Analytical applications often require rapid measurement of compounds from complex sample mixtures. High-speed mass spectrometry approaches frequently utilize techniques based on direct ionization the by laser irradiation, mostly means matrix-assisted desorption/ionization (MALDI). Compounds low molecular weight are difficult to analyze MALDI, however, because severe interferences in m/z range organic matrix used for desorption/ionization. In recent years, surface-assisted (SALDI) have shown...

Complex microvascular venation patterns of natural leaves are replicated into PDMS replicas, which allows for a leakage-tight seal with flat substrate despite the surface topography.

A simple approach for synthesis of palladium and silver nanostructures with readily adjustable morphologies was developed using galvanic electrochemical deposition, application to surface-assisted laser desorption/ionization (SALDI) small biological molecules. range fatty acids, triglycerides, carbohydrates, antibiotics were investigated assess the performance new materials. Intense analyte cations generated from surfaces upon UV irradiation such as potassium adducts a film thickness <100 nm...

Monitoring and control of fermentation processes remain a crucial challenge for both laboratory industrial-scale experiments. Reliable identification quantification the key process parameters in on-line mode allow operation at optimal reactor efficiency, maximizing productivity while minimizing waste. However, state-of-the-art monitoring is still limited to number standard measurements such as pH, temperature dissolved oxygen, well off-gas analysis an advanced possibility. Despite...

Abstract The development is proposed of a specific non-enzymatic amperometric sensor based on electrodeposited copper nanoparticles (Cu-NPs) for the determination uric acid (UA) in fermentation samples. Through optimization Cu-NPs-containing sensing layer, it was demonstrated that copper(II)-induced oxidation (catalytic effect) presence molecular oxygen more effective determining UA than adsorption Cu and Cu-oxide surfaces. More importantly, simply changing layer’s surface chemistry by...

Abstract Glycerol is a widely used signaling bioanalyte in biotechnology. can serve as substrate or product of many metabolic processes cells. Therefore, quantification glycerol fermentation samples with inexpensive, reliable, and rapid sensing systems great importance. In this work, an amperometric assay based on one-step designed electroplated functional Pd layers controlled design was proposed for selective measurement yeast medium. A novel utilizing Pd-sensing allows the medium presence...

In this study, the influence of surface morphology, reagent ions and restructuring effects on atmospheric pressure laser desorption/ionization (LDI) for small molecules after irradiation palladium self-assembled nanoparticular (Pd-NP) structures has been systematically studied. The dominant role morphology during LDI process, which was previously shown silicon-based substrates, not investigated metal-based substrates before. our experiments, we demonstrated that both presence reorganization...

Organic-inorganic hybrids (OIH) are considered to be a powerful platform for applications in many research and industrial fields. This review highlights the application of OIH chemical analysis, biosensors, environmental monitoring. A methodology toward metrological traceability measurement standardization demonstration role mathematical modeling biosensor design also presented. The importance development novel types biosensing is highlighted. Finally, current trends nanometrology nanobiosensors

NP-based enzymatic biosensors were prepared by the simultaneous encapsulation of glucose and alcohol oxidases, Nafion noble metal NPs<italic>via</italic>co-deposition from a phosphate multiple electrolyte on top sensor surface.

Understanding the biorecognition and transduction mechanisms is a key aspect in development of robust sensing technologies. Therefore, design tools analytical approaches that could allow gaining deeper insight into bio- electrochemical processes would significantly accelerate progress field biosensors. Herein, we present novel effective strategy for biosensor screening based on tandem monitoring individual system parameters droplet. The developed approach couples simultaneous...

Herein, a study dealing with progress on palladium (Pd) electrocatalysts for an efficient glycerol electrooxidation in model aqueous and real fermentation solutions special focus some physicochemical parameters (e.g., the impact of adsorption stage multiple species, presence oxygen, influence anodic limits Pd-size) was conducted. During course investigations by tandem optical oxygen minisensor cyclic voltammetry significant efficiency Pd at alkali pH yeast media revealed. The obtained...

Fundamental parameters influencing the ion-producing efficiency of palladium nanostructures (nanoparticles [Pd-NP], nanoflowers, nanofilms) during laser irradiation were studied in this paper. The immobilized on surface different solid inorganic carrier materials (porous and mono-crystalline silicon, anodic porous aluminum oxide, glass polished steel) by using classical galvanic deposition, electroless local deposition sputtering. It was goal study to investigate influence both...

In this study, we propose a simple and rapid technique for characterization of free fatty acids triacylglycerides (TAG) based on palladium nanoparticular (Pd-NP) surface-assisted laser desorption/ionization (SALDI) mass spectrometry (MS).

Quantum dots (QDs) have great potential as fluorescent labels but cytotoxicity relating to extra- and intracellular degradation in biological systems has be addressed prior biomedical applications. In this study, human intestinal cells (Caco-2) grown on transwell membranes were used study penetration depth, localization, translocation of CdSe/ZnS QDs with amino carboxyl surface modifications. The focus was compare the depth differentiated vs undifferentiated using confocal microscopy image...

In this study, fundamental aspects that have impact on the electroanalytical detection of hydrazine in phosphate, acetate and yeast fermentation medium an analytically significant concentration range by several types palladium (Pd)-modified electrodes, namely, Pd-ink, Pd-sputtered films nanoparticles (Pd-NPs) were systematically studied. The efficiency electrooxidation is not affected composition multicomponent (i), presence oxygen (ii), morphology or electroactive area (iii), but more...

Abstract Herein an assay toward a rapid and reliable profiling of extracellular matrix Escherichia coli (E. coli) utilizing tandem GC‐MS as tool for definition the exact chemical nature low molecular weight compounds cyclic voltammetry their high throughput detection is presented. Briefly, during set investigations formation glycerol in (ECM) E. at physiological relevant conditions cells was revealed. Based on obtained knowledge, electrochemical protocol allowing both qualitative...

The sorption of selected hydrophilic pH-sensitive dyes (bromophenol blue, bromothymol bromocresol purple, alizarin red, methyl orange, congo rhodamine 6G) on films anodized aluminium oxide (AAO) was investigated in this study. Depth and pore structure the AAO channels were adjusted by changing electrolysis time current density during treatment foil oxalic acid, sulfosalycilic acid sulfuric at concentration levels between 0.2 0.6 M. immobilized surface direct saturation dye solutions. It...