A5090261237- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Conducting polymers and applications
- Infant Nutrition and Health
- Electrochemical Analysis and Applications
- RNA Interference and Gene Delivery
- bioluminescence and chemiluminescence research
- Nitric Oxide and Endothelin Effects
- Dendrimers and Hyperbranched Polymers
- Cancer therapeutics and mechanisms
- Molecular Junctions and Nanostructures
- Acoustic Wave Resonator Technologies
Institute of Biocybernetics and Biomedical Engineering
2017-2024
Polish Academy of Sciences
2018-2024
There is growing interest for bioanalytical tools that might be designed a specific user, primarily research purposes. In this perspective, new, highly stable potentiometric sensor based on glassy carbon/polyazulene/NH4+-selective membrane was developed and utilized urease activity determination. Urease-urea interaction studies were carried out the Michaelis-Menten constant established enzymatic reaction. Biofunctionalization of ammonium ion-selective with lead to urea biosensor remarkably...
This paper presents a novel biosensor for detecting the active metabolite of irinotecan, SN38, with high selectivity and sensitivity. The utilizes dsDNA decamer (5'-GCGTTGTCGC-3') that selectively interacts SN38. biosensor, consisting screen-printed graphite electrode as recognition layer, demonstrates stability, sensitivity, towards SN38 through electrochemical studies. It offers wide linear range 1-200 nM, comparable to high-performance liquid chromatography (HPLC) method, sensitivity 57...
Abstract Pirarubicin (THP) is an anthracycline drug, which mainly used in the anticancer treatment. Utilization of THP may be associated with many dangerous side effects, therefore monitoring drug level patients’ organism important aspect chemotherapy. In our research we focused on designing electrochemical DNA‐based biosensor for selective detection pirarubicin (THP). Our utilizes ability to interact DNA double helix and fact that electrochemically active compound. Graphite electrodes...
Fluorescein is a fluorescent dye, that commonly used as label in biochemistry, environment protection and ophthalmology. Its features are well known, while the knowledge about its electrochemical behavior still negligible. In our research, we decided to investigate activity of fluorescein determine concentration with use differential pulse voltammetry spectrofluorimetry order compare utility each method for detection, towards simultaneous optical detection bioanalytes. Due strong effect pH...
In this work, new graphene-based screen-printed electrodes are proposed as a new, cheaper alternative to more common for determination of dopamine. Electrodes were designed, manufactured and tested look correlations between different graphene weight percentages used in oxidation peaks sensitivity dopamine determination. All using cyclic voltammetry. For reference, graphite tested. With 0.0161 μA/μM detection limit 5.549 μM those showed potential promise use electrode material. manufacturing,...
This paper presents a novel biosensor for detecting the active metabolite of irinotecan, SN38, with high selectivity and sensitivity. The utilizes dsDNA decamer (5’-GCGTTGTCGC-3’) that selectively interacts SN38. biosensor, consisting screen-printed graphite electrode as recognition layer, demonstrates stability, sensitivity, towards SN38 through electrochemical studies. It offers wide linear range 1-200 nM, comparable to high-performance liquid chromatography (HPLC) method, sensitivity 57...
The determination of the enzymatic activity requires constant and reproducible measuring conditions, therefore highly stable potentiometric biosensor operating on basis coupled enzyme reactions is proposed for arginase determination. Glassy carbon electrode was covered with polyazulene ion-to-electron transducing layer, which ensured improved stability prepared sensor. sensor’s selectivity obtained by applying NH 4 + -selective membrane further biofunctionalized urease via covalent...