Vascular endothelial cells (VECs) injury is the first step in pathogenesis of atherosclerosis (AS). Mitochondrial dysfunction plays a significant role VECs injury, but underlying mechanisms are still unclear. Here, human umbilical vein were exposed to 100 μg/mL oxidized low-density lipoprotein for 24 h establish AS model vitro. We reported that mitochondrial dynamics disorder prominent feature models and associated with dysfunction. Moreover, knockdown dynamin-related protein 1 (DRP1)...

BACKGROUND The role of the positive end-expiratory pressure (PEEP) and lung recruitment manoeuvre (LRM) combination (termed open-lung strategy, OLS) during intra-operative mechanical ventilation is not clear. OBJECTIVE To determine whether an strategy constituting medium PEEP (6–8 cmH 2 O) repeated LRMs protects against postoperative complications in at-risk patients undergoing laparoscopic colorectal cancer resection under low-tidal-volume ventilation. DESIGN A prospective,...

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies worldwide with very limited treatment options. Cold-inducible RNA binding protein (CIRBP) plays promoting roles in several types cancers, but its function remains unclear PDAC. Here, we found that expression CIRBP was upregulated PDAC tumor tissues and significantly associated poor prognosis. Knockdown PANC-1 SW1990 cells inhibited proliferation, migration invasion vitro suppressed growth vivo . Moreover,...

Abstract Aims Regular transient limb ischemia (RTLI) can prevent atherosclerosis (AS) progression in hypercholesterolemic rabbits. This study aimed to investigate the minimum effective intensity and possible mechanisms of RTLI for preventing atherosclerosis. Methods Eighty rabbits were divided into eight groups: normal (N), high cholesterol (H), three [three cycles every other day (R3qod), daily (R3qd), six (R6qd), each cycle included 5 min followed by reperfusion], correlated sham [sham 30...

The postoperative length of hospital stay (PLOS) is an important indicator surgical quality. We identified perioperative factors that affect prolonged PLOS (PPLOS) after laparoscopic colorectal cancer resection, which the preferred approach for cancer, third most common cancer.This study was a secondary analysis randomized trial (clinicaltrials.gov ID: NCT03160144) included 280 patients who underwent resection. primary outcome PPLOS, defined as longer than median PLOS. Baseline, anesthetic,...

Endothelial dysfunction, the initial pathogenic factor in atherosclerosis, can be alleviated via transient limb ischemia. We observed effects of regular ischemia (RTLI) on atherosclerosis hypercholesterolemic rabbits.Twenty-eight rabbits were randomized to control, cholesterol, sham, groups (n = 7 each) between October 2010 and March 2011. They fed a normal diet control group other for 12 weeks. Six cycles RTLI performed once per day group. Serum samples prepared measure total cholesterol...

Administration of a single propofol bolus dose for anesthesia induction causes hypotension. We included 160 patients (74 males and 86 females; mean age, 42.4 ± 10.7 [range: 18–60] years) with the American Society Anesthesiologists status I–II undergoing elective surgery under general anesthesia. Using simple randomization, were divided into conventional group ( n = 80; received 2 mg/kg at rate 250 mg/min) titrated 1 mg/kg/min until Observer's Assessment Alertness/Sedation scale score reached...

e15194 Background: Sensitivity of chemotherapy is a great challenge to improve the treatment efficacy in patients with colorectal cancer (CRC). Increasing study has revealed role microbiota modulation sensitivity. However, mechanism how regulates 5-fluorouracil (5-FU) far from clear. Methods: We used germ-free mice and antibiotic-treated demonstrate impact gut on 5-FU Metabolomics analysis serum CRC or experiments were applied identify key metabolite, indole 3-propionic acid (IPA). Specific...

Background: The progression and metastasis of colorectal cancer (CRC) remain major clinical challenges due to a lack effective therapeutic targets. Our preliminary study identified the upregulation propionyl-CoA carboxylase alpha chain (PCCA) gene in CRC, prompting further investigation into its functional roles. Methods: Bioinformatics analysis, tumor tissues, CRC cell lines were used determine PCCA expression. Wound healing, Transwell, counting kit-8 (CCK-8) assays conducted evaluate...

Regular transient limb ischemia (RTLI) can prevent atherosclerosis in hypercholesterolemic rabbits. As endothelial dysfunction is the initial factor leading to atherosclerosis, we investigated effect of RTLI on function We randomly allocated 15 New Zealand white rabbits three groups, five animals per group: group (Group H), sham S), and L). All received fodder daily. No intervention was performed Group H. Rabbits S were kept hutches, with a deflated cuff applied their left hind for 60 min...

Objective: The objective is to investigate the incidence and consequences of arterial line occlusion during invasive blood pressure monitoring in form questionnaire survey. Methods: questionnaires were randomly distributed anesthesiologists who participated 22nd annual meeting Chinese society anesthesiology. main contents this survey included managing status, occlusion, effects on anesthesiologists’ therapy caused by occlusion. Results: Totally 294 collected, which 261 valid. In all...

Background & Objectives: Invasive blood pressure (IBP), the gold standard of arterial measurement[1], provides important guarantee for patients who are critically ill or undergoing high-risk and major surgery during perioperative period. Lots factors could lead to line occlusion IBP monitoring, which is a threat patients’ safety. Our aim was investigate managing status as well incidence, influence consequences invasive monitoring. Materials Methods: The questionnaire randomly distributed...

Objective To analyze the factors of maternal hypotension undergoing elective cesarean section with combined spinal epidural anesthesia (CSEA), and to investigate prevention method. Methods A retrospective study was conducted in 240 pregnant women ASA Ⅰ-Ⅱ CSEA, who were admitted The Third Affiliated Hospital Guangzhou Medical University from June 2010 May 2010. dependent variable person-time hypotension, 18 independent variables capacity control, usage phenylephrine, variety...

Objective To analyze the risk factors of combined spinal and epidural anesthesia (CSEA) for pregnant women with full stomach in emergency cesarean section. Methods A retrospective study was conducted 220 undergoing ASAⅠ-Ⅱcesarean section CSEA, including 136 cases under emergent (full group) 84 elective (control group), who were admitted to Third Affiliated Hospital Guangzhou Medial University from January 2010 2012. Partial correlation analysis used pregnancy stomach, such as nausea,...

To explore the effects of propofol sedation on psychological stress in patients undergoing surgery under epidural anesthesia.Sixty scheduled to undergo elective ileostomy closure anesthesia were randomized into group and control (n=30). The received a loading dose 0.6 mg·kg- 1· h- 1 followed by maintenance with continuous infusion 3 given after Observer's Assessment Alertness/Sedation (OAA/S) score reached 2-3. An equivalent volume normal saline was administered group. patients' preoperative...