated‐Motifs (iMs), are abundant in the human genome play various physiological roles. However, they can also interfere with replication threaten stability. Multiple lines of evidence suggest G4s inhibit replication, but underlying mechanism remains unclear. Moreover, how iMs affect replisome is lacking. Here, we reconstitute physiologically derived structure‐forming sequences to find a single G4...
i-Motifs (iMs), are secondary structures formed in cytosine-rich DNA sequences and involved multiple functions the genome. Although putative iM forming widely distributed human genome, folding status strength of iMs vary dramatically. Much previous research on has focused assessing properties using biophysical experiments. However, there no dedicated computational tools for predicting structures. Here, we introduce a machine learning pipeline, iM-Seeker, to predict both structural stability...
The naphthalene diimide compound QN-302, designed to bind G-quadruplex DNA sequences within the promoter regions of cancer-related genes, has high anti-proliferative activity in pancreatic cancer cell lines and anti-tumor several experimental models for disease. We show here that QN-302 also causes downregulation expression S100P gene protein cells vivo. This is well established as being involved key proliferation motility pathways human cancers been identified a potential biomarker cancer....
The insulin-linked polymorphic region is a variable number of tandem repeats DNA in the promoter insulin gene that regulates transcription insulin. This known to form alternative structures, i-motifs and G-quadruplexes. Individuals have different sequence variants although previous work investigated effects some on G-quadruplex formation, there not clear picture relationship between diversity, structures formed, functional expression. Here we show linked vitro. Additionally, reporter genes...
Abstract DNA, beyond its canonical B-form double helix, adopts various alternative conformations, among which the i-motif, emerging in cytosine-rich sequences under acidic conditions, holds significant biological implications transcription modulation and telomere biology. Despite recognizing crucial role of i-motifs, predictive software for i-motif forming has been limited. Addressing this gap, we introduce ‘iM-Seeker’, an innovative computational platform designed prediction evaluation...
Bone cells constitutively release ATP into the extracellular environment where it acts locally via P2 receptors to regulate bone cell function. Whilst P2Y2 receptor stimulation regulates mineralisation, functional effects of this in osteoclasts remain unknown. This investigation used knockout (P2Y2R-/- ) mouse model investigate role bone. MicroCT analysis P2Y2R-/- mice demonstrated age-related increases trabecular volume (≤48%), number (≤30%) and thickness (≤17%). In vitro osteoblasts...
ABSTRACT The insulin linked polymorphic region (ILPR) is a variable number of tandem repeats (VNTR) DNA in the promoter gene that regulates transcription insulin. This known to form alternative structures, i-motifs and G-quadruplexes. Individuals have different sequence variants VNTR although previous work investigated effects some on G-quadruplex formation, there not clear picture relationship between diversity, structures formed, functional expression. Here we show ILPR secondary...
GC-rich sequences can fold into G-quadruplexes and i-motifs are known to control gene expression in many organisms. The potent G-quadruplex experimental anticancer drug QN-302 down-regulates a number of cancer-related genes, particular
The maturity of the corneocyte envelope (CE) provides information about barrier functionality stratum corneum (SC). Corneocytes are enclosed by CE, a protein-lipid matrix, contributing to mechanical resistance and hydrophobicity SC.
Terminally differentiated keratinocytes acquire corneocyte protein envelopes (CPE) complexed with lipid (CLE). These two structural components of the (CEs) undergo maturation by gaining in hydrophobicity, rigidity and surface area. Linoleoyl acylceramides are processed 12R-lipoxygenase (12R-LOX) other enzymes before transglutaminase (TG) attaches ω-hydroxyceramides to involucrin CPE. Concurrently, proteins cross-linked TG that has been activated cathepsin D (CathD).The primary aim this work...
There are thousands of compounds shown to interact with G-quadruplex DNA, yet very few which target i-motif (iM) DNA. Previous work showed that tobramycin can iM- indicating the potential for sugar-molecules these structures. Computational approaches indicated sugar-containing natural products baicalin and geniposidic acid had iM-DNA. We assessed DNA interacting properties using FRET-based melting a fluorescence-based displacement assay iM-DNA structures from human telomere insulin linked...
During the late stage of keratinocyte differentiation, corneocytes gain a strong protein-lipid structure: corneocyte envelopes (CE), composed inner protein envelope (CPE) and outer lipid (CLE). The hydrophobicity CEs depends on covalent attachment linoleoyl-acyl-ceramides by transglutaminases (TG). These ceramides are processed range other enzymes, including 12R-lipoxygenase (12R-LOX), before free ω-hydroxyceramides to CPE surface form CLE. mechanical strength CE is obtained with formation...
Abstract Sequences that can form DNA secondary structures, such as G-quadruplexes (G4s) and intercalated-Motifs (iMs), are abundant in the human genome play a range of physiological roles. However, they also pose challenge to replication machinery turn threaten stability. Multiple lines evidence suggest G4s interfere with replication, but underlying mechanism remains unclear. Moreover, there is lack how iMs affect replisome. Here, we reconstitute physiologically derived structure-forming...
ABSTRACT i-Motifs (iMs), are secondary structures formed in cytosine-rich DNA sequences and involved multiple functions the genome. Although putative iM forming widely distributed human genome, folding status strength of iMs vary dramatically. Much previous research on has focused assessing properties using biophysical experiments. However, there no dedicated computational tools for predicting structures. Here, we introduce a machine learning pipeline, iM-Seeker, to predict both structural...